首页 > 文献资料
-
17β雌二醇对大鼠骨髓基质干细胞成骨分化初期细胞骨架的影响
-
Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, we investigated the effect of arsenic on agonist-induced vasorelaxation and vasoconstriction using the isolated rat aortic rings in in vitro organ bath system.
-
绿茶多酚对晚期氧化蛋白产物诱导大鼠血管平滑肌细胞增殖的影响
血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖和表型改变是动脉粥样硬化的主要病理基础.研究表明,晚期氧化蛋白产物(advanced oxidation protein products,AOPP)参与了糖尿病及动脉粥样硬化等疾病的发生发展过程.本研究观察了绿茶多酚对AOPP诱导的大鼠血管平滑肌细胞增殖的影响.
-
阿米洛利对肝星状细胞增殖及分泌细胞外基质的影响
阿米洛利(amilofide)为Na+/H+交换泵(NHE)阻滞剂,是一种临床常用的保钾利尿药,近年来发现NHE与细胞的增殖、分化和凋亡密切相关.本实验应用HSC-T6细胞系,观察阿米洛利对HSC增殖及细胞外基质分泌的影响,以探索临床抗肝纤维化的新途径.
-
淫羊藿黄酮苷及其代谢产物调控骨代谢的体外实验研究
为探讨淫羊藿黄酮苷及其代谢产物对骨形成和骨吸收的调控作用,利用酶水解法和酸水解法分别制备了淫羊藿黄酮苷的2种代谢产物.采用鼠颅盖骨机械法分离和体外培养获得成骨细胞, 采用新生兔长干骨机械分离法获得破骨细胞,采用VD3诱导鼠骨髓的造血干细胞分化成破骨样的多核巨细胞.实验组培养液为10%胎牛血清+α MEM培养液+不同浓度淫羊藿黄酮苷及代谢产物,对照组不加药.分别用四甲基偶氮唑盐和3H-TdR法检测成骨细胞的增生和碱性磷酸酶,用骨钙素衡量成骨细胞的分化状态,用Actin-ring 染色观察破骨细胞形态变化,用图像分析仪分析体外骨吸收陷窝面积,用抗酒石酸酸性磷酸酶染色观察OCL的形成,用RT-PCR法检测和破骨样细胞形成有关的分子机制.发现:淫羊藿黄酮苷及其代谢产物对大鼠成骨细胞的增生有明显的刺激作用,并能够促进成骨细胞合成分泌碱性磷酸酶, 但对成骨细胞晚期分化指标骨钙素无显著作用.同时,使破骨细胞Actin-ring回缩,从而使骨吸收陷窝面积减少.此外,淫羊藿黄酮苷及其代谢产物通过影响成骨细胞中RANKL和OPG的表达而达到抑制破骨样细胞形成.以上结果说明,淫羊藿黄酮苷及其代谢产物一方面抑制破骨样细胞形成和成熟的破骨细胞对骨基质的吸收,另一方面还能够通过刺激骨形成来调控骨代谢,其中刺激骨形成的效果较强.由此推断淫羊藿黄酮苷可能是淫羊藿抗骨质疏松作用的主要有效成分.
-
It has been demonstrated that patients with asthma have a large number of NK cells and show a stronger NK activity. These results indicate that NK cell activity may be related to total IgE level in serum in healthy subjects. Previously,we have found that sodium butyrate (NaBu) markedly enhanced the IL-4-induced IgE production in the LPS-stimulated murine splenocytes in vitro, and inductive rat IgE production in vivo, and enhanced the NK cell activity ex vivo .We hypothesized that the IgE production might be involved in butyrate-enhanced NK cell activity in vivo. Mice were intraperitoneally treated/immunized with NaBu or/and Ascaris suum extract (ASC),and the spleen NK cell activity was evaluated. Furthermore, the effect of serum (NAS) on IL-2-or IFN-γ -induced spleen NK cell activity was determined. The spleen NK cell activity and IL-2-or IFN-γ -induced spleen NK cell activity of mice treated/immunized with NaBu or/and ASC were stronger than those of untreated/unimmunized mice. Although IL-4 blocked IL-2 (100 U/ml)-or IFN-γ (100 U/ml)-induced increase in NK cell activity,these NK cell activities in mice treated/immunized with NaBu/ASC were not inhibited. IgE production showed a tendency to rise in NaBu-treated mice serum, and a synergistic effect was observed with treatment of NaBu and ASC. Moreover, the NAS significantly increased IL-2(25 U/ml)-or IFN-γ (25 U/ml)-induced NK cell activity, and its effect was inhibited by anti-mouse IgE mAb.
