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早期乳腺癌的辅助治疗策略(视频)
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银屑病患者皮肤屏障功能受损的研究
目的 探讨银屑病患者皮肤屏障功能受损的实验依据,以指导临床辅助治疗银屑病.方法 60例银屑病患者运用无创性皮肤生理功能测试仪检测皮损角质层含水量、皮脂含量及经表皮水分流失(TEWL).电镜观察皮损处细胞超微结构,同时运用免疫组化方法检测皮损处酸性神经酰胺酶的表达.结果 与正常皮肤比较,银屑病皮损角质层含水量降低(P<0.01),TEWL值增加(P<0.01),皮脂含量差异无统计学意义.电镜下,皮损颗粒层角质形成细胞中板层小体数量较正常对照组减少,分布紊乱,体积大小不等;免疫组化染色显示酸性神经酰胺酶表达明显减少.结论 银屑病皮肤屏障功能明显受损,因此,恢复皮肤屏障功能,加强保湿是银屑病重要的辅助治疗手段之一.
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早期乳腺癌全身辅助治疗的心脏副作用
虽然手术治疗仍是早期乳腺癌的主要治疗手段,但是在很多患者中手术无法清除肿瘤的微转移灶.一旦肿瘤发生内脏的转移,就很难被治愈.为了减少乳腺癌远处转移的危险性,术后在合适的患者中应用全身性的辅助化疗和(或)内分泌治疗是非常必要的.大规模的随机临床试验已证实,全身辅助治疗能延长乳腺癌患者的生命.早期乳腺癌临床试验协作组(EBCTCG)的新荟萃分析显示,5年疗程的三苯氧胺能使乳腺癌的年复发危险性降低40%,而多药联合化疗则能使其降低24%[1].
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于福興實驗室研究計劃
1 Molecular regulation of corneal wound healing Rapid healing of the corneal epithelium in response to injury is essential for maintenance of its barrier function. The long-term goal of this project is to obtain basic information about the molecular and cell biology of corneal wound healing. The project will test the hypotheses that amyloid β/A4 precursor-like protein controls serine proteinase activity, mediates cell adhesion, and promotes cell migration during corneal reepithelialization. This study should provide the basis to begin constructing a detailed nap of the molecular pathways and interconnecting networks of proteins functioning in wound repair and to develop therapeutics for treatment of corneal diseases like recurrent erosions and persistent defects of the epithelium.2 Developing an ex vivo model for ocular irritation testThe objective of this project is to develop an ex vivo assay system to predict ocular irritation potential of test chemicals and consumer products. Our hypothesis has been that activation of these transcription factors and disruption of corneal integrity can be used as endpoints/ markers for evaluating ocular toxicity in organ culture. Our goal is to develop a sensitive, efficient, economical and reliable ex vivo model for predicting irritation potential of a chemical or consumer product with mechanistic basis.3 Modulation of epithelial barrier function during corneal infectionThe long-term goal of this project is to understand the mechanisms underlying the induction of the inflammatory reaction and breakdown of the epithelial barrier in the cornea upon infection. We will test the hypothesis that in the cornea TLRs confer responsiveness of HCE cells to pathogens, and PA challenge-induced TLR signaling, through activation of NF-?B and/ or mitogen-activated protein kinase (MAPK), contributes to infection-induced epithelial barrier breakdown. The following studies will be carried out. An understanding of how TLRs transmit signals that lead to epithelial response, including modulation of barrier function,may allow the development of therapeutic agents that prevent breakdown or enhance recovery of barrier function during infection and, as an adjuvant therapy, eliminate the corneal scarring and vision loss associated with bacterial keratitis.4 Developing an adjuvant therapy to reduce inflammatory response induced by bacterial infection of the cornea (bacterial keratitis).
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pGEX-e23 (scFv) PE40融合基因的构建及其在大肠杆菌中的表达
目的 在大肠杆菌中表达抗原癌基因c-erbB-2表达产物p185的单 链抗体e23(scFv)/假单孢菌属外毒素活性片 段PE40免疫毒素的融合蛋白,为乳腺癌、胃癌等多种c-erbB-2呈过量表达的恶性肿瘤的免疫治疗奠定基础. 方法 去除克隆在真核表达载体pLNCX中的e23 (scFv) PE40基因5′端编码信号肽的核苷酸序列,并将改建后的融合基因克隆到原核融合表达载体pGEX-4T中表达. 结果 序列测 定 表明. 改建后的抗p185 e23(scFv)/PE40序列正确. 融合基因经IPTG诱导表达4 h后,经SDS -聚丙烯酰胺凝胶电泳分析,在Mr 90 000处出现一条新生蛋白带,表达量约占菌体总蛋白的15%. 结论 成功改建并在原核中表达了抗p185 e23 (scFv)/ PE40融合蛋白.
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The Current Adjuvant Therapies for the Glioblastoma multiforme: Chemotherapy, Targeted Molecular Therapy and Immunogene Therapy