ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.

CKLFSF2在人精子发生中的表达和定位

Aim: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFPCKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods. Results: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. Conclusion: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.

Various methods are currently under investigation to preserve fertility in males treated with high?dose chemotherapy and radiation for malignant and nonmalignant disorders. Human umbilical cord mesenchymal stem cells(HUC?MSCs), which possess potent immunosuppressive function and secrete various cytokines and growth factors, have the potential clinical applications. As a potential alternative, we investigate whether injection of HUC?MSCs into the interstitial compartment of the testes to promote spermatogenic regeneration efifciently. HUC?MSCs were isolated from different sources of umbilical cords and injected into the interstitial space of one testis from 10 busulfan?treated mice(saline and HEK293cells injections were performed in a separate set of mice) and the other testis remained uninjected. Three weeks after MSCs injection, Relative quantitative reverse transcription polymerase chain reaction was used to identify the expression of 10 of germ cell associated, which are all related to meiosis, demonstrated higher levels of spermatogenic gene expression(2–8 fold) in HUC?MSCs injected testes compared to the contralateral uninjected testes(ifve mice). Protein levels for germ cell?speciifc genes,miwi, vasa and synaptonemal complex protein (Scp3)were also higher in MSC?treated testes compared to injected controls 3weeks after treatment. However, no different expression was detected in saline water and HEK293cells injection control group. We have demonstrated HUC?MSCs could affect mouse germ cell?speciifc genes expression. The results also provide a possibility that the transplanted HUC?MSCs may promote the recovery of spermatogenesis. This study provides further evidence for preclinical therapeutic effects of HUC?MSCs, and explores a new approach to the treatment of azoospermia.

Clinical effectiveness of the various approaches for infertility treatment in women with PCOS and hyperprolactinemia