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P物质在佐剂性关节炎发病中的作用
佐剂性关节炎(AA)是类风湿关节炎的动物模型。P物质(SP)是发现早的一种神经肽，广泛分布于外周及中枢神经系统中。有人报道P物质在外周及体外细胞培养条件下可增强免疫功能。本实验对佐剂性关节炎发病过程中P物质的可能调节作用进行了初步探讨。1 材料与方法 选用体重约200克左右的Wistar雄性大鼠，将0.1mL完全弗式佐剂(CFA，内含死结核杆菌1.0mg/mL)皮内注射于右后肢足垫内，观察AA发病情况。将雷公藤多甙片用1%的甲基纤维素配制成3g/L的混悬液，将发病动物于免疫后17d开始连续口服雷公藤多甙10mg/kg，每日1次，共10d，观察雷公藤多甙对AA的治疗效果；通过淋巴细胞转化实验反映细胞免疫功能。对中枢P物质进行放射免疫测定。实验数据以均值±标准差(x—±s)表达，用t检验作统计学处理。2 结果 以CFA皮内注射到大鼠右后肢足垫内，至免疫后第15d，按炎症情况分级，对侧即左后肢发生炎症者占总数的37.5%，严重者占22.5%。严重者(Ⅲ，Ⅳ级)左后踝周径为3.49±0.18cm，显著高于左后肢未发生炎症者2.40±0.02cm(P＜0.001)。左后肢炎症自CFA免疫后第28d开始消褪，自45d左右痊愈。Ⅰ-Ⅱ级炎症关节可完全恢复正常，Ⅲ-Ⅳ级炎症关节大多仍僵直。结果说明佐剂性关节炎是一种自限性疾病，可以由注射CFA诱导出来。注射侧即右后肢炎症持续三个月仍存在。CFA注射组的体重增加20.2±2.3g，明显低于对照组不完全弗氏佐剂(IFA)注射组55.1±5.7 g (P＜0.01)。 在此期间给雷公藤多甙组体重增加23.1±3.8 g，明显高于对照组6.6±2.2g(P＜0.01)。 免疫对淋巴细胞转化的影响：于免疫后当天，第6、25、51d进行淋巴细胞转化实验，测定counts/min值。结果表明，CFA免疫可以增加机体免疫功能，且对免疫功能影影响较持久(见表1)
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Relationship Between and Laser Acupuncture Analgesia and the Function of Mast Cells in Adjuvant Arthritis Rats Treated by Acupuncture
Objective:To observe the analgesia effect and of low-level combined- and single-laser irradiation on Zusanli (ST 36) in rats and the relationship between mast cell degranulation and laser acupuncture analgesia.Methods: A total of 66 Sprague Dawley (SD) rats were randomly assigned into normal control,model control,sham irradiation,10.6 μm laser (220 mW,40 Hz) 650nm laser(36mW,continuous),combined laser (10.6 μm+ 650 nm) groups.Arthritis mode 1 was established by injection of Complete Freund's Adjuvant (0.05 mL) into the left ankle joint.The paw withdrawal latency (PWL) was detected with a radiant heat algesimeter.Zusanli (ST 36) was irradiated with sham,single or combined laser for 30 min.After sacrifice under anesthesia (1% embutal),tissues of Zusanli (ST 36) area were sampled,sliced (5 μm) and stained with Toluidine Blue for skin for observing the mast cell degranulation.Results:Compared with model control and sham group,the pain threshold increased significantly in 650nm and combined laser groups (P＜0.01),while remained no significant difference in 10.6 μm group.Compared with model and sham group,the degranulation ratios of mast cells in 650nm and combined laser group were significantly higher (P＜0.001),while remained no significant difference in 10.6 μm group.The linear correlation coefficient between degranulation ratios of mast cells and PWL after laser acupuncture is 0.737 (P＜0.001).Conclusion:Both 650 nm and combined laser stimulation of Zusanli (ST 36) can effectively raise pain threshold,and enhance degranulation ratio of mast cells at the stimulated acupoint.The result also suggested a linear correlation between degranulation ratio of mast cell and analgesia effect.
