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淋巴瘤侵犯骨髓患者初次血常规综合指标分析
恶性淋巴瘤晚期易侵犯骨髓或外周血,即恶性淋巴瘤的白血病期,该期疗效差、预后不良,严重影响患者健康[1,2].因此,本文研究收集2010-2012年我院淋巴瘤侵犯骨髓(lymphoma bone marrow involvement,LBMI)患者的初次血常规结果,分析白细胞(white blood cell,WBC)、血红蛋白(hemoglobin,Hb)、血小板(platelet,PLT)及淋巴细胞百分比(L%)的特点,以探讨综合分析上述指标对早发现、早诊断、早治疗LBMI患者的临床意义.
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一例严重冷凝集样本的血常规检验解决方案
冷凝集一般源于体内冷凝集素过高而导致血液在体外、低温条件下产生的红细胞(red blood cell,RBC)自凝,从而影响血液检查,特别是导致血常规检查以及血型鉴定出现错误结果.冷凝集素是一种能在低于37℃条件下凝集细菌、RBC等颗粒抗原的抗体,引起凝集反应温度一般小于30℃,其高滴度在4℃时出现,温度上升到37℃时凝集现象可消失[1].近日在常规检验工作中发现一例严重的冷凝集样本,我们采取了多种检验方法进行处理,并终给出正确血常规检验结果.现报告如下.
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成人EB病毒感染相关性噬血综合征一例报告
1 病例资料患者,女,26岁,主诉"发热、皮疹、关节痛3个月"于2007年8月8日入我院急诊重症监护室(emergency intensive care unit,EICU).患者2007年5月5日始咽部疼痛,在家中自服阿莫西林,5月8日双下肢出现皮疹,不高于皮肤,红色有痒感,伴双手关节痛,皮疹逐渐扩散至全身,5月12日出现发热,体温(Temperature,T)38℃,就诊于天津市某医院,查血常规白细胞计数(white blood cell,WBC)11.3×109/L,中性粒细胞百分比(Neutrocyte,NE%)86.6%,予以地塞米松及抗炎药物(具体不详)治疗一周.患者体温仍高达40℃,并伴有寒战.
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He-Ne激光并回输疗法治疗冠心病33例
He-Ne激光血管内照射(laser intravascular irradiation,ILIB)及紫外线氧自血回输疗法(ultraviolet blood irradiation and oxygenation, UBIO)是国内近十几年发展起来的新方法,本文对 33例冠心病患者行 ILIB及 UBIO隔日交替治疗.治疗前后检测血清中丙二醛(malonyl dialdehyde, MDA)及红细胞超氧化物歧化酶 (red blood cell super-oxide dismutase, RBC-SOD) ,探讨 ILIB及 UBIO隔日交替治疗对氧自由基相关成份的影响.
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红细胞分布宽度与系统性红斑狼疮病情活动的相关性研究
红细胞分布宽度( red blood cell distribution width,RDW)为评估红细胞体积大小异质性的常规参数。过去研究发现, RDW是鉴别诊断地中海贫血、巨幼红细胞性贫血和缺铁性贫血的重要临床指标[1]。新研究发现,RDW可用于心血管疾病的预后判定,RDW升高可代表心血管疾病患者体内的炎症状态,但是其机制尚不清楚[2-3]。戴天医等[4]研究发现,在冠状动脉综合征患者中,RDW与CRP、ESR等炎症因子呈正相关。
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RDW评估非瓣膜性房颤患者缺血性脑卒中的临床研究
目的:本研究旨在探讨红细胞分布宽度(red blood cell distribution width,RDW)与非瓣膜性心房颤动患者缺血性脑卒中发生之间的关系,并分析其潜在作用机制。
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Objective:In order to explore the radioprotective effects of the expression of hematopoietic growth factors regulated by radio-inducible promoter on radiation injury. Methods:The human FL (Flt3 ligand) cDNA and EGFP (enhanced green fluorescent protein) cDNA were linked together with IRES and then inserted into the eukaryotic expression vector pCI-Egr, which was constructed by substituting CMV promoter in pCIneo with the Egr-1 promoter (Egr-EF). The vector was transferred into human bone marrow stromal cell line HFCL by lipofectin. The transduced cell clones (HFCL/EF) had been selected by the addition of G418. The cells were exposed to γ-radiation by 60 Co source for 0.5-20Gy. The expressions of transduced cells were detected with FACS, Northern blot ELISA and CFU assay. The HFCL/EF and CD34+ cells from human umbilical cord blood were one after the other transplanted i.v. into sublethally irradiated severe combined immunodeficient (SCID) mice. The white blood cell amount in peripheral