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CDK4,pRB和E2F1参与人参皂苷Rg1对淀粉样β蛋白诱导的大鼠皮层神经元凋亡的抑制作用
AIM: To explore the possible mechanism of β-amyloid (Aβ)-induced apoptosis in rat cortical neurons and the protective effect of ginsenoside Rg1. METHODS: AO-EB staining was used to quantify the apoptotic cells. DNA fragmentation was observed by gel electrophoresis. The levels of cyclin-dependent kinases-4 (CDK4) and phosphorylated pRB were detected by Western blot. RT-PCR was used to examine the expression of E2F1 mRNA. RESULTS: Treatment with A1-40 at the concentration of 20, 40, 80 mg/L for 48 h induced rat cortical neuron apoptosis from 12.5 %+1.5 % (control) to 22.3 %+1.4 %, 38.8 %+1.3 %, 36.7 %+1.4 %, respectively. Pretreat ment with Rg1 at the dose of 0.5, 1, 2, 4, 8, 16 μmol/L for 24 h, then treatment with A~-40 40 mg/L for 24 h, the percentage of apoptotic neurons decreased from 38.8 %+1.3 % to 14.5 %+1.3 %, 13.3 %+1.0 %, 11.6 %+0.29 %, 11.8 %+ 1.0 %, 6.2 %+0.8 %, 5.8 %+0.8 %, respectively. After treatment with Aβ1-40 40 mg/L for 24 h, there were transient increases in CDK4 and phosphorylated pRB protein level, as well as the expression of E2F1 mRNA. However, the above levels decreased markedly after pretreatment with Rg1 8 μmol/L for 24 h. CONCLUSION: Ginsenoside Rg1 attenuated Aβ1-40-induced apoptosis in rat cortical neurons via inhibiting the activity of CDK4, decreasing the phosphorylation of pRB and downregulating the expression of E2F1 mRNA.
