原发性肝癌患者T淋巴细胞亚群变化及其与CTCs、TTV及HBV-DNA的关系

Objective To investigate the changes of T cell immune function in patients with hepatocellu-lar carcinoma(HCC)and its relationship with circulating tumor cells (CTCs), total tumor volume (TTV) and hepatitis B virus (HBV-DNA). Methods Peripheral blood of 45 patients with HCC was collected， and pe-ripheral blood of 45 normal adults was taken as control. CD3+T cells, CD4+T cells and CD4+/CD8+ratio were determined by flow cytometry. CTCs in the blood were detected by CanPatrolTM. The maximum diameter of tu-mor, quantitative hepatitis B virus (HBV-DNA) and other clinical pathological parameters were collected. The HCC patients were divided into high level group and low level group according to the rate of CD4+/CD8+, CTCs, TTV and HBV-DNA level. Results Compared with the healthy control, the percentage of CD3+T cells, CD4+T cells and the rate of CD4+/CD8+in HCC patients were significantly decreased(P<0.05);CD8+T cells were significantly higher than the normal group (P<0.05). The total CTCs and mesenchymal CTCs in high CD4+/CD8+group were significantly less than in the low CD4+/CD8+group, but there was no significant differ-ence between the two groups of epithelial type CTCs and mixed CTCs (P>0.05). The CD4+/CD8+ratio was positively correlated with TTV (P<0.05), and negatively correlated with the percentage of CD8+T cells (P<0.05). Between different HBV-DNA groups, no statistically significant differences were found between the ra-tio of CD4+/CD8+(P>0.05). Conclusions The immune function of T cells in patients with HCC was disor-dered, and the immune function of T cells was significantly decreased. The immune function of T cell had a close relationship with CTCs and TTV in patients with HCC.