表皮生长因子对肝星状细胞基质分解素-1及其抑制因子基因表达调节的影响

目的:了解表皮生长因子(EGF)对肝星状细胞基质分解素-1( MMP3)及金属蛋白酶组织抑制因子-1(TIMP1)基因表达的影响.方法:在培养的肝星状细胞系中加入EGF,于不同的时间点收集细胞,提取总RNA;用逆转录定量PCR方法测定基质分解素-1及TIMP1的基因表达水平.结果:EGF组肝星状细胞MMP3基因表达水平在8 h、24 h、48 h、72 h 4个时点均明显高于对照组;24 h达高峰,为对照组的3倍.EGF组肝星状细胞TIMP1的基因表达水平在上述几个时点亦明显高于对照组;24 h达高峰,为对照组的2倍.结论:EGF在体外可增强肝星状细胞基质分解素-1及TIMP1基因的表达.

中华眼镜蛇毒口服血清制剂对HepG2细胞凋亡的影响

目的:观察中华眼镜蛇毒口服血清制剂对人肝癌细胞凋亡的影响. 方法:HE染色、TUNEL、琼脂糖凝胶电泳、流式细胞术、免疫组化.结果:中华眼镜蛇毒口服血清制剂以剂量依赖方式显著诱导HepG2细胞的凋亡. 可见:细胞核浓缩,细胞体积缩小;TUNEL 阳性细胞明显增多;DNA呈现典型"梯形"变化;c-jun, c-myc基因表达明显升高.结论:中华眼镜蛇毒口服血清制剂诱导人肝癌细胞的凋亡,可能与c-jun, c-myc基因的表达上调有关.

IL-6、IL-8、TNF-α与支气管哮喘血液流变性异常关系的研究

目的:探讨支气管哮喘病人血液流变性异常与血清IL-6、IL-8、TNF-α间的关系.方法: 检测急性期和缓解期中、重度哮喘血液流变学指标以及血清IL-6、IL-8、TNF-α水平变化,并进行直线相关分析.结果: 哮喘病人急性期全血表现粘度(WBV)、血浆粘度(ηp)、红细胞聚集指数(PAI)均明显高于缓解期和正常对照组,氧释放系数(OD)则明显低于缓解期;缓解期ηp、RAI、OD值恢复至正常水平,但WBV仍高于正常水平.哮喘急性期血清IL-6、TNF-α均显著高于缓解期和正常对照组水平,缓解期二者下降至正常水平;急性期和缓解期血清IL-8均明显高于正常对照组.急性期和缓解期血清IL-8、TNF-α与WBV呈正相关.结论: 中、重度哮喘病人存在严重的血液流变学异常和细胞因子失调.IL-8、TNF-α可能参与了哮喘血液流变性异常的形成过程.

急、慢性肾功能衰竭血清铁蛋白的研究

目的: 评估急、慢性肾功能衰竭的血清铁蛋白浓度,建立一种新的鉴别诊断标准.方法: 随机选择35名急性肾功能衰竭(简称急性肾衰)患者,和35名慢性肾功能衰竭(简称慢性肾衰)患者分成两组,急性肾衰A组和慢性肾衰B组,并选30名正常人为C组和20名急性上呼吸道感染者为D组作为对照,测定血尿素氮(BUN)、血清肌酐(Scr)、红细胞压积(Hct)、血红蛋白(Hb)、血清铁蛋白(SF)和血清铁(SI)水平.结果:A组SF水平明显高于B、C组(P＜0.01),B组与C组SF无明显差异(P＞0.05).结论: 急性肾功能衰竭患者血清铁蛋白增高,慢性肾功能衰竭患者血清铁蛋白无明显变化.

实验性精索静脉曲张大鼠睾丸组织和血清一氧化氮和一氧化氮合酶的改变

目的:检测实验性精索静脉曲张大鼠睾丸组织和血清NO含量和NOS活性的改变,以进一步阐明精索静脉曲张的发生机制.方法:通过手术方法建立大鼠精索静脉曲张模型,检测NO和NOS的变化.结果:实验组左侧睾丸组织NO含量显著高于对照组左侧睾丸含量;实验组血清NOS活力明显低于对照组.结论:实验性精索静脉曲张大鼠睾丸组织和血清NO和NOS有改变.

