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Effect of Chaihu Shugan decoction on gastric smooth muscle cell apoptosis in rats with functional dyspepsia
Objective:To investigate the effect of Chaihu Shugan decoction (CSD) on gastric smooth muscle cells (GSMCs) apoptosis in rats with functional dyspepsia (FD).Methods:48 Sprague Dawley (SD) rats were randomly as signed into six groups:a normal control group,a model group,a positive control (domperidone) group and low-,middle-and high-dose CSD groups.A rat model of FD was established by constantly squeezing their tails.The rats were administered CSD (0.16 g/mL,0.32 g/mL,0.64 g/mL) or domperidone (0.3 g/L) via intragastric gavage for four weeks.The gastric emptying rate was detected at 4 weeks post-administration.Apoptosis of GSMCs was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and the mitochondrial morphology was observed by transmission electron microscopy.The expres sion of Bcl 2 and Bax was measured by immunohistochemistry.Results:FD resulted in marked reduction of gastric emptying rate,severe gastric tissue damage and mitochondria in jury,but were reversed by CSD treatment (P ＜0.05).The apoptosis-induced protein Bax was markedly down-regulated by CSD,whereas the expression of the anti-apoptotic Bcl 2 protein was notably increased (P ＜ 0.05).Furthermore,CSD could protect the FD rats against GSMCs apoptosis manifested by a decreased in TUNEL positive cells (P ＜0.05).Conclusion:CSD could alleviate GSMCs apoptosis in FD rats,possibly by the modulation of Bcl-2 and Bax expression,and the suppression of mitochondria injury.