-
Objective. To evaluate the Plasmodium falciparum CTL epitope vaccines in HLA class I allele specific human cell lines that have high frequency among Chinese population. Methods. Synthesized oligonucleotides encoding for P.f. CTL epitope genes, constructed eukaryotic expression plasmids, transfected the minigenes into HLA class I allele specific human cell lines and identified endogenous expressing of the minigenes by RT-PCR and HLA stabilization assay. Results. Two mini-genes encoding Plasmodium falciparum CTL epitopes were designed and cloned, respectively, into an eukaryotic expressing vector to form TR26 which was restricted to HLA-B51, SH6 which was restricted to HLA-A2.1, and TS, which had the two aforementioned mini-genes fused in tandem. All of these CTL epitope genes were transfected and endogenously expressed in respective cell lines containing appropriate HLA molecules. The obviously increased expressions of HLA class I molecules were detected in the transfected cell lines. It was demonstrated that the two discrete Plasmodium falciparum epitope genes were effectively processed and presented, and the close proximity of the two epitope genes in one chain as in mini-gene TS did not interfere with the processing and presenting of each epitope gene in corresponding cell line. Conclusion. A successful expression and presentation of multiple CTL epitope mini-gene in MHC class I allele specific human cell lines were demonstrated by an in vitro assay, which could be corresponding to the vaccination of CTL vaccines in people with different MHC I molecules. This work also suggested the possibility of constructing a multiple CTL epitope plasmodium falciparum DNA vaccine that could cover most of Chinese population.
-
Objective To study the inhibitory effect of oxymatrine on hepatitis B virus (HBV) in vitro.Methods Microparticle enzyme immunoassay, bDNA signal amplification assay was used for determining secreted HBsAg/HBeAg in the culture medium, HBV DNA from core particles in the cytoplasm and MTT colorimetric assay was used to assay the oxymatrine cytotoxity.Results The inhibitory rates of HBsAg and HBeAg were 40.57% and 48.27% by oxymatrine at the concentration of 2000*!μg/ml. At 100-2000*!μg/ml, it can remarkably decrease the level of viral core associated-HBV DNA in the cytoplasm. No significant toxicity was shown in such concentrations.Conclusion Oxymatrine has a potential anti-HBV activity in vitro.
-
膝关节单髁置换中聚乙烯垫片的损坏——体内植入和体外模拟的对比研究
改进的膝关节磨损模拟方法是使膝关节单髁置换(unicondylar knee replacements,UKR)与合成股骨(具有人体股骨相似的形态和材料特性)之间形成"解剖式连接".将体外标准模拟和改进模拟的UKR聚乙烯垫片与体内取出的UKR聚乙烯垫片进行损坏模式的对比,以此评估模拟改进的效果.共评价了3组UKR垫片:一组为患者体内取出的垫片(植入组,n=17);另一组是将股骨部件连接于标准金属块上进行膝关节磨损模拟之后的垫片(标准组,n=6);后一组是将股骨部件连接于合成股骨上进行膝关节磨损模拟之后的垫片(解剖组,n=6).所有UKR都具有相似的低匹配度的关节面.使用数字图像摄影测量法对关节的磨损(种类、频率和面积)进行量化.虽然这三组之间存在一些共同的磨损方式,但是标准组和解剖组垫片磨损的面积与植入组不同.三组垫片在金属股骨关节面和聚乙烯垫片接触的地方,都具有相对聚集的磨损.但只有植入组垫片存在(因磨蚀方式而产生的)相对分散的磨损.合成股骨的使用增加了磨损模拟的复杂性,但是并没产生充分接近于取出垫片的磨损模式.
-
动态观察黄芪对人巨细胞病毒感染的粒-单系祖细胞体外增殖的影响
目的:动态研究(CFU-GM)体外增殖的影响.方法:隔日计HCMV AD169体外持续感染后1~19天内黄芪注射液对CFU-GM细胞簇、集落、大集落及细胞总数变化规律.结果:培养后各组CFU-GM增殖均有不同程度的增加,7天达峰后减少.病毒组CFU-GM增殖均较空白组有明显降低(P<0.05);黄芪组、更昔洛韦组和空白组细胞增殖明显高于病毒组(P<0.01);更昔洛韦组和黄芪组CFU-GM增殖显著高于病毒组(P<0.05).结论:黄芪注射液可以促进体外培养HCMV感染CFU-GM的增殖.黄芪注射液;粒--单系祖细胞;人巨细胞病毒;细胞增殖.
-
造血干细胞体外扩增的研究进展
造血干细胞数量限制了其临床应用.随着各种造血调控因子的问世、细胞分选技术的进步及体外扩增技术的不断改进和完善,有望通过体外扩增技术来解决成人HSC移植的数量问题.造血干细胞体外扩增主要因素包括细胞园子调控造血,滋养层细胞支持造血,三维空间立体培养优化造血等影响因素.
-
骨髓基质干细胞体外诱导分化为施旺细胞的研究进展
骨髓基质干细胞是一种具有多向分化潜能的细胞,除了分化为间充质细胞谱系外,还具有向非间充质细胞谱系如神经细胞分化的潜能,在体外适宜的条件下可以诱导分化为施旺细胞.多种抗氧化剂可以诱导骨髓基质干细胞分化为施旺细胞,分化所需时间较短;多种生长因子亦有同样的作用,诱导的施旺细胞传代时寿命延长;抗氧化剂和生长因子合用,分化施旺细胞的数量更多,传代时寿命更长.
