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AIM To study the agglutination pattern of Helicobacter pylori coccoid and spiral forms.METHODS Assays of agglutination and agglutination inhibition were applied using fifteen commercial lectins. RESULTS Strong agglutination was observed with mannose-specific Concanavalin A (Con A ),fucose-specific Tetragonolobus purpureas ( Lotus A ) and N-acetyl glucosamine-specific Triticum vulgaris (WGA) lectins. Mannose and fucose specific lectins were reactive with all strains of H. pylori coccoids as compared to the spirals. Specific carbohydrates, glycoproteins and mucin were shown to inhibit H. pylori lectin-agglutination reactions. Pre-treatment of the bacterial cells with formalin and sulphuric acid did not alter the agglutination patterns with lectins. However, sodium periodate treatment of bacterial cells were shown to inhibit agglutination reaction with Con A, Lotus A and WGA lectins. On the contrary, enzymatic treatment of coccoids and spirals did not show marked inhibition of H. pylori-lectin agglutination. Interestingly, heating of H.pylori cells at 60℃ for 1 hour was shown to augment the agglutination with all of the lectins tested. CONCLUSION The considerable differences in lectin agglutination patterns seen among the two differentiated forms of H. pylori might be attributable to the structural changes during theevents of morphological transformation,resulting in exposing or masking some of the sugar residues on the cell surface. Possibility of various sugar residues on the cell wall of the coccoids may allow them to bind to different carbohydrate receptors on gastric mucus and epithelial cells. The coccoids with adherence characteristics like the spirals could aid in the pathogenic process of Helicobacter infection.This may probably lead to different clinical outcome of H. pylori associated gastroduodenal disease.
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冠心病患者幽门螺杆菌感染调查
目前虽有多种方法可诊断幽门螺杆菌(Helicobacter pylori,Hp)的感染,但其结果易受试剂、标本、环境、用药情况、主观经验等因素的影响,而且样本采集常有侵入性和创伤性,给患者带来一定的痛苦和心理负担,限制了在临床的普及.
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努力提高幽门螺杆菌的根除率
随着幽门螺杆菌(Helicobacter pylori,Hp)感染在胃十二指肠疾病发病中的作用得到认识,20多年来人们一直在寻求高效、安全、价廉、服用方便的根除Hp方案,但迄今尚无一种方案能同时满足上述要求.20世纪90年代中期提出的质子泵抑制剂(PPI)加克拉霉素再加阿莫西林或甲硝唑的,以"PPI为基础、含克拉霉素的三联疗法"(标准三联疗法),由于根除率高、不良反应少而被推荐为根除Hp的一线方案.
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第五次全国幽门螺杆菌感染处理共识报告
The fifth national consensus conference on the management of Helicobacter pylori (H.pylori) infection organized by Chinese Medical Association,Chinese Society of Gastroenterology,H.pylori and Peptic Ulcer Study Group was held at Hangzhou,Zhejiang Province on December 15-16,2016.More than 80 members of the study group and experts in the field of H.pylori research and gastroenterology attended the meeting.Consensus preparatory group was established to draft the related statements.The quality of evidence and strength of recommendation were evaluated by GRADE system.The Delphi method using an anonymous electronic system was adopted to develop the consensus of relevant statements.Expert advices about the established statements were firstly consulted through the emails.After two rounds of consultation,the preliminary statements of consensus were discussed and modified in the conference item by item.A total of 21 core members voted for the final version,which contained a total of 48 statements and was divided into 6 parts,including indications for H.pylori eradication,diagnosis,treatment,H.pylori and gastric cancer,H.pylori infection in special populations,H.pylori and gastrointestinal microbiota.
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This paper reviews the recentHelicobacter infection associated with chronic liver disease. The bacteriology, prevalence, pathogenesis and diagnosis were reviewed. Future work should be conducted on the pathogenesis and treatment of this disease.
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幽门螺杆菌实验室诊断
精确的检查方法是诊断幽门螺杆菌(Helicobacter pylori,Hp)感染和证实疗效的关键.目前常用的Hp诊断方法可分为尿素酶依赖性试验、免疫学试验、Hp菌形态学观察以及细菌DNA检测4大类.从方式上,则可分为侵入性和非侵入性两大类.每一种诊断方法各有优缺点,适于不同的临床情况.
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幽门螺杆菌研究现状
自1983年Warren和Marshall[1]首次从胃黏膜中分离出幽门螺杆菌(Helicobacter pylori,Hp)以来,确立了Hp是慢性胃病的重要病因.进一步发现Hp不仅与系统疾病,如心血管、呼吸、血液、免疫、内分泌代谢、脑血管等疾病有关,而且也是某些皮肤、口腔、眼等疾病的病因或发病因素,因此,近20年Hp一直是研究的热点,而且取得了很大进展.
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Hp与胃癌的基础与临床研究进展
胃癌是消化道常见的恶性肿瘤,具有较高的死亡率.目前认为胃癌的发生与多种因素相关.自从人们发现幽门螺杆菌(Helicobacter Pylori,Hp)以来,该菌与胃癌的关系已经受到广泛关注.大量基础与临床的研究表明Hp是胃癌发生过程中的一个重要危险因素,起到启动因子作用.世界卫生组织已将Hp列为Ⅰ类致癌源.近年来有关Hp与胃癌关系的研究主要集中在动物模型的建立、人群干预试验、Hp致癌机制等.
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上海地区企事业单位人员幽门螺旋杆菌感染率调查
幽门螺旋杆菌(hehcobacter pylori,HP)感染是B型慢性胃炎和消化性溃疡的重要致病因素[1-2],1992年Mitchell等[3]对我国广东省1 727名城市人口调查的阳性率78%.为了解上海地区企事业单位人员HP感染发生率,我们对来我院体检并接受13C尿素呼气试验(13C-UBT)的4 642例健康体检者进行了调查分析.
