AIM To evaluate the possibility of using cultured human hepatocytes as a bridge between bioartificial liver and liver transplantation.METHODS In this experiment, the efficacy of extracorporeal bioartificial liver support system (EBLSS) consisting of spheriodal human liver cells and cultured hepatocytes supernatant was assessed in vivo using galactosamine induced rabbit model of fulminant hepatic failure.RiESULTS There was no difference of survival between the two groups of rabbits, but in the supported rabbits serum alanine aminotransferase, total bilirubin and creatinine were significantly lower and hepatocyte necrosis was markedly milder than those in control animals. In addition, a good viability of human liver cells was noted after the experiment.CONCLUSION EBLSS plays a biologic role in maintaining and compensating the function of the liver.

Objective　To evaluate the efficacy and the mechanism of application of selective head cooling on neuronal morphological damage during postischemic reperfusion in a rabbit model.Methods　168 New Zealand rabbits were randomized into three groups. Group Ⅰ ［n=24, (38±0.5)℃, non-ischemic control］; Group Ⅱ ［n=72, (38±0.5)℃, normothermic reperfusion］; Group Ⅲ ［n=72, (28±0.5)℃, selective head cooling, initiated at the beginning of reperfusion). Animals in three subgroups (n=24, each) of Group Ⅱ and Group Ⅲ had reperfused lasting for 30, 180 and 360 min respectively. Using computerized image analysis technique on morphological changes of nucleus, the degree of neuronal damage in 12 regions were differentiated into type A (normal), type B (mild damaged), type C (severely damaged) and type D (necrotic). Fourteen biochemical parameters in brain tissues were measured.Results　As compared with Group Ⅰ, the counts of type A neuron decreased progressively, and those of type B, C and D increased significantly in Group Ⅱ during reperfusion (P<0.01). In Group Ⅱ, vasoactive intestinal peptide, b-endorphine, prostacyclin, T3 and Na+, K+-ATPase were correlated with the changes of type A; b-endorphine and thromboxane with type B; glucose and vasopressin with type C; Na+, K+-ATPase, glutamic acid, T3 and vasoactive intestinal peptide with type D (P<0.05). As compared with Group Ⅱ, the counts of type A increased, and those of type C and D significantly decreased in Group Ⅲ (P<0.01). In Group Ⅲ, Ca2+, Mg2+-ATPase were correlated with the changes of type A, C and D (P<0.01). Conclusion　Selective head cooling for sex hours during postischemic reperfusion does improve neuronal morphological outcomes in terms of morphological changes.

长期电刺激引起的机体紊乱

Objective To create an experimental model of IS intra vitam as well as post mortem assessment of the effect of LESS on the organism.Method The experiment was conducted on 10 rabbit males in developing stage (age 3.5 months). The animals were divided into two groups (n = 5): I in which long timed lateral electric surface stimulation (LESS lt) was applied for 9 h daily during 3 months, II control group, in which rabbits were not stimulated. Growing animals were used so that the investigations would correspond to clinical practice in which adolescents were treated for scoliosis. LESS was performed with SCOL 2, a Polish stimulator, which was placed to the right of the spine, at Th3 Th8 height, 2～ 3 cm below pectoral vertebrae. After 3 months the rabbits were sacrificed,and detailed macroscopic examination was performed.Results LESS statistically significant disturbs growth rate in terms of body mass gains. Individual body mass in this group amounted to 2100 g at the beginning of the experiment, and to 2320 g in the end. Post mortem investigation of LESS lt animals revealed congestion of liver and kidneys, and considerable enlargement of suprarenes.The observed disorders of examined rabbit behaviour in LESS lt group, as well as lesion of suprarenes, suggest stress reaction related to therapy based on this kind of LESS.Conclusions Long timed LESS induced statistically significant disorders of body mass gains in growing rabbits. Long timed LESS was clearly a stressor for the animals.