blood and human cell engrafted in recipent mice were detected by flow cytometry and CFU-GM etc. Results:The activity of EGFP in transduced cells increased by 3.1 fold as compared to non-transduced cells at 18h after exposure to 2.5Gy. The amounts of secreted FL in serum-free supernatants of Egr-EF increased by 605.46±107.21pg/ml, which were significantly higher than the control group (214.45±35.61pg/ml). The effects of FL in HFCL/EF cultural supernatants on expansion of CD34+ cells derived from cord blood in the presence of SCF, IL-6 and IL-3 were also studied. The results showed that at day 10 of culture the number of CD34+ cells increased by 173. 09±11.58×103/ml, which was significantly higher than that of non-radiation group(68. 04± 13. 73 × 103/ml). It showed that radiation can enhance the ability of the supernatants containing FL of HFCL/EF to expand early hematopoietic progenitor cells and protect hematopoietic cells from radiation-injury effects. The HFCL/EF and CD34+cells from human umbilical cord blood were one after the other transplanted i. v. into sublethally irradiated severe combined immunodeficient (SCID) mice. In contrast to two control groups (HFCL and HFCL/F), HFCL/EF (the Egr-1 regulatory element-drived expression of FL gene therapy) resulted in a proportionally obvious increase in the number of the white blood cell at early stage after radiation, while no significant differences were found for CD45+ 、CD34+ cells in bone marrow cells. In contrast to two control groups (HFCL and HFCL/F), HFCL/EF (the Egr1 regulatory element-drived expression of FL gene therapy) resulted in a proportionally obvious increase in the number of the white blood cell at early stage after radiation, without significant differences being found for CD45+、CD34+ 、CFU-GM and marrow nucleared cells in bone marrow cells. Conclusions:The results suggested both in vivo and in vitro use of the gene therapy of FL gene regulated by Egr-1 promoter could protect hematopoiesis from irradiation-induced damage.
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Clinical Significance of Detection of Minimal Residual Disease by Flow Cytomelry
Children with acute lymphoblastic leukemia have an excellent prognosis,but many factors including age and initial white blood cell count,and genetic abnormalities in the blasts can affect outcome.
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细菌性食物中毒性肠炎40例白细胞变化及临床意义
本文作者对40例夏季急性细菌性食物中毒性肠炎病人进行了白细胞计数,并作血涂片进行白细胞分析,观察白细胞形态变化.
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应用Cs-3000PLUS血细胞分离机采集外周血干细胞病人的护理
自体外周血干细胞移植(APBSCT)因具有造血和免疫功能重建快、肿瘤细胞污染少等优点,成为治疗多种恶性肿瘤并使病人长期生存的有效治疗方法.
关键词: 血细胞分离机 自体外周血干细胞移植 病人 护理 blood cell stem cells 免疫功能重建 肿瘤细胞 治疗方法 恶性肿瘤 长期生存 造血 污染 -
婴幼儿缺铁性贫血与红细胞体积分布宽度参数的实验室探讨
红细胞体积分布宽度(red blood cell volume distribution width,RDW)是定量反映红细胞体积异质性的参数,客观地反映了红细胞的大小的程度.我们对诊断为缺铁性贫血的100例婴幼儿进行了RDW与婴幼儿缺铁性贫血之间的关系及临床分析,现报告如下.
