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RSM-DSA技术与临床应用体会
实时平滑蒙片法(Realtime smoothed Mask DSA,RSMDSA),是日本岛津公司的全球专利.在国内,此方法在临床巾的应用尚未见报道.我们将RSM DSA这一新型的时间减影方法应用于临床,特别是应用于下肢动脉造影巾,利用RSM-DSA减影法与一般DSA方式减影相比较,得到的优越性及其临床应用体会做出介绍,仪供参考.
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丹参、黄芪合用对脑缺血再灌注后脑组织Fos,Jun蛋白表达的影响
AIM: To investigate the expression of Fos and Jun proteins in rat brainsafter focal cerebral ischemia followed by reperfusion and effects of RSMand astragus. METHODS: 30 SD adult male rats were divided into 5groups at random . Group A: sham-operated group; Group B: model group;Group C: treated with RSM; Group D: treated with astragus; Group E:treated with RSM and astragus. The immunohistochemistry and medicalimage processing system(MIPS) were used to measure the numbers andmean grey levels of Fos and Jun protein positive cells in rat cerebralcortex of 5 groups. RESULTS: ( 1 ) In cerebral cortex of group B , C , D, E, the numbers of Fos and Jun positive cells were more than those ingroup A and mean grey levels of Fos and Jun positive cells were lower thanthose in group A(P ＜ 0.01); (2) In cerebral cortex of ischemic sidesin group C, D, E,the numbers of Fos and Jun positive cells were less thanthose in group B and mean grey levels of Fos and Jun positive cells werehigher than those in group B(P ＜ 0.01) ; (3) Group E had more sig-nificant effects than group C or group D ( P ＜ 0. 01 ). CONCLUSION:The expression of Fos protein and Jun protein in model group increasedsignificantly, compared with sham-operated group; RSM , astragus , RSMand astragus all could inhibit partly the expression of Fos protein and Junprotein after cerebral ischemia and reperfusion; Prescription of RSM andastragus had stronger inhibiting effects than RSM or astragus. It may be oneof mechanisms that ischemic stoke is treated by reinforcing Qi and acti-vating blood circulation therapy in TCM clinic.
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基于RSM法优化补骨脂中总呋喃香豆素的提取工艺
目的 采用响应面法(RSM)优化补骨脂中总呋喃香豆素的提取工艺.方法 按照Box-Benhnken设计原理,以液料比、提取温度和聚酰胺用量为主要影响因素,以补骨脂中总呋喃香豆素量为评价指标,优选佳提取工艺.结果 佳提取工艺为加18倍量的甲醇,80℃水浴提取2次(1.5h/次),滤液加2.1倍量的聚酰胺粉末搅拌均匀,挥干甲醇,上柱,30％乙醇洗脱,在该条件下总呋喃香豆素量的理论值为1.13％,验证值为1.16％,两者相差2.59％,验证值与理论值相吻合.结论 RSM法优化补骨脂中总呋喃香豆素的提取工艺简便易行,且预测性好,便于实际应用.
关键词： 补骨脂 总呋喃香豆素 RSM Box-Benhken中心组合