实验性短期电刺激引起的机体紊乱

Objective To determine the effect of LESS-st on the organism of developing rabbits,and provide reference for the treatment of idiopathic scoliosis.Method Investigations were carried out on 10 rabbits, males, 3,5 months, New Zealand race, divided into two equal groups (n = 5): I -in which short-timed lateral electric surface stimulation (LESS-st) was applied for 2 h daily during 3 months, II -control group, where animals were not stimulated. LESS was performed with SCOL-2, a Polish stimulator, which was placed to the right of the rabbit spine, according to the method modified by Kowalski. After 3 months the animals were sacrificed, and macroscopic examination was performed. Statistical analysis based on Student's t-test was carried out into account body mass and results of the macroscopic evaluation.Result Post mortem examination of the internal organs showed no pathologic lesions in the animals both group. Only mean mass of suprarenes in electrostimulated rabbits was 42.7 % bigger than in control ones.The obtained results suggest that LESS-st did not cause statistically significant disturbances of rabbit growth during a 3-month experiment. Small variations were observed only in the end phase of the investigation.Conclusion (1) Short-timed electrostimulation (2h/day) did not cause statistically significant disturbances of rabbit growth during a 3-month experiment.(2) Short-timed LESS resulted in some behavioural disturbances in rabbits.

兔耳增生性瘢痕的形态学观察与羟脯氨酸含量变化

Objective To establish animal model for hypertreophic scar and study the characteristics of its morphology and collagen metabolism.Methods A total of 64 wounds(diameter of 6 mm each ) with total skin loss were made on the ventral side of rabbit ear using a trephine. Morphology and collagen metabolism of scar wound were studied at 14,21,35,70 and 98 days after operation,respectively.Results There were 76% elevated scars developed (45/59 wounds) on the ventral side of rabbit ear on 21st and 35 days.The number of fibroblast decreased,but irregular-arranged fibers still presented in the elevated scars at 70 and 98 days after operation. Hydroxyproline content in elevated scars at 21 days was higher than that in normal skin(P< 0.05),and at 35 days was 3 times as that in normal skin and at 98 days was also markedly higher than that in normal skin(P< 0.05).Conclusion Excessive deposition of collagen is a characteristic of hypertrophic scar in rabbits. The conversion of normal scarring to hypertrophic scarring in rabbits occurs at 14～ 21 days after operation. Both development and regression of hypertrophic scar in rabbit are quicker than that in human.

淫羊藿苷后处理对心肌缺血再灌注损伤的保护作用研究

目的：观察淫羊藿苷（ icariin，ICA）后处理对心肌缺血／再灌注损伤的保护作用及其可能机制。方法：将24只家兔分为3组：假手术组、I／R组以及ICA后处理组。采用家兔在体模型，家兔麻醉后，开胸短暂结扎冠状动脉左前降支模拟缺血，缺血30min后松开结扎线进行再灌注，再灌注前10min耳缘静脉注射给药。灌注过程中检测左心室内压等心功能指标，再灌注180min后采血检测血清中SOD、MDA；随后处死动物，心脏染色测定心肌梗死面积，检测心肌组织中Bcl－2、Bax蛋白水平。结果：与假手术组比较，I／R组中LVSP、±dp／dtmax下降，LVEDP升高，HR降低；血清中SOD活性降低，MDA含量增高；Bcl－2、Bax蛋白表达量增高（P＜0．01，P＜0．05）。与I／R组比较，ICA后处理组中LVSP、±dp／dtmax升高，LVEDP下降；心梗面积减少；血清中SOD活性增高，MDA含量降低；Bcl－2蛋白表达量增高，Bax蛋白表达量降低，（P＜0．05）。结论：ICA后处理对MIRI造成的心脏损伤具有保护作用，能明显改善心功能各项指标，缩小心梗面积，其保护机制与减轻心肌氧化应激反应、抑制心肌细胞凋亡有关。