补肾活血方对去势骨质疏松大鼠循环 Serotonin 表达的影响

目的：探讨补肾活血方对去势大鼠循环5-羟色胺（ Serotonin ）表达水平的影响。方法30只6月龄SD雌性大鼠去卵巢制作骨质疏松模型，另取10只行假手术，建立假手术组。3个月后，卵巢切除的大鼠随机分为三组，然后分别予补肾活血方（中药组），阿仑膦酸钠（西药组）和生理盐水（模型组）治疗，假手术组给予等量生理盐水。经过8周的治疗后处死大鼠，检测左侧股骨骨密度，ELISA检测血清Serotonin、核因子κB受体活化因子配体（ RANKL）和骨转换指标P1NP、CTX水平。结果模型组骨密度低于假手术组（ P＜0.001）；中药组和西药组全段股骨骨密度高于模型组（ P＜0.01和P＜0.001）。中药组和西药组血清P1NP低于模型组（P均＜0.001），而假手术组、中药组与西药组之间无统计学差异。中药组和西药组血清CTX低于模型组（P均＜0.001），高于假手术组（P均＜0.001）。中药组血清Serotonin水平低于西药组和模型组（P均＜0.001），假手术组与中药组之间无统计学差异。中药组和西药组血清RANKL低于模型组（ P均＜0.001），假手术组、中药组与西药组之间无统计学差异。结论补肾活血方可能是通过降低去势大鼠循环Serotonin表达水平，进而抑制RANKL表达水平，从而提高去势大鼠骨密度。

Objective Immediate physical exercise has been recommended for patients in the recovery phase to improve survival and quality of life ( QOL ) and reduce recurrence of disease .The new NCCN Guidelines for Survivorship also highlighted the role of exercise in post -cancer health , encouraging patients to perform light physical activity following treatment .The aim of our study is to effect of Tai Chi Chuan ( TCC) on serotonin and cortisol for monitoring stress and QOL in post-treatment breast cancer patients .Methods Totally 85 post-treatment breast cancer patients were enrolled in this study to observe the effects of practicing TCC on recovery , as well as stress and happiness which are indicators of QOL of in patients .Peripheral blood was drawn from study subjects to analyze the levels of serotonin , cortisol and high sensitive C-reactive protein ( HS-CRP) at baseline, and at 3, 6 and 12 months of TCC practice .Blood was drawn from healthy subjects only at baseline . A QOL questionnaire was administered to study subjects at three time points throughout the study , and once for healthy controls.The data were processed by analysis of variance of repeated measurement .Results At 3, 6 and 12 months time points following regular TCC exercise , WBC, RBC, hemoglobin in blood samples showed a statistically significant difference ( F=161.55 , 172.14 , 289.73; all P=0.00 ) ; the level of serotonin (biomarker for well-being), cortisol (indicator of stress) and HS-CRP (biomarker for inflammation) showed a statistical improvement (F=307.46, 182.85, 102.23; all P=0.00).After 3, 6 and 12 months of regular TCC exercise, according to the results of QOL questionnaire , the indicators including quality of sleep , perceived hunger, fatigue, contentment, stress and social interaction presented a significant difference (F=312.98 , 222.64 , 543.90 ,46.05 ,28.10 ,78.92 , all P<0.05 ) , while there was no statistical difference in life dissatisfaction ( F=56.61 , P=0 .166 ) Conclusions TCC physical activity for post-treatment breast cancer patients improved QOL and overall well-being, leading to improved mental , physical and psychological functioning .Regulated levels of serotonin and cortisol mediated by TCC exercises are proved to be vital for continued good health .

Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glu-cocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly un-derstood. In this study, we cloned the human brain serotonin transporter into Xenopus oocytes, to establish an in vitro expression system. Two-electrode voltage clamp recordings were used to detect serotonin transporter activity. Our results show that mifepristone attenuates serotonin transporter activity by directly inhibiting the serotonin transporter, and suggests that the se-rotonin transporter is a pharmacological target of mifepristone for the treatment of psychotic depression.