摘要： SET is a multifunctional protein involved in regulating many biological processes of the cell cycle. It is also a regulator of steroidogenesis in the ovary. However, the expression of SET protein in testis, and its function, still remains ambiguous. In this study, we observed the expression of SET in the testes of mice at different developmental stages, and have discussed its potential function in regulating spermatogenesis and androgen production. Forty?eight male mice at different developmental stages(1week old as the infancy group; 4weeks old as the prepubertal group; 12weeks old as the adult group; over12months old as the ageing group) were used. Cellular location of SET protein in the testes was observed by immuno?histochemistry. Expression levels of Set mRNA and SET protein were analyzed by quantitative polymerase chain reaction and Western blotting. SET protein was expressed in spermatogonial cells and spermatocytes; the highest level was mainly in haploid and tetraploid cells of the prepubertal and adult groups, and Leydig cells of the adult and ageing groups. There was a low expression in Sertoli cells. Expression of Set mRNA in the prepubertal group was signiifcantly higher than that in the adult group(P<0.05), while expression of SET protein was at the highest level in the adult group(P<0.05).SET protein is mainly expressed in spermatogonial cells and spermatocytes, and poorly expressed in Sertoli cells, suggesting that it is involved in spermatogenesis. Expression of SET protein in Leydig cells suggests a possible role in steroidogenesis.
-
生活方式对亚洲男性性激素、性活动以及老龄化的调节作用
-
70W和120W2微米激光汽化切除良性前列腺增生的体外对比研究
-
中国人类精子库捐精现状及应用:7年多中心回顾性研究
-
西地那非联合多沙唑嗪缓释剂治疗勃起功能障碍合并良性前列腺增生相关下尿路症状患者疗效的开放对照多中心临床评估
-
体重指数及血脂水平与血清前列腺特异抗原的关系:一组中国50岁以下人群的研究
-
血清PSA水平与非癌变前列腺组织体积的比值是接受前列腺癌根治术患者局部晚期前列腺癌生化复发的新指标
-
人精子纤维鞘内CABYR蛋白与AKAP3及Ropporin蛋白结合
-
芳香化酶(CYP19)基因变体同精子浓度和活性的关系
-
治疗前列腺癌的化疗新策略:一种新的Eg5抑制剂S-(methoxytrityl)-L-cysteine
-
ERβ基因RsaI多态性可能降低精子受精力及胚胎的发育潜能
