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冻干鼠表皮生长因子治疗慢性溃疡创面的观察和护理
外伤、烧伤等后期创面长时间不愈合,形成慢性溃疡创面,部位大多位于下肢和骶尾部.创面长时间不愈合,不仅给患者精神和生活上带来许多痛苦和不便,而且还影响患者生活质量.我们用冻干鼠表皮生长因子(Mouse Epidermal Growth Factor简称mEGF),治疗慢性溃疡计10个创面取得了满意的效果,总结如下.
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宫内急性缺血缺氧后胎鼠肾脏表皮生长因子及其受体表达变化的研究
研究证实,外源性表皮生长因子(EGF)对防治缺血缺氧性肾损伤有重要作用.同时,对胚胎肾发育的研究表明,转化生长因子-α(TGF-α)对肾脏的发育和成熟起主要促进作用[1].EGF和TGF-α共同作用于一个受体--表皮生长因子受体(EGFR).研究肾组织EGF和TGF-α及其受体EGFR在宫内窘迫时的动态变化,无疑对指导临床应用EGF防治围生期缺血缺氧性肾损伤具有重要的指导意义.
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Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, micro-tubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the su-pernatant were signiifcantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mes-enchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesen-chymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.
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Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici-ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic ifbroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was signiifcantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.
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Previous studies have shown that somatic sensation by acupuncture and visceral nociceptive stimulation can converge in the nucleus tractus solitarii where neurons integrate signals impact-ing on the function of organs. To explore the role of the nucleus tractus solitarii in the protective mechanism of pre-moxibustion on gastric mucosa, nucleus tractus solitarii were damaged in rats and pre-moxibustion treatment at the Zusanli (ST36) point followed. The gastric mucosa was then damaged by the anhydrous ethanol lavage method. Morphological observations, enzyme linked immunosorbent assays, and western immunoblot analyses showed that gastric mucosa surface lesion and the infiltration of inflammatory cells were significantly ameliorated after pre-moxibustion treatment. Furthermore, the gastric mucosal damage index and somatostatin level were reduced, and epidermal growth factor content in the gastric mucosa and heat-shock protein-70 expression were increased. These results were reversed by damage to the nucleus tractus solitarii. These findings suggest that moxibustion pretreatment at the Zusanli point is protective against acute gastric mucosa injury, and nucleus tractus solitarii damage inhibits these responses. Therefore, the nucleus tractus solitarii may be an important area for regulating the signal transduction of the protective effect of pre-moxibustion on gastric mucosa.
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Objective. To evaluate the effects of epidermal growth factor (EGF) on intestinal permeability and bacterial translocation in rats with acute pancreatitis during total parenteral nutrition (TPN). Methods. Thirty-two male Sprague-Dawley rats that underwent injection of 3.5% sodium taurocholate solution into the pancreatic duct were randomly divided into one of the following two groups: (1) received only TPN (control group) or (2) received TPN with EGF at a dose of 0.2 mg· kg-1· day-1 (Egf group). On fifth day of total parenteral nutrition, samples from mesenteric lymph nodes, pancreas, liver and spleen were harvested for cultures. Water, protein and DNA content in jejunal mucosa were determined. D-xylose and fluorescein isothiocyanate (FITC)-dextran were instilled into the lumen of a ligated segament of small intestine. Thirty minutes later, superior mesenteric vein D-xylose and plasma FITC-dextran concentration were measured. Results. Positive cultures in liver and spleen, as well as FITC-dextran concentration in the Egf group were significantly lower than in the control group. Protein and DNA content in jejunal mucosa in the Egf group were significantly higher than in the control group. Conclusion. The results indicate that EGF may prevent increased intestinal permeability and bacterial translocation in rats with acute pancreatitis during TPN.
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曲妥珠单抗在乳腺癌中的耐药机制研究进展
HER2即人表皮生长因子受体-2(Human epidermal growth factor receptor-2,HER2),属于酪氨酸激酶受体家族成员,是细胞生长、分化和存活的重要调控因子.临床数据显示,20%~25%的乳腺癌患者存在HER2基因扩增或蛋白过表达.HER2的过表达预示着临床预后差、易转移及复发.曲妥珠单抗是HER2的人源化单克隆抗体,用于治疗HER2过表达的早期或转移性乳腺癌患者.然而曲妥珠单抗的单独治疗客观反应率仅12%~34%,并且多数转移性患者在使用1年之内出现耐药现象[1].为了更有效地治疗HER2过表达乳腺癌,需要详细理解曲妥珠单抗的作用及耐药机制.在此就有关曲妥珠单抗的耐药机制新进展进行阐述,为乳腺癌靶向治疗药物的基础研究和临床合理应用提供参考.
