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Liver fibrosis is a gradual process of increased secretion and decreased degradation of extracellular materials (ECM). Most author hold that the process is initiated by the damage of hepatic cells (HCs), which leads to activation and secretion of multiple cellular factors of Kupffer cells. These factors, along with the cellular factors secreted by damaged HCs, thrombocytes and endothelial cells of the hepatic sinusoid, and some chemical mediators are activators of hepatic stellate cells (HSCs). Being activated, HSCs differentiate into myofibroblasts, and, via self-secretion and parasecretion, proliferate and synthesize a massive amount of extracellular materials which gradually accumulate and lead to formation of liver fibrosis.1 Since fibrosis is a common course in a variety of chronic liver diseases, prevention against its formation is of great importance. The following are the recent achievements of traditional Chinese medicine (TCM) in this field.
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丹参酸乙的抗氧化作用对大鼠肝星状细胞增生的影响
目的:研究丹参酸乙(salvianolic acid B,SAB)的抗氧化作用对大鼠肝星状细胞(hepatic stallate cells,HSC)增生周期的影响.方法:采用原位灌注法消化大鼠肝脏,108g@L-1nycodenz密度梯度离心,分离肝星状细胞,分别以不同浓度的SAB或MDA/SAB处理细胞,3H-TdR掺入法观察细胞的增生能力,流式细胞术分析细胞周期各时相变化及凋亡情况.结果:1和10 tanol@L1SAB可显著抑制HSC的增生(2862±123M&2988±407 vs 4986±727,P<0.05,P<0.01),这种作用主要是抑制了细胞周期过程中G期向S期的过渡;1和10 pmol@L1SAB均可抑制MDA刺激的HSC增生(3067±73l&3042±321vs4007±587,P<0.05,P<0.01);两组剂量的SAB均不促进HSC的凋亡.结论:丹参酸乙可抑制体外培养HSC的增生,这种抑制作用与SAB的抗氧化作用有一定的关系.
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阿米洛利对肝星状细胞增殖及分泌细胞外基质的影响
阿米洛利(amilofide)为Na+/H+交换泵(NHE)阻滞剂,是一种临床常用的保钾利尿药,近年来发现NHE与细胞的增殖、分化和凋亡密切相关.本实验应用HSC-T6细胞系,观察阿米洛利对HSC增殖及细胞外基质分泌的影响,以探索临床抗肝纤维化的新途径.