
发表一篇学和医学成像类SCI论文
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Abstract:
AIMS:Glycoprotein VI (GPVI) is an important platelet membrane receptor. The expression of GPVI on platelet membranes is increased in patients with coronary heart disease (CHD). DNA methylation is one of the most common post-replication and pre-transcriptional modifications and plays a critical role in the regulation of gene expression. Here, we aimed to reveal how methylation regulates GPVI expression. MAIN METHODS:Pyrosequencing was used to determine whether the GPVI promoter region in leukocytes from CHD patients is hypomethylated. The expressions of GPVI in CHD patients were detected using qRT-PCR and Western blot. The effect of methylation of the GPVI promoter region on regulating its transcriptional activity was analyzed using in vitro luciferase assay. The expression of P-selectin in platelet-like particles was determined using flow cytometry, and SYK phosphorylation was observed using Western blot. KEY FINDINGS:We found that the GPVI promoter region in leukocytes from CHD patients was hypomethylated and the expression of GPVI at the mRNA and protein level was elevated in CHD patients. We also found that the hypermethylation of GPVI promoter region inhibited the expression of GPVI in the -322 to +75, -539 to +75, and -937 to +75 regions in Dami cells. Moreover, the data showed that the methylation or demethylation regulated the GPVI expression and platelet-like particle activation in Dami cells. SIGNIFICANCE:Taken together, these results indicate that DNA methylation regulates GPVI expression and that CpG methylation levels in the promoter region of the GPVI gene may be a biomarker of CHD.
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最新影响因子:6.78 | 期刊ISSN:0024-3205 | CiteScore:3.17 |
出版周期:Weekly | 是否OA:YES | 出版年份:1973 |
期刊官方网址:http://www.elsevier.com/wps/find/journaldescription.cws_home/525477/description
期刊投稿地址:http://ees.elsevier.com/lfs/
自引率:2.00% | 研究方向:医学-药学 |
出版地区:ENGLAND |
SCI期刊coverage:Science Citation Index Expanded(科学引文索引扩展)
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Life Sciences is an international weekly journal publishing reports on research in biomedical areas. Articles are encouraged that emphasize the molecular, cellular and functional aspects of Cardiovascular & Autonomic Mechanisms; Drug Metabolism; Endocrinology; Growth Factors and Neoplasia; Immunology; Neuroscience and Toxicology. The Journal publishes original research rapidly. Although full-length manuscripts are favored, shorter submissions are also considered. These shorter articles must be of the same high quality as full-length articles, and should contain information from topical and fast-moving fields in order to merit more rapid communication. Mini-reviews on topics of wide interest to investigators in the life sciences are also published. All articles are rigorously reviewed. The Journal favors publication of papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research.
《生命科学》是一份国际周刊,出版生物医学领域的研究报告。鼓励强调心血管和自主机制的分子、细胞和功能方面的文章;药物代谢;内分泌学;生长因子与肿瘤;免疫学;神经学和毒理学。该杂志迅速发表原创研究。虽然全篇幅的稿件更受欢迎,但也可以考虑提交较短的稿件。这些较短的文章必须与完整的文章具有相同的高质量,并且应该包含来自主题和快速移动领域的信息,以便进行更快速的交流。对生命科学研究人员广泛感兴趣的主题也发表了小型评论。所有文章都经过严格审查。该杂志倾向于发表现代科学技术用于解释分子、细胞和生理机制的论文。仅仅报告观察结果的文章很少被接受。必须遵守《赫尔辛基宣言》或国家卫生研究院关于护理和使用实验室动物的指导方针的建议。文章应该写在对文章主题本身不是专家,但对研究感兴趣的读者能够理解的水平上。
大类(学科) | 小类(学科) | 学科排名 |
医学 |
MEDICINE, RESEARCH & EXPERIMENTAL (医学:研究与实验) 3区 PHARMACOLOGY & PHARMACY (药学) 3区 |
53/133 78/261 |
年度总发文量 | 年度论文发表量 | 年度综述发表量 |
337 | 287 | 50 |
引文计数(2018)
文献(2015-2017)
4020次引用
1267篇文献
序号 | 类别 | 排名 | 百分位 |
1 |
大类(学科):Biochemistry, Genetics and Molecular Biology
小类(学科):General Biochemistry, Genetics and Molecular Biology
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#41/187
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2 |
大类(学科):Pharmacology, Toxicology and Pharmaceutics
小类(学科):General Pharmacology, Toxicology and Pharmaceutics
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#4/68
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