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对实验动物行创面细菌定量检测的经验体会
感染是影响创伤修复的重要因素,是现代战争创伤救治中迫切需要解决的重大问题之一[1].感染的发生发展取决于两个方面,一是机体防御功能;二是致病菌的毒力、侵袭力和细菌量.创面组织细菌定量检测可准确地反映创面组织内的细菌含量,是判断不同抗菌药物和不同防治方法对创伤感染防治效果的重要而基本的方法,对于早期诊断和预防侵袭性感染也是有效而实用的[2].通过细菌定量检测,可为创伤机体的清创时机、方法及抗生索的应用提供科学的依据.然而在细菌定量检测过程中,每一步骤均需要有高度的主观判断能力,这与个人的技术经验及细菌学基础知识水平密切相关.因此,对于伤口护理工作鼢者,特别对进行创面愈合实验研究的护理工作者来说,细菌定量检测是一项陌生而难度较大的技术.如何提高检测结果的准确性,增加实验的信度,是实验者需要重点解决的问题.笔者通过对24只创伤大鼠进行创面细菌定量检测,总结出几点经验与体会,以供探讨.
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碱性成纤维细胞生长因子与乙型肝炎致纤维化的相关性分析
Background: Now, many researcher are interested in expression of basic fibroblast growth factor(bFGF) in hepatic tissue. Nevertheless,hepatic tissue of experimental animals and liver cancer were employed.Studies about expression of bFGF and mRNA bFGF in hepatic tissues are now unavailable.
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微基因修饰雪旺氏细胞移植对脊髓损伤中枢传导功能的作用
Objective To approach the effect of microgene pSVPoMcat to modify genetically Schwann cells (SC) on central conduction functionafter spinal cord injury (SCI) . Method Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically modify SC (group A), SC implanted group (group B), and the control group (group C). The cortical somatasensory evoked potentials (CESP) and combined behavioral score (CBS) were continuous monitored from operation second week to twelfth week Result The result showed that the peak latency and peak amplitudes of group A, B have a recovery tendency and it was constant with CBS. Conclusion We concluded that the microgene pSVPoMcat to modify genetically Sc may play a promotion role in recovery of central conduction function after SCI.
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日本血吸虫模拟虫卵抗原的初步研究
The egg of Schistosoma japonicum is a chief pathogenic factor. The preparation of egg antigen is a troublesome work, and needs a large number of experimental animals. In order to explore and develop an egg mimotope that can be used in the study of diagnostic reagents and vaccines, we employed the phage display random 15-peptides library to mimic the antigen epitope recognized by mAb 6B12 which is specific to egg antigen of Schistosoma japonicum.
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硅油预防肠粘连的实验研究与临床应用
目前,尚无一种能完全防止肠粘连的可靠方法.我们从1983年7月至1985年3月,用二甲基硅油(简称硅油)进行了防止腹膜粘连的实验,并应用于临床,防治粘连性肠梗阻获得了较满意的效果.
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幼犬食管再生的研究
本组实验是取发育成长中的幼犬进行食管再生研究,目的为小儿食管重建探索一条新的途径.现将实验结果报道如下:
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高热治疗动物移植性肿瘤试验初步报告
自从Busch W(1866年)报导了2例经组织学证明了的面部恶性肿瘤,因偶患丹毒发高热,导致肿瘤消退后,逐渐引起了学者们的注意.