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大鼠腹侧前列腺中受氟他胺调控的新基因:去势和氟他胺处理对其表达的调控
Aim: To identify flutamide regulated genes in the rat ventral prostate. Methods: Total RNA from ventral prostates of control and flutamide treated rats were isolated. Differentially expressed transcripts were identified using differential display reverse transcriptase polymerase chain reaction. The effect of castration on the expression of flutamideregulated transcripts was studied. Results: We have identified β2-microglobulin, cytoplasmic FMR1 interacting protein 2 and pumilio 1 as flutamide induced and spermine binding protein and ribophorin Ⅱ as flutamide repressed targets in the rat ventral prostate. Although flutamide treatment caused an induction of pumilio 1 mRNA, castration had no effect. Conclusion: Castration and flutamide treatments exert differential effects on gene expression. Flutamide might also have direct AR independent effects, which might have implications in the emergence of androgen independent prostate cancer and the failure of flutamide therapy.
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血清前列腺特异性抗原在雄激素阻断治疗前列腺癌中的意义
目的探讨血清前列腺特异性抗原(PSA)在雄激素阻断治疗前列腺癌中的作用与意义.方法根据检查结果按Whitmore-Jewett标准进行临床分期:B期3例,C期14例,D期7例,双侧睾丸切除术后1周开始服用Flutamide,剂量为250 mg,每8 h1次.每月复查血清PSA及前列腺体积.结果随访3个月～3年.1个月后PSA水平明显下降;6个月后B超显示前列腺体积明显缩小;2例肺部转移,5个月后复查发现肺部肿块缩小;5例骨转移伴骨痛,1个月后骨痛明显缓解,6个月后ECT及CT显示骨转移灶缩小.结论去势加Flutamide是一种治疗中晚期前列腺癌安全、有效的方法,可根据PSA的水平来调节用药及预后监测.
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Flutamide对前列腺癌细胞分化抑制因子1表达影响
目的 了解雄激素受体阻断剂Flutamide对前列腺癌细胞分化抑制因子1(ID1)表达的影响及意义.方法 在培养的前列腺癌LNCaP和PC3细胞中分别加入Flutamide,用实时荧光定量反转录聚合酶链式反应(RT-PCR)方法检测ID1 mRNA相对定量表达;用Western Blotting方法检测ID1蛋白表达,以不加Flutamide细胞作为对照组.结果 与对照组比较,Flutamide组LNCaP和PC3细胞ID1 mRNA相对定量明显减少,LNCaP细胞中ID1蛋白表达明显减少,差异有显著性(t=3.09～34.91,P＜0.05).Flutamide组LNCaP中ID1 mRNA相对定量、ID1蛋白表达低于PC3细胞,差异有显著性(t=2.57、12.52,P＜0.05).结论 Flutamide能抑制前列腺癌细胞中ID1的表达,ID1的表达可能受雄激素-受体途径的调控.
