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不同功率密度氦氖激光照射对培养瘢痕成纤维细胞生长的影响
Objective To explore the relationship between power density of He-Ne laser with the growth of fibroblasts in hypertrophic scar(HS).Methods The cultured fibroblasts in HS were irradiated with He-Ne laser(Wavelenth 632.8 nm),various power densities such as 50mW/cm2,100mW/cm2 and 150mW/cm2 were respectively adopted once a day for 10 minutes.After 1,3 and 5 times of He-Ne laser for dose rate irradiation separatedly,the cell count and cell circle analysis were counted and examined by trypan blue staining and flow cytometry .Results The amount of cell after 1 times of 50mW/cm2 laser irradiation was markedly more than the control(P<0.01).The amount of cells after 5 times of 100mW/cm2,3 and 5 times of 150 mW/cm2 laser irradiation was less than the controls(P< 0.05).The result of cell circle analysis was corresponded with that of the cell count.Conclusion Both stimualtion and inhibition of He-Ne laser on the growth of scar fibroblasts can be obtained with various power densities.Power density of He-Ne laser associates with the effects on the growth of scar fibroblasts.
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兔耳增生性瘢痕的形态学观察与羟脯氨酸含量变化
Objective To establish animal model for hypertreophic scar and study the characteristics of its morphology and collagen metabolism.Methods A total of 64 wounds(diameter of 6 mm each ) with total skin loss were made on the ventral side of rabbit ear using a trephine. Morphology and collagen metabolism of scar wound were studied at 14,21,35,70 and 98 days after operation,respectively.Results There were 76% elevated scars developed (45/59 wounds) on the ventral side of rabbit ear on 21st and 35 days.The number of fibroblast decreased,but irregular-arranged fibers still presented in the elevated scars at 70 and 98 days after operation. Hydroxyproline content in elevated scars at 21 days was higher than that in normal skin(P< 0.05),and at 35 days was 3 times as that in normal skin and at 98 days was also markedly higher than that in normal skin(P< 0.05).Conclusion Excessive deposition of collagen is a characteristic of hypertrophic scar in rabbits. The conversion of normal scarring to hypertrophic scarring in rabbits occurs at 14~ 21 days after operation. Both development and regression of hypertrophic scar in rabbit are quicker than that in human.
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瘢痕组织中肥大细胞的光电镜观察
肥大细胞是机体组织中一种重要的细胞成分,主要功能为合成、贮存和释放肝素、组织胺等活性物质,在皮肤组织中存在较多,故在创伤愈合与瘢痕形成中有重要作用.笔者从组织形态学及超微结构观察瘢痕组织中肥大细胞的变化,探讨瘢痕的形成与发展,为瘢痕的防治提供依据.
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"瘢痕贴"防治创面增生性瘢痕的临床研究
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局部5-氨基酮戊酸光动力疗法治疗增生性瘢痕的进展
增生性瘢痕是病理性瘢痕的一种,为皮肤创伤愈合过程中成纤维细胞过量增殖和细胞外基质的过度沉积(主要为胶原纤维)[1],且增生性瘢痕增生期伴有过度和延长的血管增生.目前的治疗方法如:激素注射、放射治疗和冷冻治疗等疗效均不理想[2],且这些方法会诱导细胞坏死或形成新的损伤,从而诱导新一轮修复增殖效应,甚至愈演愈烈,所以,瘢痕形成的过程并未阻止,而仅仅是被推迟,且愈演愈烈.因此,寻找一种有效防止早期瘢痕增生、无创、简便且依从性好的治疗方法一直是瘢痕研究领域的热点.PDT为治疗肿瘤性疾病的新型治疗手段[3].1999年,美国FDA批准20% ALA (Levulan)局部溶液介导PDT可用于治疗光化性角化病[4].
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增生性瘢痕治疗靶点的回顾性分析
瘢痕是创伤愈合留下的痕迹,是高等哺乳动物组织修复过程中特有的愈合形式,增生性瘢痕(hypertrophic scar,HS)是皮肤组织损伤后过度修复和异常增生的结果,主要继发于累及真皮层的损伤,如:烧伤、外伤、外科手术等,其实质是成纤维细胞(fibroblast,FB)的过度分裂增殖并合成大量排列紊乱的胶原纤维以及细胞外基质(EMC)的过度沉积.
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增生性瘢痕的发病机理和预防进展
增生性瘢痕一直以来都是存在于全世界范围内的难题,在不同种族中其发病率有所差异,以往的报道提到白种人群中的增生性瘢痕发病率在5%~37%[1].增生性瘢痕在临床上表现为不超过伤口范围的高出皮肤表面的瘢痕组织,早期充血明显,可伴有疼痛或瘙痒,位于关节部位则可能会引起活动功能障碍[2].增生性瘢痕产生的不雅外观和不适症状让患者身心都承受着巨大的压力,预防瘢痕不仅是医学问题,也是一个社会问题,其防治对临床工作者来说更是一个挑战.本文就增生性瘢痕的预防进展综述如下.
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