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负压封闭引流术联合重组牛碱性成纤维细胞生长因子对四肢电烧伤创面的疗效观察
临床上,电烧伤是指电流通过机体时,通过电热效应、电生化效应及电火花、电弧等机制导致的组织损伤,其损伤范围往往累及皮肤、皮下组织及肌肉、血管、神经等深层组织,其病理特征为:损伤常呈现外小内大形态,深层组织可呈夹心样坏死,同时存在间生态组织[1-2].这类创面的治疗较为棘手,往往需要多次清创和植皮.近年来,我院采用负压封闭引流术(vacuum sealing drainage,VSD)联合重组牛碱性成纤维细胞生长因子(recombinant bovine basic fibroblast growth factor,rb-FGF)治疗15例四肢电烧伤创面,疗效较佳,报告如下.
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碱性成纤维细胞生长因子在胃溃疡愈合中的表达及意义
目的:探讨人胃溃疡愈合过程中碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的变化及其作用.方法:采用免疫组织化学法,对正常胃黏膜(20例)、胃溃疡活动期(24例)、愈合期(26例)和瘢痕期(20例)组织的bFGF的表达进行定位观察和图像半定量分析.结果:bFGF在正常胃黏膜呈弱阳性表达,在胃溃疡急性期呈阳性表达,在愈合期和瘢痕期呈强阳性表达.阳性信号主要位于腺上皮、细胞外基质、成纤维细胞和内皮细胞中.bFGF平均光密度值和面密度值在胃溃疡活动期、愈合期、瘢痕期与正常胃黏膜相比均有显著性差异(0.247±0.042&0.321±0.096&0.296±0.048 vs 0.125±0.062,P<0.05;0.131±0.024&0.165±0.031&0.162±0.028 vs 0.081±0.008,P<0.05),在愈合期及瘢痕期组织与活动期溃疡相比也有显著性差异(0.321±0.096&0.296±0.048 vs 0.247±0.042,P<0.05;0.165±0.031&0.162±0.028 vs 0.131±0.024,P<0.05).结论:在人胃溃疡愈合过程中,存在着内源性bFGF由弱到强的演变,说明bFGF与胃溃疡愈合密切相关.临床上合理应用bFGF有助于消化性溃疡愈合.
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Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici-ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic ifbroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was signiifcantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.
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The purpose of the present study was to determine protectivie effects of basic fibroblast growth factor (bFGF) on cochlear neurons and hair cells in vitro and in vivo. In experiment I, cultured spiral ganglion neurons (SGNs) prepared from P3 mice were exposed to 20mM glutamate for 2 hours before the culture medium was replaced with fresh medium containing 0, 25, 50, and 100 ng/ml bFGF, respectively. Fourteen days later, all cultures were fixed with 4% paraformaldehyde, and stained with 1% toluidine blue. The number of surviving SGNs were counted and the length of SGNs neurites were measured. Exposure to 20 mM glutamate for 24 hours resulted in an inhibition on neurite outgrowth of SGNs and elevated cell death. Treatment of the cultures with bFGF led to promotion of neurite outgrowth and elevated number of surviving SGNs. Effects of bFGF were dose dependent with the highest potency at 100 ng/ml. In experiment Ⅱ, in vivo studies were carried out with guinea pigs in which bFGF or artificial perilymph was perfused into the cochlea to assess possible protective effects of bFGF on cochlear hair cells and compound action potentials(CAP). The CAPs were measured before, immediatly and 48 hours after exposure to noise. Significant differences in CAP were observed (p<0. 05 ) among the bFGF perfused group, control group(t =3. 896 ) and artificial perilymph perfused group (t =2. 520) at 48 hours after noise exposure, Cochleae were removed and hair cell Loss was analyzed in surface preparations prepared from all experimental animals. Acoustic trauma caused loss of 651 and 687 inner hair cells in the control and artificial perilymph perfused group, respectively. In sharp contrast, only 31 inner hair cells were lost in the bFGF perfused ears. Similarly, more outer hair cells died in the control and perilymph perfuesed group (41830 and 41968, respectively) than in the group treated with bFGF (34258). Our results demonstrate that bFGF protected SGNs against glutmate neurotoxicity in vitro. In addition, treatment with bFGF also protected hair cells from acoustic trauma.
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神经生长因子和碱性成纤维细胞生长因子联用治疗坐骨神经损伤实验研究
AIM:To explore the co- effect of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) on the sciatic nerve after injury. METHODS: 96 rats were divided into normal saline group, NGF group, bFGF group and NGF+ bFGF group. Sciatic nerve function index (SFI), nerve conduction velocity, regeneration axon counting were detected in the 3 rd, 6 th, 9 th, 12 th week postinjury. RESULTS:The end of SFI, nerve conduction velocity, regeneration axon counting in NGF+ bFGF group were higher than those in the other groups. CONCLUSION:The co- effect of NGF and bFGF on sciatic nerve is better than the effect of NGF or bFGF alone.
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医用生物蛋白胶对注射型骨形态发生蛋白成骨活性的影响
Objective To observe influence of fibrin sealant(FS) on osteoinductive ability of inject type BMP.Method The inject type BMP power was dissolved in the main glue part or thrombin part of FS, then mixed with the main glue part or thrombin part of FS into gel, observe coagulating time, then implant composite into the thigh muscle pouch of mice to evaluate their capacity to induce new bone formation, and compared to the single BMP implant group.Result There was no difference in the coagulating time between two mixing method, the osteoinductive ability of implants BMP dissolved in the main glue part or thrombin part of FS group was higher than that of simply BMP implant group.Conclusion FS was perfect carrier to inject type BMP.