Losartan对慢性低氧大鼠肺动脉压力及管壁胶原的影响

目的:探讨氯沙坦(losartan)对慢性低氧大鼠肺动脉压力及管壁胶原的影响.方法:将二级SD大鼠分为:对照组(A),低氧组(B),低氧+losartan组(C),低氧时间为4周.采用透射电镜、免疫组化、原位杂交等方法观察losartan对慢性低氧大鼠肺动脉平均压(mPAP)、右心室重量比(RV/LV+S)、肺细小动脉超微结构、肺动脉管壁Ⅰ、Ⅲ胶原和Ⅰ、Ⅲ前胶原基因的影响.结果:①B组mPAP 、RV/LV+S显著高于A组(P＜0.01),C组mPAP、RV/LV+S显著低于B组(P＜0.01).②电镜检查显示B组肺动脉胶原纤维较A组明显为多,C组较B组明显为少.③免疫组化、原位杂交显示B组肺细小动脉(直径约100-200 μm)Ⅰ型胶原及Ⅰ型前胶原mRNA平均吸光度值显著高于A组(均P＜0.01),C组肺细小动脉Ⅰ型胶原及Ⅰ型前胶原mRNA平均吸光度值明显低于B组(均P＜0.01),Ⅲ型胶原及Ⅲ型前胶原mRNA平均吸光度值各组间无明显差异(均P＞0.05).结论:losartan可预防慢性低氧肺动脉高压的形成和肺动脉管壁胶原的生成、沉积.

急性呼吸窘迫综合征患者血液中von Willebrand因子含量的变化

目的:观察21例急性呼吸窘迫综合征(acute respiratory distress syndrome, ARDS)患者发病早期血液中血管性假血友病因子(von Willebrand factor, vWF)含量的变化,考察其对ARDS早期诊断价值.方法: 21例ARDS患者第1 d,30例急性左心衰竭、慢性阻塞性肺病(chronic obstructive pulmonarydisease, COPD)肺部感染患者及正常对照同期抽血测vWF.结果: ARDS患者血vWF为325%±63%,急性左心衰竭患者血vWF为107%±42%,COPD肺部感染患者血vWF为103%±48%,正常对照者为92%±36%.ARDS组明显高于各对照组(P＜0.01).结论: ARDS患者在发病早期血vWF即明显升高,提示血vWF含量对ARDS的早期诊断有参考价值.

DNA芯片及其在医学上的应用

The development of DNA chip has been strongly driven by modern approaches of life science and microelectronics. The broad of DNA chip applications include the detection of pathogens,the detection of mutations in genes involved in human diseases or affected during cancer progression, sequence analysis, the pattern of gene expression monitoring and pharmacy studies .This review explains the technology, its scope, and impact on medicine, as well as its cost and possible limitations.

应激研究进展

The initial definition of stress and its development were briefly retrospected with elucidating the significance of stress in life sciences study. Stress is involved in a variety of physiological and pathophysiological processes. Description of the advances focusing on the relationships between stress and several body systems including nervous system, immune system and cardiovascular system etc. and on the stress molecules and signal transduction was carried out. The ultimate aim of the review is to emphasize the importance and the distinct position of stress during the development of modern bio-medicine, and to further attract more attention to the research field of stress from more scientists.

血小板反应素-1抑制肿瘤血管生成机制的研究进展

Inhibition of tumor neovascularization (angiogenesis) has become one of the novel therapeutic strategies for tumor, and natural inhibitor of angiogenesis has been highlighted in this field. Thrombospondin-1 (TSP-1), as an important extracellular matrix component which can influence endothelial cell adhesion, migration, and proliferation, is reported to be a potent inhibitor of angiogenesis in vivo. It will be valuable to use its active peptides or small fragments from TSP-1 in antiangiogenesis therapy.

碱性成纤维细胞生长因子与肝素结合性质的研究进展

Basic fibroblast growth factor (bFGF) is a multi-potential growth factor whose biological activities depend on its receptor's intrinsic tyrosine kinase activity and second messengers such as the mitogen activated protein kinases. Heparin sulfate proteoglycans (HSPGs) have been demonstrated to enhance or inhibit bFGF activity. The response elicited by HSPG is related to the relative concentrations and binding kinetics for bFGF of the various pools of HSPG. The type of cellular response might depend on the specific HSPG and FGF receptor expressed on the cell surface. The specific core protein of HSPG, and tissue specific differences in heparin sulfate modification result in altered bFGF regulation.

大鼠离体结肠炎模型的制作

目的:探讨一种新的结肠炎模型的制作方法.方法:处死大鼠后,离体大鼠结肠粘膜固定于Ussing槽中,温度保持(37±0.5) ℃,给予100%氧气,粘膜面孵育在Ringer液稀释的4%乙酸中,液体中给予自体血;浆膜面孵育于Ringer液中,分别孵育5 min,10 min, 15 min, 20 min.病理切片,显微镜下观察其病理变化,同时现察其损伤指数.结果:孵育5 min组可见粘膜稍变薄,孵育10 min组可见粘膜糜烂,孵育15 min、20 min组可见粘膜明显变薄,糜烂.显微镜下观察,孵育5 min组可见粘膜轻微溃疡;孵育10 min组可见粘膜溃疡,中性粒细胞浸润,粘膜及粘膜下层血管扩张充血和水肿;孵育15 min、 20 min组可见粘膜损伤严重,粘膜上皮大部缺失.结论:大鼠离体结肠在Ussing槽中,4%乙酸孵育10 min,给予自体血,底部给纯氧,(37±0.5)℃,可以初步作为一种新的结肠炎的制作方法.

2002年中国病理生理学会学术活动安排