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红细胞体积分布宽度与急性心肌梗死患者冠脉病变程度的相关性分析
急性心肌梗死起病急,病死率高,严重威胁着人类的生命和健康,冠状动脉造影作为冠心病诊断的“金标准”,可以更加准确的评估冠脉病变程度。红细胞分布宽度(red blood cell volume distribution width,RDW)是反映外周血红细胞体积异质性的参数,由反映红细胞体积大小的变异系数表示,常用于贫血的诊断和分类,尤其是缺铁性贫血的鉴别诊断。然而,近年来发现 RDW 增高与急性心肌梗死、心力衰竭的发生密切相关[1,2]。
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Cellular Engineering in Cell Biology
The behavior of leukocyte-surface interaction is studied in lught of its important robe in the infalammatory process and in the development of ovtificial organs associated with prolonged blood contact. The process of cell-surface interaction revolves around a complex balance of forces arising from hemodynamic shearing effect and the strength of adhesive bonds between cell and substrate.This balance depends strongly on cell deformability and the expression of cell adhesion molecules (CAMs).In the course of these studies,a novel side-view flow chamber system has been developed to examine leukocyte(white blood cell)deformation and adhesion to various surfaces (cultured vascular endothelium,reconstituted purified CAMs,or biomaterials )under flow conditions that simulate the in vivo envronment.
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流行性出血热患者血液流变学改变的初步观察
本文报告流行性出血热(EHF)患者105例血液流变学及甲皱微循环观察结果,表明EHF在病程早期血液浓稠性增高,全病程血液粘滞性增加,并有血细胞聚集性增强,晚期血液凝固性有所增加.提示血液粘度的异常可能是EHF微循环障碍的重要因素之一.动态观察血液流变学及甲皱微循环似可作为判断EHF的病情和预后指标之一,并有助于指导治疗.
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482例急性感染者APRP与白细胞数在检测诊断中的价值
机体在遭受严重疾病或创伤等造成炎症和组织坏死时,特别是病程在3周以内的即急性感染,血清中急性时相反应蛋白(acute phase reaction protein,APRP)可在短期内升高,影响疾病的演化和转归.这些指标较传统的炎症指标如发热、白细胞升高及分类异常、血沉加快更能早期反映炎症和感染的状态,并且与白细胞(white blood cell,WBC)数的变化密切相关.
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血细胞分析仪的溯源及国际参考方法
为加强对血细胞分析仪的溯源及国际参考方法了解,作者主要对血细胞计数分析(红细胞计数、白细胞计数、血小板计数,血红蛋白以及红细胞压积)的国际参考方法建立的过程和及其概要进行了阐述,并对国际参考方法与各实验室目前方法学的溯源性进行了论述.
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外周血白细胞计数在创伤时的变化
外周血白细胞(white blood cell,WBC)计数和中性粒细胞(polymorphonuclear neutrophil leukocytes,PMN)比值升高通常认为是机体对创伤、出血、炎症、感染等状态的一种反应,特别是在严重创伤这一病理过程中起一个重要角色.通过94例严重创伤患者外周血白细胞(WBC)计数和中性粒细胞(PMN)比值观察,探讨其在创伤时的变化规律.
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急性化脓性甲状腺炎四例报告
急性化脓性甲状腺炎并不多见,我院1978~1983年共收治4例.其病因不明,一般是由于感染引起.诊断多依据临床表现、实验室检查等.一旦确诊,立即切开减压,重症患儿常规行气管切开.
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前降钙素对小儿急性阑尾炎的诊断价值
急性阑尾炎(acute appendicitis)是小儿腹部外科常见的急腹症之一,特点是病史及临床表现不典型、病情进展快、易穿孔、易误诊.血清前降钙素(procalcitonin,PCT)是一个新型的炎症指标,但PCT对于小儿急性阑尾炎的诊断还未见系统报道.本文主要研究PCT在小儿急性阑尾炎时的诊断价值,并与传统的炎症指标白细胞(white blood cell,WBC)计数和C反应蛋白(C reactive protein,CRP)进行比较.
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糖尿病大鼠红细胞在脑微血管的粘附
红细胞(red blood cell, RBC)是血液中极其重要的细胞成分,糖尿病时红细胞可以与血管内皮细胞(endothelial cell, EC)粘附,RBC对EC的粘附增强与糖尿病血管并发症严重程度有关.