Objective. To study the redistribution of endothelin-1 (ET-1) receptors in two subcellular organelles , the sarcolemmal membrane and the light vesicle, of rat heart during the progression of sepsis. Methods. Sepsis was induced by cecal ligation and puncture (CLP). ET1 receptor was assayed by using [125I]-ET1 binding. Marker enzyme activities, protein yield, and dry-to-wet weight ratio of cardiac membranes were measured. Results. Septic rat heart exhibited two distinct phases: an initial hyperdynamic phase( 9h after CLP; early stage of sepsis) followed by a hypodynamic (18h after CLP, late stage of sepsis) phase. [125I]-ET1 binding study showed that during early stage of sepsis, the Bmax of ET1 receptors was increased by 30％ in sarcolemma but decreased by 19％ in light vesicles, while during late stage of sepsis, the Bmax was decreased by 24％ in sarcolemma but increased by 38％ in light vesicles.The total binding of sarcolemma and light vesicles was increased by 25％ during early stage of sepsis but decreased by 17％ during late stage of sepsis. Conclusions. These data indicated that ET1 receptors in the rat heart were externalized from light vesicles to sarcolemmal membranes during early hyperdynamic phase while internalized from surface membranes to intracellular compartment during late hypodynamic phase of sepsis.

婴幼儿急性坏死性软组织感染25例

急性感染是小儿常见病,发生在婴幼儿急性坏死性软组织感染,病程险恶,进展迅速,一旦延误治疗,往往造成死亡.我院自1974年1月至1985年1月共收治25例,现报告如下:一般资料本组男18例,女7例,年龄3个月到1岁半,其中5个月到1岁者11例,占40.4%.发病部位以腰,臀部为多,并向下腹部及大腿蔓延,本组18例,占79.2%.胸壁6例,颈背部1例.有局部注射史者8例,感染(水泡,疹子)轻微外伤者7例,其中1例为腹股沟疝复位后发病,其余10例病史不清.

1 IntroductionTwo new fiber-optic chemical sensor based on multiple fluorescence quenching is described. The reagent phases of the sensors are stable in organic solvent. The first reagent phase was constructed by covalent bonding pyrenebutyric acid (PBA) to the surface of glass (PBA-SiO2). It was identified by IR spectrum, fluorescence spectra and TGA analysis. And it can determine Rutin in ethanol.