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Abstract:
:Motor neuron disease (MND) is a kind of common clinical nervous system disease with typical characteristic of progressive motor neurons degeneration or death. Motor neuron derived from stem cells or motor neuron progenitor cells will be a good choice to be used for treatment of the disease. In this study, we used the combination of 5 small molecular including CHIR99021 (CHIR), SB431542 (SB), DMH1 (DMH), retinoic acid (RA) and Purmorphamine (Pur) to induce hair follicles neural crest stem cells (hfNCSCs) to motor neurons progenitors (MNPs). Valproic acid (VPA) was used to make MNPs proliferation. RA and Pur were used to try to induce MNPs toward motor neurons (MNs) and CpdE was tried for MNs maturation. Nestin, β-tubulin Ш (Tuj1), microtubule associated protein 2 (MAP2), Olig2, choline acetyltransferase (ChAT)and TUBB3 were examined at protein and mRNA levels by immunofluoresence cytochemistry, western blot and real time PCR at 6, 16 and 22 days. Our data showed cells changed into bipolar or multipolar shape forming the cell clusters like scattered rosettes. Nestin expression decreased significantly at 22 days. Compared to 6 days, percentage of Olig2 + MNPs was higher, (88.53 ± 6.67)%, and Olig2 expression at protein and gene level was lower at 22 days. Percentage of MAP2 positive cells increased to (90.62 ± 2.31) % and ChAT positive cells increased to (83.29 ± 6.62) % at 22 days. It indicates that these 5 molecular can differentiate hfNCSCs into Olig2 positive cells with a unipotent differentiation toward motor neurons.
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最新影响因子:3.097 | 期刊ISSN:0891-0618 | CiteScore:2.07 |
出版周期:Bimonthly | 是否OA:YES | 出版年份:1988 |
期刊官方网址:http://www.journals.elsevier.com/journal-of-chemical-neuroanatomy/
期刊投稿地址:https://www.evise.com/evise/faces/pages/login/login.jspx?resourceUrl=%2Ffaces%2Fpages%2Fnavigation%2
自引率:4.30% | 研究方向:医学-神经科学 |
出版地区:NETHERLANDS |
SCI期刊coverage:Science Citation Index Expanded(科学引文索引扩展)
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Journal of Chemical Neuroanatomy is a vehicle for the publication of scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches.Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples.Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.Journal of Chemical Neuroanatomy will consider for publication manuscripts of an interdisciplinary nature rather than those dealing solely with one area of interest. Two types of contributions will be considered:1. Original research articles: these should contain an Abstract, Introduction, Materials and methods, Results and Discussion sections. The text should be succinct but there is no restriction on the number of pages or figures.2. Review articles: these should extensively survey a specific field. An Abstract is required although Materials and methods and Results sections are not. There are no restrictions on the number of figures or pages. Although review articles are usually invited, submitted review articles will also be considered.The table of contents for this journal is now available pre-publication, via e-mail, as part of the free ContentsDirect service from Elsevier Science. Please send an e-mail message to cdhelp@elsevier.co.uk for further information about this service.
《化学神经解剖学杂志》是一份发表神经系统功能和生化方面与其显微解剖组织相关的科学报告的刊物。该杂志的范围集中于结合显微解剖学、生化、药理学和行为方法的报告。论文应该提供神经系统(特别是大脑和脊髓)的形态学与其生物化学相关的原始数据。《化学神经解剖学杂志》特别感兴趣的是发表使用最新方法进行的重要研究,这些方法包括敏感的化学微分析、杂交瘤技术、免疫细胞化学、原位杂交和受体放射自显影等。化学神经解剖学杂志是利用这些方法进行综合研究的天然载体。这些文章将由编委会根据它们的卓越表现和对神经科学领域的潜在贡献,选择并邀请审稿人。化学神经解剖学的体内和体外综合研究是该杂志感兴趣的合适课题。这些研究应该只涉及脊椎动物物种,特别强调哺乳动物和灵长类动物的神经系统。化学神经解剖学杂志将考虑出版跨学科性质的手稿,而不是那些只涉及一个领域的兴趣。将审议两类捐款:1。原始研究论文:包括摘要、引言、材料和方法、结果和讨论部分。文章应该简洁,但是页数和字数没有限制。复习文章:这些文章应该广泛地调查一个特定的领域。虽然材料、方法和结果部分不需要摘要,但是需要摘要。没有数字或页数的限制。虽然通常会邀请评审文章,但提交的评审文章也会被考虑。该杂志的目录现在可以通过电子邮件预先发布,作为爱思唯尔科学免费内容直接服务的一部分。请发送电子邮件到cdhelp@elsevier.co。有关这项服务的更多信息,请访问英国。
大类(学科) | 小类(学科) | 学科排名 |
医学 |
BIOCHEMISTRY & MOLECULAR BIOLOGY (生化与分子生物学) 4区 NEUROSCIENCES (神经科学) 4区 |
205/293 187/261 |
年度总发文量 | 年度论文发表量 | 年度综述发表量 |
63 | 54 | 9 |
引文计数(2018)
文献(2015-2017)
373次引用
180篇文献
序号 | 类别 | 排名 | 百分位 |
1 |
大类(学科):Neuroscience
小类(学科):Cellular and Molecular Neuroscience
|
#66/85
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JOURNAL OF CHEMICAL NEUROANATOMY 投稿经验
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