首页 > 文献资料
-
不同脂肪酸饮食对大鼠肝脏磷酸化eIF2α的影响
内质网应激(ERS)是指细胞内质网生理功能发生紊乱的一种亚细胞器病理状态.近年来众多证据表明,ERS与胰岛素抵抗(IR)密切相关.本研究通过观察不同类型的脂肪酸对p-eIF2α有无影响,进一步阐明ERS是否参与了脂肪酸饮食对胰岛素敏感性的影响机制.
-
L-精氨酸对酒精性肝脂肪变大鼠肝组织NOS表达及氧应激的影响
目的:探讨L-精氨酸对酒精性肝脂肪变大鼠肝组织NOS表达及氧应激的影响.方法:采用在饮水中力A酒精的方法建立酒精性肝脂肪变动物模型.32只SD大鼠随机分成4组,每组8只.A,B组分别喂饲400 mL/L乙醇至16,20 wk;C组喂饲乙醇同B组,自17 wk起腹腔注射L-精氨酸;D组为正常对照组.B,D组自17 wk起腹腔注射等量的生理盐水.应用免疫组化和逆转录多聚酶链反应(RT-PCR)的方法检测肝组织中NOS的蛋白和mRNA表达,同时测定肝组织中NO,MDA,GSH,SOD含量,并观察肝组织病理变化.结果:A,B组肝组织出现了不同程度脂肪变性,B组更为显著(t=76.5,P<0.05).与D组相比,A,B组肝组织中NO,MDA含量、iNOS表达明显增高(P<0.01);GSH,SOD含量、eNOS表达明显降低(P<0.05).与B组相比,C组肝组织脂肪变性被逆转或明显减轻(t=62.5,P<0.05),NO含量无显著改变,MDA含量、iNOS表达明显降低(P<0.05);GSH,SOD含量及eNOS表达明显升高(P<0.05).结论:L-精氨酸对酒精性肝脂肪变的治疗作用可能与iNOS表达降低、eNOS表达升高以及氧应激减轻有关.
-
正常与硬化肝组织基因表达差异的初步分析
目的:了解肝硬化的基因表达概况,寻找在肝硬化及正常肝组织中差异表达基因.方法:以各24例肝硬化及正常肝组织的总RNA混合定量后反转录合成含有α-32P dATP的cDNA为探针,与Atlas微阵列表达分析膜杂交.结果:放射自显影结果经AtlasImageTM软件分析显示:在所分析的588种已知基因中,Integrin beta 7、collagen type XVⅢ等17个基因在肝硬化组织中表达上调,TFDP2、BAK、ABL等98个表达下调,参与细胞增生、凋亡、分化、细胞间相互作用、与细胞侵袭相关的基因表达水平发生了明显改变.结论:通过Atlas微阵列系统分析发现的这些基因的表达改变组成了一个肝硬化特异的基因表达谱.系统地研究肝硬化的基因表达改变,与肝硬化发生相关基因的差异变化可为肝硬化诊断和治疗提供线索.
-
基质金属蛋白酶在非酒精性脂肪性肝炎患者肝组织中的表达
目的:探讨MMP-1,NMP-2,MT1-MMP和MT2-MMP在NASH肝纤维化中的相互作用及可能机制.方法:采用原位分子杂交方法检测40例NASH患者的肝穿刺标本中MMP-1,MMP-2,MT1-MMP和MT 2-MMPmRNA的表达.结果:MMP-1,MMP-2,MT1-MMP和MT 2-MMPmRNA主要表达在纤维间隔和汇管区的间质细胞(肝星状细胞等),且MMP-2和MT1-MMP的表达细胞有重叠.NASH组MMP-2,MT1-MMP,MT 2-MMP mRNA的表达均低于ASH组(P=0.03,P=0.007,P=0.001)结论:MMP-1,MMP-2,MT1-MMP和MT 2-MMP在NASH肝纤维化的发生和发展中可能起重要作用;MMP-2和MT1-MMP在基质降解过程中可能有协同作用;肝星状细胞为肝组织内MMP-1,MMp-2,MT1-MMP和MT2-MMP的主要产生细胞.
-
Objective: To elucidate the expression and significance of cell cycle protein cyclin D1 in human hepatocellular carcinoma (HCC). Methods: The expression of cyclin D1 protein in 29 cases of HCC tissues was detected by immunohistochemical ABC method, and the relationship between its positive rate and pathological grades of HCC tissues was analyzed. Results: The positive rate of cyclin D1 in HCC tissues was 58.6%(17 in 29 cases), whereas only 18.2% (2 cases of 11 cases) in the non-tumor liver tissues immediately adjacent to HCC tissues (LAH). There was significant difference between grade II and LAH tissues (P<0.05), and between grade I and grade III on the positive rate of cyclin D1 (P<0.05), respectively. Conclusion: Cyclin D1 may be regarded as an oncogenic marker during the genesis and development of HCC, and its role in the transforming process from G1 phase to S phase of HCC cells needs further studies.
-
PURPOSE. To investigate the sensitivity, specificity, and spatial distribution of the product of p28 gene (p28(GANK) protein) in human hepatocellular carcinoma (HCC) and nonhepatocellular carcinomas in relation to immunostaining with Cytokeratin 18 (CK18), alpha-fetoprotein (AFP), and Hepatocyte paraffin 1 (HepPar1). METHOD. In this retrospective study, formalin-fixed paraffin-embedded tissues from 24 HCCs, five intrahepatic cholangiocarcinomas (ICC), five combined hepatocellular cholangiocarcinomas (C-HCC-CC) and mine metastatic hepatic carcinomas (MHC) were immunostained for p28(GANK) as well as CK18, AFP and HepPar1. Only cases with more intensified staining in carcinoma contrast to the adjacent liver tissues were accepted as positive. RESULT. In HCC, p28(GANK) was expressed restrictively in hepatocytes of both para-lesion and carcinoma liver tissues, while absent in the bile duct epithelial cells, Kupffer cells, and other interstitial cells. The positive staining of p28(GANK) was noted in 16 (66.7%) specimens of HCC and three (60.0%) specimens of C-HCC-CC, and no specific lesion staining was found in all tested specimens of ICC and MHC. Sensitivity and specificity for hepatocyte-originated carcinoma were, respectively, 65.5% and 100% for p28(GANK), 79.3% and 85.2% for CK18, 20.7% and 100% for AFP, 79.3% and 92.0% for HepPar1. CONCLUSION. The hepatocytic staining for p28(GANK) is clearly useful in differentiating hepatocyte-originated carcinoma from non-HCC. p28(GANK) may be used as an ancillary marker for the diagnosis of HCC.