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三阴型乳腺癌的研究进展
三阴型乳腺癌(triple negative breast cancer,TNBC)指的是雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)和人类表皮生长因子受体-2 human epidermal growth factor receptor-2,Her-2)均为阴性表达的乳腺癌.TNBC的生物学特征不同于其他类型的乳腺癌,临床行为更具有侵袭性.
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表皮生长因子信号转导的复杂性与生物学效应多样性
表皮生长因子(epidermal growth factor,EGF)是1955年9月美国纳什维尔市范德比大学医学院Stanley Cohen等在试验中偶然发现的一种能直接刺激表皮生长角化的因子.随后进一步证明EGF对多种组织来源的细胞增殖有明显的促进作用.由于EGF的发现及随后的研究成果,Cohen等两位科学家成为1986年度生理学诺贝尔奖获得者,因而也使这一领域变得更加引人注目,大大促进了这一领域的研究进展.目前,不但已了解了其一般生物学特性,还完成了它的基因定位、cDNA克隆、mRNA表达、分子水平的检测以及逐步研究发现由它介导的多种生物学效应.这些不同的生物学效应与EGF细胞信号转导的复杂性密切相关.因此现就EGF介导的细胞信号转导的复杂性与其生物学效应的多样性进行阐述.
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银锌霜与重组人表皮生长因子联合治疗深Ⅱ度烧伤的疗效分析
笔者等自1999年12月至2003年12月,采用银锌霜与重组人表皮生长因子(recombinant humar epidermal growth factor, rhEGF)联合治疗深Ⅱ度烧伤患者,获得较好的效果,现将其疗效分析如下.
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大鼠内耳发育过程表皮生长因子的表达(英)
Since the two independent discoveries in 1987 indi-cated that hair cells within cochlea of bircls were capableof spontaneous regeneration, recent observations havedemonstrated that, in response to injury, mammals pos-sess a limited capacity of regenerating inner ear sensoryepithelia, but the mechanism of regeneration is still tm-known. Lewfebvre (1993) and Lambert (1994) reportedrespectively that retininoic acid (RA) and transforminggrowth factor alpha (TGF-α) could stimulate hair cell re-generation in the mammalian inner ear. These studies in-dicated that the cellular factors participating the normaldevelopment of cells could play a critical role in the re-generative process of hair cells.Epidermal growth factor (EGF) plays a critical rolein proliferation and differentiation of normal cells and tis-sues and it is extensively expressed in many mature andembryonic tissues.
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血管内皮生长因子和表皮生长因子信号通路的联合抑制在非小细胞肺癌治疗中的应用
肺癌是全球癌症相关死亡的首位原因[1],已成为人类面临的日益严重的公共卫生问题,各国政府均投入了大量的人力物力,以期在肺癌研究领域有所突破[2].
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白芨胶载EGF对伤口愈合的影响
如何促进创伤后伤口愈合、缩短愈合过程是近年来国内外相关学科的重点研究课题之一,其中EGF和白芨在伤口愈合中的作用越来越受到人们的重视并做了深入的基础实验和临床研究,现将EGF和白芨的生物学功能及其在伤口愈合中的作用综述如下.
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重组人表皮生长因子配合超脉冲C02激光磨削治疗痤疮后瘢痕127例
面部痤疮后瘢痕为常见的皮肤损容性疾病,采用激光磨削进行治疗,可取得明显效果,本文旨在探讨重组人表皮生长因子(recombinant human epidermal growth factor, rhEGF)对面部痤疮瘢痕磨削后创面愈合的作用.
