Purpose: To observe the effects of electroacupuncture therapy on T cells and activity of NK cell in the patient of Chemotherapy. Method: Electro-acupuncture therapy was simultaneously applied during chemotherapy, T cells and activity of NK cell of patients were determined before electroacupuncture treatment (before chemotherapy) and after 4-course electro-acupuncture treatments. Results: Before chemotherapy, CD3 was low within the normal range, CD4 was much lower than the normal range, and CD8, CD4/CD8 and activity of NK cell were within the normal range. After one month of chemotherapy combined with electro-acupuncture, no decline of all the indices was found (P>0.05). Conclusion: Electro-acupuncture can really increase the immune function of patients of chemotherapy.The suppression of both immunity and hematopoiesis caused by chemotherapy is mainly characterized by declines of T cells subpopulations (CD3, CD4, CD8), activity of NK cell, WBC count and humoral immunity. Since 1996, the authors have applied electro-acupuncture therapy for 28 cases of malignant tumor in the process of chemotherapy with good results as reported in the following.

Three-month-old Alzheimer’s disease model transgenic mice were immunized with Aβ1-42, Plp-Adenovirus [Ad]-X-CMV-(Aβ3-10)10-CpG [AdCpG-(Aβ3-10)10] or AdCpG virus lfuid via na-sal mucosal inhalation, respectively. ELISA analysis of serum showed Aβ42 antibody titers were signiifcantly increased in mice immunized with Aβ1-42 and AdCpG-(Aβ3-10)10. Concanavalin A and AdCpG-(Aβ3-10)10 stimulation signiifcantly increased the number of proliferating spleen cells cultured from AdCpG(Aβ3-10)10 and Aβ42 groups compared with the control group. In the AdCp-G(Aβ3-10)10 group, levels of interleukin (IL)-4 and IL-10 were increased, while those of IL-2 and interferon-γwere decreased. In the Aβ42 group, levels of IL-4, IL-10, IL-2 and interferon-γwere all increased. Experimental ifndings indicate that AdCpG-(Aβ3-10)10 vaccine can produce strong T helper 2 (hT2) humoral immune responses in addition to the production of Aβ42 antibody. hTe cellular immunologic response was weak and avoided Aβ1-42-mediated cytotoxicity.

Objective To study the humoral immunity status and distribution pattern of lymphocyte subgroups of peripheral blood mononuclear cell (PBMC) in patients with Kaschin - Beck Disease (KBD), and their relationship with erythrocyte selenium. Methods 23 X- ray diagnosed patients, 22 age- and sex- matched healthy children in KBD affected area (KAA), And 25 in KBD non- affected area (KNAA) were randomly selected. Immunohistochemistry with monoclonal antibodies anti - CD4, anti-CD8. anti - CD20 was conducted to analyze the lymphocyte subsets. Serum IgM, IgA, IgG, Complement C3 and C4 were assayed using rate nephelometry (Array 360 System, USA). The contents of erythrocyte selenium was determined by 2,3 - diaminonaphthalene fluorescence assay. Results CD4+ and CD8 + cells percentage in PBMCs and serum IgA were significantly lower in KAA than those in KNAA( P ＜ 0.05). CD20 +percentage in KAA displayed a decreasing trend compared to KNAA, although not statistically significantly. No statistical differences were found in CD4/CD8 ratio, serum IgG, IgM, C3 and C4 levels. Erythrocyte selenium level in KAA still showed a pronounced decrease compared to that in KNAA. Correlation analysis showed that erythrocyte selenium contents had a strong association with the CD4 cell percentage ( r =0.625, P ＜0.05), and also a close relationship with serum IgA ( r =0.462, P ＜0.05). In addition, we detected a moderate correlation between the serum IgA and CD4 + percentage ( r = 0. 130, P ＞ 0. 05). Conclusion Taken together, our resalts suggested that children in KAA had a comparably low cellular immunity level manifested by the marked depression of CD4 and CD8 cells percentage, and their humoral immunity status was also in a state of moderate immune suppression. Of this immune disorder in KBD patients, selenium deficiency probably played a critical role via affecting the distribution pattern of peripheral blood lymphocyte. Selenium-deficiency and immune impairment maybe both have something to do with the cause - effect chain of KBD.

玉屏风散佐治小儿哮喘的疗效及体液免疫调节的研究

支气管哮喘是儿童常见的慢性呼吸道疾病之一.2000年我国城市儿童哮喘患病率1.54%.由于哮喘病变受各类因素的影响较多,发病机制复杂,治疗困难,发病率在逐年上升.