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氦氖激光诱导培养瘢痕成纤维细胞凋亡的实验研究
Objective To explore He- Ne laser inducing apoptosis of cultured fibroblasts in hypertrophic scars(HS).Methods Cultured fibroblasts in HS were irradiated with He- Ne laser. Apoptosis was examined by TUNEL technique and cell necrosis was counted by trypan blue staining. Results There was no cell apoptosis after repeated irradiation with 10mW/cm2 and 50mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 7.20% and 12.73% after repeated irradiation with 100mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 14.74% and 16.27% after laser irradiation with 150mW/cm2 for 10 and 30 minutes.No necrotic cell was presented after repeated irradiation for 10 and 30 minutes at various power densities. Conclusion Repeated He- Ne irradiation of HS- derived fibroblast cultures at power densities of 100mW/cm2 and 150 mW/cm2 can induce cell apoptosis. Power density is more important than energy density so far as laser- inducing apoptosis of scar fibroblasts concerned.
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低强度He-Ne激光照射对冠心病患者氧自由基相关成分影响
低强度He-Ne激光血管内照射治疗(ILLLI)是近十几年发展起来的新方法.本文对32例冠心病患者行低强度He-Ne激光血管内照射治疗前后,检测血清中丙二醛(MDA)及红细胞超氧化物歧化酶(RBC-SOD),探讨低强度He-Ne激光血管内照射对氧自由基的相关成分影响.
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川芎嗪联合低能量He-Ne激光血管内照射治疗脑梗塞的疗效观察
目的:探讨川芎嗪联合低能量He-Ne激光血管内照射(ILIB)治疗脑梗塞的临床疗效.方法:用川芎嗪联合ILIB治疗32例经头颅CT证实的急性脑梗塞患者,其中轻型16例,中型13例,重型3例.结果:大多数患者神经功能缺损有很大的改善(P<0.01),日常生活能力有较大提高(P<0.01).其中痊愈9例,显著进步15例,进步7例,总有效率96.88%.结论:川芎嗪联合ILIB治疗急性脑梗塞疗效较好.
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激光血管内照射治疗85例脑血管病恢复期患者的观察与护理
低能量氦-氖(He-Ne)激光血管内照射疗法是近年来国内开展的一项新的理疗方法,对于脑血管病恢复期的康复治疗有较好的促进作用,可以改善血液流变学,增加病变部位的有效血循环血量,激活生物酶活性,促进组织新陈代谢,加速代谢物质及炎性物质的吸收,有利于缺血组织的康复和再生.