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MRI 在三阴性乳腺癌中的诊断与疗效评估
乳腺癌是严重影响女性身心健康的常见恶性肿瘤,它是一类异质性明显的疾病,有各种各样的形态学表现、临床进程和对治疗的反应[1]。在临床中,通常用雌激素受体(estrogen receptor,ER)、孕激素受体(pro-gesterone receptor,PR)和人类表皮生长因子受体2(human epidermal growth factor receptor2,HER2)表达程度的免疫组化谱来识别乳腺癌不同亚组的患者。三阴性乳腺癌(tripple negative breast cancer,TNBC)是指 ER、PR 阴性,HER2无扩增的一种乳腺癌亚型, TNBC 仅占所有乳腺癌发病率的10%~20%,但由于它具有较强的侵袭性且在临床中缺乏有效的靶向治疗,所以在乳腺癌死亡率中占有较大的比例[2]。故早期发现、早期诊断、早期治疗的原则在 TNBC 中显得尤为重要。磁共振成像(MRI)是目前诊断乳腺疾病敏感的影像学检查,其在乳腺癌中的应用价值也渐渐受到肯定。本文将就 TNBC 在 MRI 中的特征及 MRI在 TNBC 新辅助化疗中的应用与价值做一综述。
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重组人表皮生长因子治疗吸入性损伤的临床观察
重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)具有促进表皮细胞生长、加速创面愈合、缩短愈合时间的作用,且无毒、无化学和物理刺激性,黏膜耐受性好.但rhEGF应用于吸入性损伤的治疗及促进气管黏膜愈合的作用尚鲜见报道.笔者应用rhEGF对本单位收治的20例吸入性损伤患者进行治疗,结果表明rhEGF对吸入性损伤受损气管黏膜的愈合有一定促进作用.
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首诊乳腺癌筛查发现未知晓糖尿病一例
1临床资料
女,54岁,因“左乳包块2周”入院。体检:左乳2点钟方向扪及一约3 cm ×2 cm包块,质硬,边界不清,活动度差,无压痛。乳腺彩超:左乳实性占位性病变,血流信号丰富,乳腺癌不能除外。左侧腋窝较大异常回声,转移性淋巴结不能除外。乳腺钼靶:左乳肿块伴多枚细小呈簇状钙化,左腋淋巴结增大,密度增高,乳腺癌可能。既往体健,否认糖尿病( diabetes mellitus ,DM)、高血压等病史。入院后行核心穿刺活检确诊左乳腺癌,免疫组化:雌激素受体( estrogen recep-tors,ER)(-)、孕激素受体(progesterone receptors,PR)(-)、人类表皮生长因子受体( human epidermal growth factor recep-tor,C-erbB-2)(++)、P5330%(+)、Ki6710%(+)。 -
乳腺癌原发灶与同期腋淋巴结转移灶雌、孕激素受体和人类表皮生长因子受体2的表达差异研究进展
乳腺癌的发生发展具有激素依赖性,癌组织中雌激素受体(estrogen receptor, ER)、孕激素受体(progesterone receptor, PR)的表达状况是评估内分泌治疗疗效和预后的重要指标。人类表皮生长因子受体2( human epidermal growth factor re-ceptor 2, HER2)在20%~30%的乳腺癌中过表达,此类患者倾向于复发、转移早且生存期短[1]。美国国家综合癌症网络(National Comprehensive Cancer Network , NCCN)指南推荐分子靶向治疗作为HER2阳性转移性乳腺癌的首选疗法[2]。目前乳腺癌术后治疗主要依据原发灶的相关生物学指标。近年来复发转移病灶中ER、PR、HER2等生物学指标与原发灶之间的表达差异越来越受重视,有必要对复发转移灶受体进行检测[6-15];同时发现腋窝淋巴结转移的乳腺癌患者,淋巴结转移灶与原发灶中ER、PR、HER2等生物学指标也存在一定差异[16-23],因此结合同期腋淋巴结转移灶及原发灶的受体检测有可能更好的评估术后复发风险、判断预后和为个体化治疗提供重要理论依据。本文对乳腺癌原发灶与同期腋淋巴结转移灶中ER、PR和HER2的表达差异及临床意义的研究进展综述如下。
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Chemotherapy, endocrine therapy and molecular targeted therapy are vital means in the treatment of metastatic breast cancer (MBC), whose reasonable and standard applications are of great importance to prolong patients’ survival and improve the quality of life. The expressions of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2) present signiifcant differences between primary and metastatic breast cancer. However, these differences may affect the selection of MBC patients for therapeutic strategies and judgment on the prognosis. Hence, the relevant researches on variations of hormone receptors and HER-2 in primary and metastatic breast cancer, discordant causes of ER, PR and HER-2 expression in primary and metastatic lesions and clinical value of biopsy to the metastases are reviewed in the study.