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合并全身放射损伤伤口表皮再上皮化延迟的机制研究
Objective To investigate the mechanisms of delayed re epithelization in wounds combined with whole body irradiation (WBI) injury.Methods We observed re epithelization in wounds by histological methods and transmission electron microscopy, detected the contents of proliferation cell nuclear antigen (PCNA) protein and mRNA using immunohistochemistry and in situ hybridization (ISH) methods. Results Re epithelization in wounds combined with WBI injury was significantly delayed as compared with simple incision injury, and the contents of PCNA protein and mRNA significantly decreased too. Conclusions WBI injury has direct effect on wound epidermic cells, especially the suppress of epidermic basal cell proliferation, which may be an important reason why wound combined with WBI injury heals more slowly.
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目的:研究血管内皮生长因子(Vascular endothelial growth factor VEGF)对人胚胎视网膜血管发生的调节作用.方法:收集54例9～40周龄胎儿眼球后壁标本,免疫组织化学染色,光镜观察.结果:①VEGF在视网膜的表达呈波峰式分布,高峰在9～13周及26周左右.②节细胞层的梭形细胞(血管内皮细胞前体细胞)、血管内皮细胞呈增殖细胞核抗原(Proliferation cell nucelear antigen,PCNA)免疫反应阳性,水平波动,高峰在9～13周及21周前后,此期间梭形细胞不断增殖、分化形成内皮细胞索,经改建形成视网膜内层血管,26、34周起见内核层内、外缘血管内皮细胞呈PCNA免疫反应阳性,并保持至足月.③视网膜VEGF表达量与梭形细胞、血管内皮细胞PCNA表达量呈显著正相关(r=0.736,P＜0.1).结论:VEGF对视网膜内层及外层血管发生均有促进作用.
