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儿童急性淋巴细胞白血病微小残留病技术方案
微小残留病(MRD)是急性淋巴细胞白血病(ALL)患儿经过治疗,并按目前所确定的疗效标准(骨髓中原始或幼稚肿瘤细胞在5%以下,患者的病情完全控制)达到临床完全缓解状态后,体内残留微量白血病细胞的状态.是ALL的重要的独立预后指标[1],已经证实患儿体内的MRD水平在ALL的疗效评价、预后判断和复发监测以及造血干细胞移植时净化移植物等方面均有十分重要的意义,多家国外先进治疗组采用MRD水平进行儿童ALL的危险度分层和预后评估,取得了良好的效果[2-8].目前国内外儿童白血病协作组已经将MRD的检测作为重要的危险因素评价指标之一,以诱导治疗早期、结束时MRD水平进行危险度分层,将调整治疗纳入到治疗方案中[9-10],在获得大的疗效同时减少化疗药物的毒性,对易复发的患儿予以强化治疗,提高患儿的生存率以及生活质量.
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Objective: To evaluate the significance of two-color interphase fluorescence in situ hybridization (FISH) using X and Y centromere probe in the engraftment estimation and minimal residual disease (MRD) monitoring after allogeneic stem cell transplantation (alloSCT). Methods: Samples from 12 cases patients in different periods after alloSCT were detected by interphase FISH. Results: All of the 12 patients were proved to obtain engraftment 22(35 days after alloSCT. While traditional karyotype showed as 100%XX or 100%XY invariably, FISH showed different percentages of donor original sex chromosome. Conclusion: Two-color interphase FISH is a more sensitive and simple test for engraftment evaluation and MRD monitoring post SCT, though, it can not entirely replace traditional karyotype analysis and gene detection by RT-PCR.
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浆细胞样树突细胞与儿童急性淋巴细胞白血病微量残留病的相关性研究
急性淋巴细胞白血病(ALL)是儿童常见的恶性肿瘤,随着化疗方法的改进,目前ALL患儿5年无病生存率可达70%~83%,但仍有20%~30%的患儿因微量残留病(MRD)的存在终复发[1].此时化疗的作用十分有限,造血干细胞移植因供者来源不足及费用高昂,很难普遍适用.所以,在ALL完全缓解后监测MRD并应用新的治疗手段,如免疫治疗清除MRD就成了必要选择.