腹腔镜和传统腹股沟疝修补术对小儿免疫功能的影响

腹腔镜下疝修补术是近年来小儿外科发展较快的一种手术方式,其特点是创伤小、住院时间短、机体恢复快[1,2].同时腹腔镜手术对患者免疫功能的影响也引人关注.有报道腹腔镜下小儿隐睾复位手术对机体免疫功能的损伤较开放手术小[3].但是腹腔镜对小儿疝修补术免疫功能的影响如何尚不清楚.本研究拟探讨腹腔镜下疝修补术和传统外科开放疝修补术对机体免疫功能的影响.

Chickens were infected with CAV at one-day-old and 8 days later, the infected and uninfected chickens were vaccinated with La Sota vaccine. At 7\, 14\, 28 days post vaccination, the number of T cells and IgG, IgM and IgA antibody producing cells in Harderian gland and cecal tonsil, the content of IgG, IgM and IgA in tear, trachea fluid, intestinal fluid and bile as well as the hemoagglutination inhibition (HI) titer in tear and bile were detected. The results showed that the number of T cells and IgG, IgM and IgA antibody producing cells in Harderian gland and cecal consil, the content of IgG, IgM and IgA in tear, trachea fluid, intestinal fluid and bile as well as the HI titer in tear and bile post ND vaccination of CAV infected chickens were decreased significantly than those of uninfected vaccinated chickens. These indicated that the immune response function was markedly weakened in local mucosa of digestive and respiratory tract post ND vaccination of CAV-infected chickens.

墨旱莲醋酸乙酯总提取物对免疫功能的影响

中药墨旱莲为菊科鳢肠属植物鳢肠(Eclipta prostrata)的干燥地上部分,具有滋肝补血、凉血止血之功能,用于阴虚血热、吐血、衄血、尿血、血痢、崩漏下血、外伤出血等症[1].近年研究表明墨旱莲有较强的免疫调节作用、止血、保肝、抗炎等多种药理作用,临床上用于多种疾病的治疗[2].为进一步探讨其免疫调节作用机理及寻找其有效成分,本研究从整体水平观察墨旱莲醋酸乙酯总提物(EAEEP)对机体非特异免疫和体液免疫的影响.

Objective:To investigate the effects of different anesthesia methods on perioperative immune function in patients with hepatic cirrhosis.Methods: Fifty cases of patients with hepatic cirrhosis who were included in our hospital from January 2011 to January 2012 received elective splenectomy devascularization under intubation and general anesthesia were selected and randomly divided into the treatment group and the control group, each group with 25 cases. The treatment group was given intravenous-inhalation combined anesthesia; the control group was given total intravenous anesthesia. Then immune function and recurrence rates of both groups were evaluated.Results: At T0 moment, CD3+, CD4+, CD8+, CD4+/CD8+ and NK cell numbers of both groups showed no statistical difference. Compared with those at T0, CD3+, CD4+, CD8+, CD4+/CD8+ and NK cell numbers of both groups at T1 moment significantly decreased; at T2 moment, CD3+, CD4+ and CD4+/CD8+ of treatment group were significantly higher than those of the control group. Overall study showed that the comparison between groups and time points as well as cross-comparison between two groups and time showed no significant difference. For the comparison between groups, at T0 moment, numbers of B lymphocytes showed no significant difference; the comparison of those at T1, T2 and T0 moment showed no significant difference, either. At T1, T2 and T0 moment, there were also no significant differences between groups. At T1 moment, INF-γ levels of both groups were lower than those at T0; there was restoration at T2 moment. At T1 moment, sIL-2R levels of both groups were lower than those at T0; there was slight restoration at T2 moment. For both groups, there were no significant differences at T1 and T2 moment. Recurrence rates and 5-year survival rates of two anesthesia methods showed no significant difference.Conclusion:Intravenous-inhalation combined anesthesia is helpful to restore cellular immune function of patients with hepatic cirrhosis and increase the safety of treatment; it is worthy of clinical application.