电针对新生脑性麻痹大鼠单胺类神经递质的影响

objective to detect the content of monoamine neurotransmitters in hippocampus, cortex, basal nucleus and the brainstem of newborn rats with cerebral palsy, to observe the effects of acupuncture on monoamine neurotransmitters, and to explore the mechanism of acupuncture treatment to newborn rats with cerebral palsy. Methods using the method of hypoxic ischemic to make the model of newborn rats with cerebral palsy,decapitating brain after giving acupuncture treatment, separating the required brain regions, and detecting the content of monoamine neurotransmitters with speetrophotometer.Results Compared with the sham operation group, the content of dopamine (DA), 5-Hydroxytryptamine (5-HT) in basal nucleus area of newborn rats with cerebral palsy, and norepinephrine (NE) in Brainstem area decreased significantly(P<0.05), which elevated ignificantly(P<0.05)afler acupuncture treatment. Conclusion it indicated that to improve the ontent of monoamine neurotransmitters in related brain areas may be one of the mechanisms in the acupuncture treatment of newborn rats with cerebral paraly.

Objective:To study the effect of ketamine combined with fluoxetine on behavior indexes and related gene expression in depression rat model.Methods:SD rats were used as experimental animals and randomly divided into control group (C group), model group (M group), ketamine group (K group), fluoxetine group (F group) and ketamine combined with fluoxetine group (KF group); chronic unpredictable stress depression models were built and different medications were given. Then behavior indicators were detected by tail suspension test and open field test; contents of monoamine neurotransmitters were determined by HPLC-electrochemical detection assay; mRNA contents of monoamine neurotransmitter-metabolizing enzymes, BDNF and its receptor were detected by PCR method.Results: (1)behavior indexes: compared with M group, behavior indexes of K group, F group and KF group were all improved; tail suspension immobility time and central grid staying time of KF group were shorter than those of K group and F group; squares crossed number, standing up number and decoration number were more than those of K group and F group; (2) molecular indexes: compared with M group, molecular markers of K group, F group and KF group were all improved; NE, 5-HT, TH, TPH, BDNF and TrkB contents in hippocampal and prefrontal cortex tissue of KF group were higher than those of K group and F group.Conclusion:Ketamine combined with fluoxetine therapy can more effectively reduce depression-related behavior; its mechanism may be related to the regulation of monoamine neurotransmitter metabolism and brain-derived neurotrophic factor expression in hippocampus and prefrontal cortex.