Objective:To study Zn influence on NO synthase in rats’ sea-horse after different-time rest after sleep deprivation. Methods:Build SD model of rat by mini-platform-water-environment, indicate the exist-ence of positive structure of NOS by NADH-d, and count all the positive neurons. Results: Compared with CC group, R0h group, R4h group rise significantly(P<0. 01),R12h group decreases significantly(P<0. 01), the rest don’t differ significantly. R0h+Zn group and R4h+Zn group decrease significantly compared with R0h group and R4h group respectively(P<0. 01), R8h+Zn group doesn’t differ from R8h group, R12h+Zn group rises signifi-cantly compared with R12h group(P<0. 01). Conclusions: Zn resists change of activities of NOS after SD, pro-motes recovery of memory.

Ghrelin, a brain-gut peptide that induces anxiety and other abnormal emotions, contributes to the effects of insomnia on emotional behavior. In contrast, the traditional Chinese Medi-cine remedy Wen Dan Tang reduces insomnia-related anxiety, which may perhaps correspond to changes in the brain-gut axis. This suggests a possible relationship between Wen Dan Tang’s pharmacological mechanism and the brain-gut axis. Based on this hypothesis, a sleep-deprived rat model was induced and Wen Dan Tang was administered using oral gavage during model es-tablishment. Wen Dan Tang signiifcantly reduced insomnia-related anxiety and prevented Ghrelin level decreases following sleep deprivation, especially in the hypothalamus. Increased expression of Ghrelin receptor mRNA in the hypothalamus was also observed, suggesting that reduced anxi-ety may be a result of Wen Dan Tang’s regulation of Ghrelin-Ghrelin receptors.

睡眠剥夺在抑郁症治疗中的研究进展

1971年,Pflug 和Tolle对一例伴有严重失眠的抑郁症患者进行了试验性的全睡眠剥夺(total sleep deprivation ,TSD)[1],令人惊奇的是,在睡眠剥夺后的第二天,患者的情绪得到了明显的改善.无论是三环类抗抑郁药还是新型的选择性五羟色胺受体阻滞剂,亦或心理治疗,其起效均至少需要两到三周的时间,甚至更长.有些研究者继续对其进行研究,并提出了一些改进的方案,减少其快速复发率,并对其作用机制进行了初步的研究和探讨.