儿童生长激素与性激素复合刺激试验的护理

儿童生长激素释放激素(GnRH)和黄体生成索释放激素(LHRH)复合刺激试验是通过使用可使性激素和生长激素分泌增加的方法或药物,观察血液中生长激素和性激素的动态变化,从而了解下丘脑和垂体调节、合成与分泌生长激素和性激素的能力~([1]).主要适用于诊断生长激素缺乏症(GHD)所致的身材矮小和中枢性性早熟(CPP).对GHD和CPP的患儿进行GnRH和LHRH复合刺激试验,可对患儿的身材矮小和中枢性性早熟进行及早的病因诊断、早期的干预和治疗,从而改善患儿的身高和性器官的发育进程,有利于患儿的身体健康发育和身心健康.2007年1月至2009年9月我科对392例患儿进行了生长激素和性激素复合刺激试验.所有患儿均完成了试验,且试验安全可行,本文将护理经验进行总结,现报道如下.

Objective:The aim of our study was to measure and compare the serum hormone level of transplant group with blank control and castrated control groups after heterotopic autotransplantation of cryopreserved-thawed ovarian tissues into back muscles. Methods:A total of 40 SPF-SD female rats (5-6 week-old) were randomly divided into three groups:blank control group (group A), castration control group (group B) and transplant group (group C). Ovaries were removed by surgical procedure, then after cryopreservation and thawing procedures the ovarian tissues were implanted into the back muscles of mice in group C. After 4 weeks of ovarian tissues transplantation, al rats blood sampling were measured for E2, LH and FSH hormone levels by ELISA. Results:E2 level was significantly higher in group C and group A than group B [(38.98 ± 5.66) pg/mL, (8.14 ± 3.24) pg/mL;P<0.05) and [(36.30 ± 6.90) pg/mL, (8.14 ± 3.24) pg/mL;P<0.05)]. However, E2 level in group C and group A had no significant dif erence. FSH level in group B, group A and group C was (18.87 ± 2.54) nmol/L, (7.77 ± 0.87) nmol/L and (9.39 ± 2.12) nmol/L respectively. FSH level increased significantly in group B compared with group A, and the dif erence had statistical significance (P<0.05). FSH level was slightly increased in group C compared with group A, and the dif erence was not statistical y significant (P>0.05), but compared with group B, FSH level was significantly reduced and being statistical y significant (P<0.05). Conclusion:Autotransplantation of cryopreserved-thawed ovarian tissue into back muscles can sustain fol icular development and re-establish endogenous hormone production by restoring the factors such as angiogenesis and innervations at the graft site.