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Effects of GLP on Intestinal Mucosal Injury and the Change of TNF-αContent in Hemorrhagic Shock Rabbits
Objective To observe the intestinal mucosal injury and the change of TNF-αcontent in rabbits with hemor-rhagic shock / reperfusion ( HS-R) and the effects of ganoderma Lcidum polysaccharide ( GLP) on them. Methods 30 rabbits were made into hemorrhagic shock, then reperfused with different liquids. These rabbits were divided by random number table into three groups:sham operation group ( Sham group) , reperfusion with normal saline group ( NS group) , reperfusion with 1% GLP group ( LS group) . Bacterial translocation of blood and TNF-αcontent in serum were respectively observed at the time before shock, 40 min after shock, 40 min and 90 min after. TNF-α content in intestinal mucosa and the degree of intestinal mucosal injury were examined at 90 min post-resuscitation. Results ① With the extension of reperfusion time, the positive rate of blood bacteria increased gradually in NS group, which was significantly higher than that of Sham group and LS group (P<0. 05), meanwhile the degree of intestinal mucosal injury in NS group was more se-vere than that of Sham group and LS group too (P<0. 05). ②TNF-αcontent in serum of NS group and LS group were in-creased obviously compared with that before shock and in Sham group (P<0. 05). TNF-αcontent in serum was further in-creased after reperfusion with NS, which was distinctly higher than that in LS group. TNF-αcontent in intestinal mucosa of NS group was significantly higher than that in LS group and Sham group too (P<0. 05). Conclusion GLP can protect in-testinal mucosa against HS-R injury, and its effects may relate with the change of TNF-αin hemorrhagic shock rabbits.
