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Calcium alginate gel (CAG) has been shown to successfully model aneurysm embolization within a short period of time. However, gradually degrading CAG potentially results in aneurysm recanalization.In the present study, a regenerative embolic material was designed by seeding rat fibroblasts in a CAG. The study investigated the feasibility of constructing a 3-dimensional culture system. The fibroblasts grew well and firmly attached to the CAG. CAG was conducive for fibroblast growth, and resulted in a 3-dimensional culture system. Results show that CAG can be used theoretically as a vascular, regenerative, embolic material.
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氦氖激光诱导培养瘢痕成纤维细胞凋亡的实验研究
Objective To explore He- Ne laser inducing apoptosis of cultured fibroblasts in hypertrophic scars(HS).Methods Cultured fibroblasts in HS were irradiated with He- Ne laser. Apoptosis was examined by TUNEL technique and cell necrosis was counted by trypan blue staining. Results There was no cell apoptosis after repeated irradiation with 10mW/cm2 and 50mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 7.20% and 12.73% after repeated irradiation with 100mW/cm2 for 10 and 30 minutes. The percent of apoptosis was 14.74% and 16.27% after laser irradiation with 150mW/cm2 for 10 and 30 minutes.No necrotic cell was presented after repeated irradiation for 10 and 30 minutes at various power densities. Conclusion Repeated He- Ne irradiation of HS- derived fibroblast cultures at power densities of 100mW/cm2 and 150 mW/cm2 can induce cell apoptosis. Power density is more important than energy density so far as laser- inducing apoptosis of scar fibroblasts concerned.
