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Critical reviews in food science and nutrition 实时荧光定量PCR检测加工食品中坚果过敏原的应用。
影响因子:11.208 DOI:10.1080/10408398.2018.1557103
作者: Linacero R,Sanchiz A,Ballesteros I,Cuadrado C 发表时间:2020-07-11 01:06:33
keywords: Linacero RSanchiz ABallesteros ICuadrado C
关键词: 实时PCR 过敏原检测 压力处理 加工食品 热加工 树坚果
Abstract
:Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
摘 要
: 目前,食物过敏是全球范围内的重要健康问题。食品加工过程中由于意外污染导致的未申报致敏成分的存在或微量过敏原的存在对致敏个体构成了极大的健康风险。因此,需要可靠的分析方法来检测和鉴定食品中的致敏成分。Real-time PCR允许特异性和准确的过敏原序列扩增。一些处理方法可以诱导基因组DNA的断裂和/或降解,一些研究已经进行了分析处理对不同靶标检测的影响,如热处理,有压力和不施加压力。在这篇综述中,我们对实时PCR检测加工食品中坚果过敏原的应用进行了更新概述。还回顾和讨论了对过敏原检测PCR方法学性能有贡献的不同变量。
期刊介绍
《CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION》 (点击进入期刊详情)
英文简介 : The objective of Critical Reviews in Food Science and Nutrition is to present critical viewpoints of current technology, food science, and human nutrition. The application of scientific discoveries and the acquisition of knowledge, as they relate to nutrition, are thoroughly addressed in this comprehensive and authoritative information source.
中文简介 : (来自Google、百度翻译) 食品科学与营养评论的目的是提出当前技术、食品科学和人类营养的批评观点。在这一全面和权威的资料来源中,对科学发现的应用和与营养有关的知识的获取进行了全面的论述。
CIRCULATION RESEARCH 期刊中科院评价数据
新中科院分区
大类(学科) 小类(学科) 学科排名
医学

FOOD SCIENCE & TECHNOLOGY (食品科技)

NUTRITION & DIETETICS (营养学)

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总被引频次 :12591

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