Background:Malignant spinal cord compression has signiifcantly increased hospitalization costs and even with best approach in treatment the disease course remains relatively stable with dire outcomes. Case presentation: The patient was an 80 years old male with the past medical history of hypertension, stroke with chronic right sided weakness, recently diagnosed with non-squamous cell lung carcinoma stage T4N0Mx presently undergoing chemotherapy as outpatient with carboplatin and taxol presented to the emergency room with the chief complaint of right leg pain with weakness and chest pain for 1~2 days. On d 4 of the admission patient complained of chest pain again and a CT angiogram was ordered as part of the work up for chest pain based on high probability for a pulmonary embolus per“Wells Score”. The CT angiogram revealed a large soft tissue mass centered at T5 vertebral body and probable spinal canal invasion. Conclusion:A more favorable outcome requires the input of both a surgeon and a radiation oncologist to ifnd the most effective approach depending on the area involved and the extent of the lesion, and patient’s choice of treatment always must be respected as well. Despite aggressive treatment patient did not respond well and was deteriorating. Options were discussed with the patient, including the futility of care and lack of response. Patient opted to return home with hospice care and was subsequently discharged home with family.

Objective:To explore the effect of ibandronate on the proliferation and the expression of human telomerase reverse transcriptase (hTERT) of non-small cell lung cancer (NSCLC) A549 cell line in vitro. Methods: Methyl thiazolyl tetrazolium (MTT) assay, microscope, flow cytometry (FCM) and semi-quantitative RT-PCR were employed to detect the cell proliferation, cell cycle as well as the morphological change and the expression of hTERT mRNA of A549 cell line. Results:The data showed that ibandronate could effectively inhibit the proliferation of A549 cell line in time-and concentration-dependent. Under the microscope, the lfoating cells increased gradually as the drug concentration increasing. FCM detection showed that ibandronate could induce the cell cycle stopped in G0/G1 phase and downregulation expression of hTERT. Conclusion:Ibandronate can inhibit the proliferation of A549 cell line in vitro, whose mechanism may be associated with cell cycle arrestted in phase G0/G1 and downregulation expression of hTERT.

Objective:To comprehend the characteristics of patients with positive human immunodeifciency virus (HIV) (1+2) antibodies in the preliminary screening so as to provide basis for local HIV screening and prevention. Methods:Enzyme-linked immunosorbent assay (ELISA) was used to detect serum HIV (1 +2) antibodies in the preliminary screening, after which the positive serum was sent to acquired immune deficiency syndrome (AIDS) confirmatory laboratory to be confirmed with western blotting method. Clinical data of patients with positive HIV antibodies in the preliminary screening in the outpatients and inpatients from 2006 to 2013 were collected and analyzed. Results:A total of 394 patients with positive serum HIV antibodies were screened initially, in which 214 were confirmed positive HIV, 13 were not certain and another 167 were negative. Patients with positive serum HIV antibodies in the preliminary screening were increased from 9 cases in 2006 to 94 cases in 2013, in which those conifrmed with positive HIV increased from 5 to 49. Patients with positive serum HIV antibodies in the preliminary screening and those conifrmed increased annually. In addition, patients confirmed with positive serum HIV antibodies were mainly males and aged 20 ~ 49 years, distributing in Departments of Infections, Respiratory, Dermatology, Hematology and Emergency, whereas those confirmed with negative HIV were mainly females and aged >20 years, distributing in Departments of Hematology, Maternity and Emergency as well as Reproductive Center. Conclusion: HIV infection is in low level with characteristics of annually increasing infection rate, male-orientated and wide-spread distribution in variuos departments, etc., knowing which will help guide the clinical practices and carry out the local HIV screening and prevention by relevant departments.

Objective:To explore the expression of ezrin and its signiifcance in non-small cell lung cancer (NSCLC). Methods:The expression of ezrin in 81 NSCLC patients were examined by immunohistochemical staining Envision method, and its relationships with the clinopathologic features and other indexes were analyzed. Results:The high expression rate of ezrin in NSCLC was 59.26% (48/81) and significantly correlated with lymph node metastasis (P < 0.05), but not associated with gender, histologic subtype, differentiation, TNM stages or smoking. The K-M survival analysis demonstrated that patients with the over-expression of ezrin were obviously poorer than those with low-expression of ezrin (P<0.05). Conclusion:The expression of ezrin may be associated with metastasis of NSCLC, can be as an important marker for evaluating the prognosis of NSCLC patients.

Objective:To investigate the clinical value of total knee arthroplasty (TKA) on patients with advanced knee osteoarthritis. Methods:The clinical data and efficacy of 26 patients with advanced knee osteoarthritis (26 knees) who were given TKA in our department from June 2012 to May 2013 were retrospectively observed and analyzed. The knee function scores before operation and after follow up were evaluated according to American HSS scoring standard. Results:At the end of follow up, of the 26 patients, 18 were excellent, 6 were good and 2 were not bad in knee function and mobility without sense of pain, which was regarded to be associated with the poor enthusiasm in knee function training, and the total rate of excellent and good was 92.3%. Conclusion:TKA has signiifcant clinical value and favorable efifcacy on patients with advanced knee osteoarthritis.