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碱性成纤维细胞生长因子与乙型肝炎致纤维化的相关性分析
Background: Now, many researcher are interested in expression of basic fibroblast growth factor(bFGF) in hepatic tissue. Nevertheless,hepatic tissue of experimental animals and liver cancer were employed.Studies about expression of bFGF and mRNA bFGF in hepatic tissues are now unavailable.
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Increased glycated basic fibroblast growth factor in diabetic skin reduces the cell viability and angiogenesis of human dermal microvascular endothelial cells
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兔脑出血灶周围脑组织bFGF,VEGF及TNF-α的表达
Intracerebral hemorrhage frequently occurs in nervous system diseases, and le ads to secondary cerebral lesion, such as ischemia, edema of perihematomal brain r egions and the biochemical change of the cell. Recent studies have confirmed tha t cytokines such as basic fibroblast growth factor(bFGF), vascular endothelial g r owth factor(VEGF) and tumor necrosis factor(TNF-α) are involved in the pathoge n esis of cerebral hemorrhage. In nervous system, positive expressions of bFGF and TNF-α are in neurons and astrocytes, and that of VEGF is in vascula r endothelial cells. The content of bFGF protein in brain regions is different a t different time after brain injuries. There was no literature about detectin g the expressions of bFGF, VEGF and TNF-α protein in the perihematomal brain r egion s after intracerebral hemorrhage. Using immunohistochemical technique,the author detected the expressions of bFGF,VEGF and TNF-α in the perihematomal brain re g ions in order to research the pathological mechanism of intracerebral hemorrhage.
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腹膜透析对碱性成纤维生长因子在大鼠腹膜表达的影响
腹膜超滤衰竭(UFF)是长期腹膜透析(PD)的主要并发症,而UFF患者腹膜组织病理检查显示新生血管明显增多.碱性成纤维生长因子(bFGF)是目前公认的促血管生成因子,在腹膜间皮细胞呈结构件表达.在CAPD患者腹透液中也可榆测到bFGF[1].因此,本研究探讨bFGF在腹膜组织新牛血管形成中可能的作用及机制.
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血管内皮生长因子在白血病发病中的研究进展
研究发现,白血病与其他实体瘤一样存在着显著的新生血管形成,影响血管形成的因素在调节血管形成中发挥较为重要的作用,这些血管生长因子同样作用于白血病的发生发展及其转归过程中.血管生长因子包括血管内皮生长因子(vascular endothelial cell growth factor,VEGF),碱性成纤维细胞生长因子(bovine basic fibroblast growth factor,bFGF),肿瘤生长因子等,而VEGF是促进血管生成的关键因子,也是白血病生长、转移和存活的重要因子[1].
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碱性成纤维细胞生长因子及地塞米松防治皮瓣缺血坏死的研究现状与进展
皮瓣的转移和移植在组织缺损修复和功能重建上发挥着不可替代的重要作用,是修复重建外科和整形再造外科常运用的治疗手段之一.多年来尽管皮瓣的设计和手术技巧在不断改善,但皮瓣缺血乃至坏死始终是一个常见的临床问题.
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碱性成纤维细胞生长因子缓释微球促周围神经再生作用的研究进展
碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)是一种具有广泛生物学活性的肽类生长因子,对神经外胚层和中胚层的多种细胞具有促增殖和分化作用,在神经创伤修复这一研究领域中具有广阔的应用前景.但bFGF对热和酸均不稳定,在体内的半衰期为3~5 min,全身应用则会被快速灭活而难以发挥其有效作用,局部穿刺给药对深部神经损伤难以保证每次释药部位的准确性,且不适宜长期用药[1,2].因此,研究bFGF的缓释制剂对长期维持损伤局部的有效药物浓度、促进神经再生有着重要意义.现就有关bFGF促周围神经再生以及bFGF缓释制剂的研究综述如下.
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Objective To study the biological effects of basic fibroblast growth factor (bFGF) in the process of embryo implantation in mouse Materials & Methods The transcription and translocation of bFGF and its receptor (Flg) in endometrium of 60 Kunming mice were detected with the methods of im munohistochemistry and hybridization in situ. Endometrial tissue was obtained on the day 4 from pregnancy mice. The cells were cultured and attached in 1 : 1 F12/DMEM (vol/vol) supplemented with 10% FBS for 24 h The medium supplemented with 1% FBS and bFGF (50 ng/mL), anti-bFGF antibody (250 ng/mL) or anti-Flg antibody (250 ng/mL) was added in different culture wells. At different culture stages, the biological effect of bFGF on cell survival and expression of LN, FN and c-myc was detected by using MTT analysis, immunohistochemistry and hybridization in situ.Results bFGF and Flg was located in luminal and glandual cells on day 4 and 5 of pregnancy. Embryonic implantation was accompanied by increased bFGF and its recep tor in decidual cell around implantation site, in which low level of bFGF and its recep tor was apparent on day 7 and 8 of pregnancy. In vitro, the OD value in culture wells containing bFGF was significantly higher than that in control group. Exogenous bFGF promoted the expression of LN, FN and c-myc.Conclusion Changes in the cell-specific distribution of bFGF and the effects of bFGF on cultured endometrial cells imply a multifunctional role of the growth factor in uterine cell proliferation, differentiation and embryonic implantation.