-
间充质干细胞诱导分化为血管内皮细胞的研究进展
间充质干细胞(Mesenchymal stem cells,MSCs)是一种起源于中胚层的成体干细胞,可以向所有中胚层来源的细胞及部分外胚层来源的细胞分化,且MSCs具有来源广泛、易于体外分离培养、免疫原性低,移植后无免疫排斥反应和易于基因转染等特点.血管内皮细胞属于中胚层细胞,MSCs可定向诱导分化为血管内皮细胞,在体外构建成理想的血管移植物,用于心脏组织工程瓣、血管组织工程以及再生医学领域.本文就间充质干细胞的生物学特性及诱导分化为内皮细胞的相关研究以及研究中存在的问题作一综述.
-
The antagonistic activity of one B.subtilis mutant named BS9920 on three strains of common pathogens was investigated.The assay of three consecutive days showaed that this mutant could significantly inhibit the growth of three kinds of commom infectious pathogens-Staph-lococcus.aureus.Pseudomonas aeruginosa and Pathogenic Escherichia coli and superior to the capability of BS224.This study indicated that BS-9920 possessed strong antagonistic effect in vitro.
-
牙本质及成牙本质细胞体外培养的研究现状
龋病是造成牙体缺损与缺失的主要原因,是口腔科常见的多发病,也是严重危害人类身体健康的三大疾病之一.世界卫生组织已将龋病列为危害人类健康的三大疾病之一.
-
间歇性机械拉伸对体外培养兔关节软骨细胞微管蛋白-β取向的影响
目的 探讨间歇性机械拉伸对兔关节软骨细胞生长和细胞形态及骨架的影响,为软骨细胞内力学信号传导研究的提供参考.方法 采用FX-4000TM柔性基底拉伸系统对体外单层原代培养的兔软骨细胞实施正弦波、0.5 Hz、0~5%间歇性周期应变加载,每日拉伸3h,共加载3天.对照组静态培养.3天后检测细胞活力、基质合成情况以及微管蛋白-β细胞免疫荧光染色,并对细胞周期进行检测.结果 加载组软骨细胞生长良好,氨基多聚糖和蛋白多糖的合成与对照组无明显差异;但细胞增殖指数显著增高(P<0.05);微管蛋白-β沿细胞长轴发生重排,细胞形态和取向趋于一致.结论 间歇性机械拉伸对细胞生长起到积极的作用,并且通过微管蛋白-β的重排影响细胞形态和取向.
-
Development of A Flow Chamber for Studying Cell Behaviour under Pulsatile Flow
Introduction Because of the complexity and sophistication of the in vivo environment, to systematically study the behavior of cellular response to mechanical forces has mainly relied on the use of in vitro preparations. The parallel plate flow chamber is the most common used for applying fluid shear stress to cultured cells. Most studies used parallel plate flow chambers under steady flow, and only a limited number of in vitro studies concerned with the influence of pulsatile flow. Several kinds of flow chamber were developed which can provide pulsatile flow to cells ( Helmilinger et al , 1991; Ruel et al. , 1995 ;Tacobes et al. , 1998).
-
Development of a Flow Chamber for Studying Cell Behaviour under Pulsatile Flow
Introduction Because of the complexity and sophistication of the in vivo enviromnent, to systematically study the behavior of cellular response to mechanical forces has mainly relied on the use of in vitro preparations. The parallel plate flow chamber is the most common used for applying fluid shear stress to cultured cells. Most studies used parallel plate flow chambers under steady flow, and only a limited number of in vitro studies concerned with the influence of pulsatile flow. Several kinds of flow chamber were developed which can provide pulsatile flow to cells ( Helmilinger et al , 1991; Ruel et al. , 1995 ;Tacobes et al. , 1998). If the flow in flow chamber similar to the in vivo arterial pulsatile flow can be applied to cultured cells, then more useful information may be achieved.
-
Novel Hollow Fibre Bioreactors for Growing Bulky Bone Tissue
Limitations in the size and amount of bone tissue produced in vitro via tissue engineering are mainly due to the lack of nutrient and oxygen supply to the cells some distance away from the surface. Our laboratory has investigated possibilities for growing bulky bone tissue using novel hollow fibre bioreactors. The hollow fibre membranes embedded in the three dimensional scaffold are porous and semi permeable, which can serve the function of arteries and veins.
-
用体外心内膜赘生物模型评价万古霉素和达托霉素对甲氧西林耐药金葡菌和异质性万古霉素中介金葡菌的抗菌活性
糖肽类抗生素是治疗甲氧西林耐药金葡菌(MRSA)感染的传统药物之一.然而,在持续抗生素的选择压力之下,MRSA和异质性万古霉素中介金葡菌(hVISA)等不敏感菌株逐渐涌现.hVISA感染可导致预后差,甚至万古霉素治疗失败.本研究采用心内膜赘生物的PK/PD模型评估万古霉素和达托霉素对万古霉素敏感MRSA和hVISA的抗菌活性.