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Brucellosis is a zoonosis caused by Brucella ,with an acute or chronic clinical infection .Clinical manifestations of brucellosis are various or atypical ,and it is easily misdiagnosed and miss-diagnosed .The case we have reported had an onset of fever and abdominal pain ,associated with arthralgia ,headache and rashes during the course , and was initially misdiagnosed as “acute cholangitis , associated with biliary pancreatitis” . According to epidemiologic survey , radiological evidence , as well as blood culture and agglutination test showing Brucella positive ,the diagnosis of brucellosis associated with splenic infarction and multi-systemic involvement including lungs , skin and brain was made . After regular anti-brucellosis treatment , the patient’s symptoms were significantly improved . Brucellosis associated with splenic infarction is rare ,which is possibly due to vasculitis resulting from Brucella infection .