Objective.To investigate the serum sE cadherin level in patients with endometriosis and the alterations of that level in healthy control during the menstrual cycle.Methods.Thirty two patients with endometriosis and 30 healthy women were tested for serum sE cadherin levels by enzyme linked immunosorbent assay.Results.The serum sE cadherin levels in healthy control did not vary throughout the menstrual cycle,which were lower than those in patients with endometriosis.Conclusions.E cadherin might be involved in endometrial shedding during menstruation in endometriosis patients.The serum sE cadherin assay might be helpful as a serum marker for the diagnosis and management of endometriosis.

Objective:To investigate the effects of interleukin-1beta (IL-1β) on expression of IL-8 in endometrial stromal cells (ESC) and evaluate the relationship between IL1 β and IL-8 ,and the significance of IL-1β in the development of endometriosis. Methods:The endometrial stromal cells obtained from patient with and without endometriosis cultured within 3 ～5 passage were exposed to various concentrations of IL-1β. The amount of IL-8 protein was assessed by ELISA. The expression of IL-8 mRNA was determined by RT-PCR. Results: 1. IL-8 protein was detected in culture supernatant of which the cells were not treated with IL-1β. The amount of IL-8 protein secretion increased obviously after stimulation with IL-1β at 1.0ng/ml for 4h and the peak of secretion was at 12h. 2. Expression of IL-8 mRNA was positive in unstimulated endometrial stromal cells. However, after stromal cells were incubated with IL-1β, the intensity of expression of IL-8 mRNA was obviously increased and demonstrated a dose-and timedependent manner. Increase of IL-8 mRNA was observed following stimulation with IL-1β for 4h ,and the peak at 12h. Conclusions:IL-1β induces endometrial stromal cell of endometriosis to express IL-8 not only at transcription level but also at post-transcription level. This up-regulation is dose-and time-dependent. IL-1β may play an important role in the onset of endometriosis.

Objective:To determine the role of Interleukine-8 in the pathogenesis of endometriosis(EM).Methods:36 patients with endometriosis were selected as study group.20 patients without endometriosis served as control group. IL-8 concentration in peritoneal fluid(PF) and serum of both groups were detected by ELISA.The correlation between IL-8 concentration and the severity of EM were performed.The differences of IL-8 mRNA expression in eutopic endometrium of patients between with and without endometriosis, and the differences among different endometriotic lesions were further studied by Northern Blot;Cultured endometrial stromal cells(CESC) were divided into groups. IL-8 and anti-IL-8 were used to treat these cells.Results:(1)The PF and serum from patients with EM contained significantly greater amounts of IL-8 than those in controls. A significant correlation between PF IL-8 content and the severity of disease was noted but there were no evidences of a relationship between concentrations of serum IL-8 and PF IL-8.(2) It was showed that the expression density of IL-8 mRNA of Eu were significantly higher than that of En. Among the three different endometriotic lesions, the highest IL-8 mRNA expression density was found in RPL(red peritoneal lesion), while the lowest IL-8mRNA expression density was detected in ULN(uterosacral ligament module).(3) There was a dose-dependent stimulatory effect of IL-8 on the survival of ESC. At 1ng/ml, anti-IL-8 significantly inhibited the survival of endometrial cells.Conclusion:IL-8 may be one of the essential factors for the pathogenesis of endometriosis.

Objective The ultrastructure of endometrium women suffering from endometriosis was compared with those from women of normal reproductive age.Materials & Methods Six specimens of eutopic endometrium were taken from women diagnosed with endometriosis. Three specimens were taken from women at normal reproductive-age. The specimens, all at early proliferative phase, were processed for ultrastructural study.Results Compared with the normal, the secretory cells of the epithelium of endometrium from women with endometriosis had more numerous and longer microvil li. In the cytoplasm, there were more secretory granules and mitochondria. The RER was more prominent and with dilated cisternae. Lipid droplets and electron dense particles were frequently seen. The cilia ciliated cells were increased and elongated remarkably. The basement membrane became markedly tortouas.Conclusion The endometrium from women with endometriosis showed a number of features which are abnormal. This might be the origin of endometriosis and a cause o f sterility.

Objective:To study the effect of preoperative compound rhizomacurcumaepowder treatment on serum markers and related signaling pathways function in endometrial tissue of patients with endometriosis.Methods:Endometriosis patients in our hospital were chosen for study and randomly divided into the treatment group (n=35) and the control group (n=35). The treatment group received preoperative compound rhizomacurcumaepowder treatment; the control group received preoperative conventional treatment. Then serum markers and function of related signaling pathways in endometrial tissues were compared.Results:After drug treatment, serum SCF, SDC-1, HLA-G, VEGF and Ang-2 contents of treatment group showed a significantly decreasing trend; mRNA contents and protein contents of Wnt, Frizzled, Dsh,β-catenin, cyclinD1, integrinα6β1, ERK1, ERK2, MMP9 and MMP27 in ectopic endometrial tissue of the treatment group all showed a significantly decreasing trend.Conclusion:Preoperative compound rhizomacurcumaepowder treatment is helpful to control the condition of endometriosis and inhibit the activation of cell cycle related signaling pathways and invasion related signaling pathways in ectopic endometrial tissue.

Objective:To explore molecular mechanism and biological function of miR-155 and its target genes on endometriosis.Methods: The expression of miR-155 in Ems patient and healthy control were assayed by RT-PCR. After miR-155 mimic and inhibitor were transfected into Ems endometrial cells for 48 h, the viability of cell was detected by MTT assay. Transwell migration and invasion assay were used to detect cell migration and invasion. The expression of cell apoptotic protein Bax and Bcl-2, matrix metalloproteinase (MMP 2) and MMP 9 were assayed by western blot.Results: The expression of miR-155 in Ems patient was more than that in the health control (P<0.01). After miR-155 mimic and inhibitor were transfected into Ems endometrial cells for 48 h, miR-155 over-expression could increase cell viability, and promoted cell migration and invasion, which was related to down-regulation of Bax along with up-regulation of Bcl-2, MMP 2 and MMP 9.Conclusion:These results suggested miR-155 lower expression inhibit endometrial cell proliferation and migration of the Ems.