Objective:To study effects of the exogenous bone mesenchymalstem cells ( BMSCs) transplanta-tion on silicosis fibrosisin rats, and to explore dose-effect relationship. Methods:BMSCs were isolated and cul-tured from male 5-week-old SD rats in vitro. Fifty healthy female SD rats were randomly divided into five groups:model control group, silicosis model group, BMSCs treatment A group (1x106/ ml), BMSCs treatment B group (3 x106/ ml) , BMSCs treatment C group (5x106/ ml)(n=10). The silicosis model were made by One-time infu-sion of silica dust suspension, using the non-exposed tracheal intubation, and given different doses of BMSCs as intervention therapy. All the rats were sacrificed on the 21st day after model estalishment. The morphological chan-ges of lung tissue were observed by HE staining and Masson staining; the localization and distribution of TNF-αand TGF-β were detected by immunohistochemistry; the expression quantity of TNF-α, TGF-β, collagen I and collagen III protein were detected by Western blotting;the sex-determining gene SRY was searched by immunoflu-orescence to confirme BMSCs home to damage lung tissue. Results: Compared with the model control group, the silicosis model group had significantly alveolitis changes, silicon nodule formation, collagen deposition and other pathological changes. Compared with silicosis model group, the pathological changes above in BMSCs treatment A group were improved;the condition of BMSCs treatment B group was improved significantly;the pathological chan-ges of BMSCs treatment C group were increased obviously. the expression of TNF-α, TGF-β, collagen I and col-lagen III protein in lung tissue:BMSCs treatment C group>silicosis model group>BMSCs treatment A group>BM-SCs treatment B group>model control group. The difference of BMSCs treatment C group and silicosis model group had no statistically significant ( P>0 . 05 ) , and the difference between the other groups had statistically significant 158 (P<0. 05). The SRY-positive cells were observed in BMSCs treatment B group by laser scanning confocal microscope, but no significant expression in the heart, liver, spleen and kidney tissues. Conclusions: The Exogenous BMSCs transplantation antagonize rat silicosis fibrosis, which has dose-effect relationship. Our data suggested that the best transplantation dose for silicosis fibrosis is 3x106 / ml.

MMP-9、Collagen III 在胎膜组织中的表达及意义

目的：探讨基质金属蛋白酶-9（ MMP-9）、Collagen III在胎膜组织中的表达及其与胎膜早破之间的关系。方法应用免疫组化分别检测20例足月胎膜早破（tPROM）、20例未足月胎膜早破（pPROM）、10例早产和10例正常足月分娩患者胎膜组织标本中MMP-9、Collagen III 的表达，分析其相关性及其与胎膜早破的关系。结果胎膜早破组胎膜组织中，MMP-9的阳性表达率为90％，高于对照组（P＜0．01）。胎膜早破组CLO-Ⅲ的阳性表达率为30％，低于对照组P＜0．05），MMP-9的表达与Collagen III的表达呈负相关（r＝-0.612，P＜0．05）。结论 MMP-9的高表达及Collagen III的减少可能与胎膜早破的发生有关。