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胃食管反流病与幽门螺杆菌感染
幽门螺杆菌(helicobacter hylori,Hp)是慢性胃炎的主要原因,在消化性溃疡的发生中起重要作用,根除Hp可促进溃疡愈合,明显减少溃疡病的复发.
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胃癌与幽门螺杆菌感染相互关系的研究进展
胃癌是一种常见的恶性肿瘤,其发生是多因素的[1,2].自Warren和Marshal[3]成功分离到幽门螺杆菌(Helicobacter pylori,Hp)后,众多研究揭示Hp感染作为环境因素,可能是胃癌发生的重要原因之一[4].
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四联疗法治疗幽门螺杆菌阳性十二指肠溃疡患者的成本——效果分析
幽门螺杆菌(H.pylori)是消化性溃疡的重要致病因素.因此根除H.pylori可提高溃疡的愈合率,并可明显减少溃疡的复发.我们采用RH2、果胶铋、阿莫西林和甲硝唑组成四联方案与PPI、克拉霉素、甲硝唑三联方案对十二指肠溃疡合并H.pylori感染的病例各60例进行临床疗效与费用效益比较,以寻找既安全、有效又价廉的治疗方法.
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胃食管反流病与幽门螺杆菌感染
幽门螺杆菌(Helicobacterpylori,Hp)是公认的慢性胃炎与消化性溃疡病的重要致病因子之一.近年来在溃疡病的治疗中,除研制出许多强力制酸药外,还着力寻求根治Hp的治疗方案.实践证明,抑酸剂加上敏感的抗菌素,明显提高了溃疡病的治疗效果,不仅可以促进溃疡的愈合,而且明显减少溃疡病的复发率.
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幽门螺杆菌基因多态性的地域差异对感染临床结局的影响
已知幽门螺杆菌(H.pylori)是引起人类慢性胃十二指肠疾病的主要致病菌.流行病学资料显示,全球半数以上人群存在H.pylori感染,但大多数感染者胃内并不发生病变,或表现为轻度慢性胃炎,仅小部分感染者发展为萎缩性胃炎、消化性溃疡、胃癌、胃黏膜相关淋巴组织(MALT)淋巴瘤等严重疾病.H.pylori感染临床结局的多样性与宿主遗传因素、环境因素、细菌本身的毒力因子及其间的相互作用有关.
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重视幽门螺杆菌耐药菌株的研究
幽门螺杆菌(H.pylori)的发现使慢性胃炎和消化性溃疡的病因学和治疗学发生了重大变革,H.pylori感染与慢性胃炎、消化性溃疡、胃癌以及胃黏膜相关淋巴组织(MALT)淋巴瘤密切相关已得到公认.因此,H.pylori感染的治疗已成为目前H.pylori研究领域的重点课题,也是临床医师为关注的问题之一.在H.pylori感染的治疗中,细菌对抗生素产生耐药是导致根除治疗失败的主要原因,因此H.pylori耐药菌株的研究已引起人们的普遍关注.
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高压氧综合治疗幽门螺杆菌阳性十二指肠溃疡60例临床观察
幽门螺杆菌(helicobacter pylori,HP)是消化性溃疡(peptic ulcer,PU)的主要致病因素之一,在十二指肠溃疡(duodenal ulcer,DU)中感染率更高[1].
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上消化道疾病患者幽门螺杆菌检出率及耐药情况
幽门螺杆菌(Helicobacter pylori,简称H.pylori或简写为HP)是慢性胃炎和消化性溃疡的重要致病因子,是胃粘膜相关性淋巴样组织(MALT)淋巴瘤的重要因素,是国际上公认的一类致癌因子[1].本文通过对宁波镇海地区上消化道疾病患者胃镜活检标本进行组织病理检查及HP培养、鉴定及药物敏感定量试验,旨在了解镇海地区上消化道疾病患者的HP感染率及HP耐药情况,为临床有效根除HP提供参考.
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利福霉素衍生物Rifabutin的临床药理研究进展
Rifabutin(RBT)是一种类似于利福平(RIF)的利福霉素衍生物.1992年被美国FDA批准用于治疗和预防发生在AIDS患者上的鸟分枝杆菌(MAC)感染,随后许多国家相继批准使用该药.但是后来RBT已不仅被用于预防治疗MAC,还用于治疗肺结核以及幽门螺杆菌(HP)感染.正是由于该药相对于其他利福霉素类药物的高度亲脂性和较弱的CYP450诱导作用使得它成功应用于临床多种疾病的治疗,尤其是和多种抗生素联合用药时,不仅部分解决了传统抗生素的细菌耐受性问题而且增加了临床联合用药的安全性.本文旨在阐述有关的Rifabutin的临床药理以及相关方面的研究进展,以便指导今后临床用药.
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三种幽门螺杆菌检测方法比较
幽门螺杆菌(Helicobacter Pylori.Hp)检查方法很多,用于临床诊断Hp的方法就包括形态学(涂片或染色)及尿素酶依赖性试验(快速尿素酶检测及13C或14C尿素呼气试验).快速尿素酶检测(RUT)及13C尿素呼气试验(13c-UBT)在基层的医院开展较多.RUT及13C-UBT虽然同属尿素酶依敕性试验,但由于其检测方法不同,其敏感性与特异性亦存在差异.本文旨在探讨胃粘膜组织切片Warthin-Starry银染色法(w-s银染)、快速尿素酶试验(RuT)及13C尿素呼气试验(13C-UBT)三种幽门螺杆菌(HP)检查方法的准确性、安全性及经济性.
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胃血色素指数的测定对幽门螺杆菌的诊断价值