平滑肌肉瘤中PRUNE2mRNA的表达及其与非编码RNA PCA3的一致性关系

目的 探讨平滑肌肉瘤中PRUNE2 mRNA的表达及其与非编码RNA PCA3的关系.方法 采用安捷伦基因表达芯片的方法检测31例平滑肌肉瘤和42例胃肠间质瘤中PRUNE2mRNA的表达水平,分析其表达与平滑肌肉瘤患者的预后关系；采用实时荧光定量聚合酶链反应的方法分析13例平滑肌肉瘤中PCA3mRNA和PRUNE2mRNA的表达水平并分析二者间的关系.7例前列腺癌组织作为PCA3mRNA表达的阳性对照.结果 在31例平滑肌肉瘤中,PRUNE2 mRNA的平均表达水平为368.43 ±29.67,高于37例胃肠间质瘤(82.56±7.45)和7例前列腺癌(4.76±0.98,均P＜0.05).PRUNE2mRNA高表达的平滑肌肉瘤患者的生存期与低表达者比较,差异无统计学意义(X2=0.96,P =0.326).PRUNE2mRNA高表达的胃肠间质瘤患者的生存期与低表达者比较,差异无统计学意义(x2=3.10,P=0.078).平滑肌肉瘤中PCA3 mRNA平均表达水平为26.38±3.04,低于前列腺癌(3272.00±240.87,P=0.004).在平滑肌肉瘤中,PCA3 mRNA与PRUNE2mRNA的表达呈正相关(rs=0.901,P＜0.001).结论 PRUNE2的表达与平滑肌肉瘤患者的生存期有关,PCA3的表达与PRUNE2 mRNA的表达呈正相关.

PRUNE2 plays an important role in regulating tumor celldifferentiation, proliferation, and invasiveness in neuroblastoma. Our previous study revealed that PRUNE2/OBSCN two-gene relative expression classifer accurately differentiated leiomyosarcoma from gastrointestinal stromal tumor. However, the association between PRUNE2 expression and prognosis in leiomyosarcoma is poorly understood. In this study, we evaluated the prognostic role of PRUNE2 in leiomyosarcoma. PRUNE2 expression was detected using immunohistochemistry in 30 formalin-fixed, paraffin-embedded leiomyosarcoma tissues from MD Anderson Cancer Center, and high expression was detected in 36.7%(11/30) of the samples. To validate these results, immunohistochemistry was performed on another cohort of 45 formalin-fixed, paraffin-embedded leiomyosarcoma tissues from Tianjin Medical University Cancer Institute & Hospital, and high PRUNE2 protein expression was detected in 37.8%(17/45) of the samples. Moreover, elevated PRUNE2 expression was significantly associated with tumor size (P = 0.03) and hemorrhage/cyst (P = 0.014), and was an independent favorable prognostic factor for overal survival in leiomyosarcoma patients from Tianjin Medical University Cancer Institute & Hospital (P < 0.05). These data suggest that increased PRUNE2 protein expression may serve as a favorable prognostic marker in human leiomyosarcoma.

LncRNA PCA3在甲基丙烯酸环氧丙酯诱导16HBE恶性转化细胞中的表达及意义

目的:探讨长链非编码RNA PCA3(LncRNA PCA3)在甲基丙烯酸环氧丙酯(GMA)诱导的16HBE恶性转化细胞中的表达水平及意义.方法:收获经8 μg/mL GMA诱导的第30代16HBE恶性转化细胞及同代龄DMSO溶剂对照组细胞,应用高通量LncRNA芯片比较两组样本表达谱的差异,通过差异倍数、邻近编码基因信息分析等策略初步筛选出16HBE恶性转化细胞中LncRNA PCA3及其可能的相关蛋白编码基因PR UNE2,采用实时荧光定量PCR(qPCR)和全基因组表达谱芯片分析LncRNA PCA3和PRUNE2的表达量,并与同代龄DMSO对照组细胞比较.结果:LncRNA芯片结果显示,与同代龄DMSO组相比,GMA诱导的16HBE恶性转化细胞中LncRNA PCA3上调7.17倍,PRUNE2下调2.54倍;qPCR结果显示,与同代龄DMSO组[(1.36±0.44)×10-5]相比,GMA诱导的恶性转化细胞[(2.67±0.63)×10-5]中LncRNA PCA3表达上调(P＜0.05);全基因组表达谱芯片显示,16HBE恶性转化细胞的PRUNE2表达量(10.95)较DMSO组(19.46)明显下调,与LncRNA芯片结果一致.结论:LncRNA PCA3可作为GMA诱导的16HBE恶性转化细胞中相关特异分子标志之一.