Objective:To observe the expressions of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. Methods:Neoplastic tissues and autologous non-neoplastic tissues were taken from 12 patients with colorectal cancer immediately after the operations. The mRNA expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and 11beta-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western-blot assay. Results:Both isoforms of 11β-HSD expressed in the colon adenocarcinoma, but their expressions were not identical in neoplastic and non-neoplastic tissues. There was a significant decrease of 11β-HSD2 mRNA abundance and protein abundance in neoplastic tissue. 11β-HSD1 protein and mRNA abundance were not signiifcantly different from that in control tissue. Conclusions:Neoplastic transformation is associated with the decreases of 11β-HSD2 mRNA and protein.

Objective:To explore the proliferation inhibition and apoptosis-inducing effect of evodiamine in human gastric cancer SGC-7901 cells. Methods:After 48 or 24 h exposure to different concentrations of evodiamine, cell proliferation was analyzed using tetrazolium blue (MTT) assay while apoptosis and cell-cycle phase distribution using lfow cytometry. Results:In 0.01~30.00μg/mL range of concentrations, evodiamine inhibited the proliferation of SGC-7901 cells in dose-dependent manner, and the overall mean IC50 was (3.79±0.16)μg/mL;the apoptosis rate was increased from 3.4%to 7.0%, 13.8%and 36.3%at concentrations of 0, 0.5, 1.5 and 30μg/mL of evodiamine, respectively;the percentage of cells accumulated in G2/M phase was increased from 17.26%to 98.92%in the cells treated with evodiamine for 24 h in 0.01~30.00μg/mL range of concentrations. Conclusion:Evodiamine can inhibit the proliferation, induce apoptosis in human gastric cancer cell line SGC-7901 in vitro and arrest the cell cycle at the G2/M phase.

Objective:To investigate the expressions of AT1-R, AT2-R and angiotensin converting enzyme (ACE) in mesenchymal stem cells (MSCs) under hypoxia and serum deprivation condition. Methods:Bone MSCs were isolated, cultured and identiifed by anti-CD29 and anti-CD11b/c with flow cytometry. The ischemic injury model was established by exposing MSCs to hypoxia and serum deprivation (Hypoxia/SD). Cell viability and apoptotic rate were detected by trypan blue staining, CCK8 assays and Annexin V-FITC staining. The mRNA expressions of AT1-R, AT2-R and ACE were determined by Reverse Transcription-PCR and Real-time Quantitative PCR, The expression of AT1-R, AT2-R and ACE protein were measured by Western-blot. Results:MSCs expressed CD29, but not the CD11b/c. The purity of MSCs employed was up to 97%. The results of trypan blue staining along with CCK8 and Annexin V-FITC staining proved that the injury model induced by Hypoxia/SD was successfully established. MSCs under hypoxia and serum deprivation for 24 h induced a rapid increase in mRNA expression of AT1-R, AT2-R and ACE as well as their protein expressions. Conclusion:The local RAS in MSCs is activated by Hypoxia/SD stimulation and the mRNA and protein expressions of AT1-R, AT2-R and ACE are up-regulated.

Most of cancer patients are suffering from psychological distress which is under-diagnosed and under-treated in China. The purpose of this study is to evaluate the psychometric properties of a new screening instrument for psychological distress in cancer patients based on Chinese culture and personality traits. The scale was created after face-to-face interviews and focus group discussion of 50 medical staffand 30 cancer patients; the analysis of reliability and validity came from 1122 scales completed by cancer patients and non-cancer patients. Data was analyzed for internal consistency reliability, construct validity and discrimination validity in clinical practice. The internal consistency reliability of the scale was 0.906. Principal components factor analysis and structural equation modeling showed that the scale was comprised of four dimensions: depression, anxiety, interpersonal barrier and suspiciousness. The ift indices were χ2(149)=257.594, χ2/d?=1.729, GFI=0.927, NFI=0.846, NNFI=0.906, RMSEA=0.061. These results indicated there were signiifcant differences between cancer patients and non-cancer patients, with respect to the total scores, anxiety scores, interpersonal barrier scores and suspiciousness scores. The psychological distress scale is a new screening instrument for Chinese cancer patients, which is in line with the emphasis of Chinese culture.

Objective: To analyze the clinical features of lung cancer diagnosed by bronchoscopy. Methods:The clinical features of2168 patients with lung cancer diagnosed by bronchoscopy were retrospectively analyzed, including gender, age, pathological type, diseased region, manifestations under bronchoscopy and methods of drawing materials. Results:The ratio of male/female was 4.8:1 and the peak onset age was 60~69 years old. The major pathological type was squamous cell carcinoma (44.5%), then adenocarcinoma (25.9%) and small cell lung cancer (18.3%). The incidence of squamous cell carcinoma was the highest in males (50.6%), while that of adenocarcinoma in females (56.2%). The positive diagnostic rates of forceps biopsy, brush biopsy, bronchial alveolar lavage and transbronchial needle aspiration were 81.6%, 49.4%, 18.2% and 62.6%, respectively, whereas that of biopsy combined with brush biopsy came up to 89.0%. Conclusion: Bronchoscopy is an important method in diagnosis of lung cancer. Different ages and genders of patients with lung cancer have different onset, and the distribution of pathological types is diverse. Attaching more importance to bronchoscopy and improving biopsy technique can signiifcantly improve the diagnostic rate and provide reliable evidences for clinical treatment.