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糖尿病大鼠心肌磷酸兰苯蛋白含量的改变
目的:观察糖尿病大鼠心肌肌质网 Ca2+-ATP酶(SERCA)和磷酸兰苯蛋白(PLB)的改变.方法:复制糖尿病大鼠模型,12周后比较糖尿病大鼠和对照组大鼠的心脏/体重比,并以Western印迹法测定两组大鼠心肌组织SERCA和PLB含量.结果:糖尿病大鼠心脏/体重比显著大于对照组(P＜0.01);虽然SERCA在两组间无显著差异,但是糖尿病大鼠心肌内PLB表达以及PLB/SERCA比值显著高于对照组(P＜0.05).结论:糖尿病可造成大鼠心肌内PLB含量和PLB/SERCA比值增高.
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Objective: To examine the function change of myocardial calcium transports and determined what role the change plays in cardiac dysfunction after severe burn injury in rats.Methods: The contraction and relaxation properties of the left ventricle (LV) were studied in the isolated hearts preparations of Wistar rats at 3, 8, and 24 h after a 30%TBSA (total body surface area) full-thickness burn. The calcium transport function of the sarcoplasmic reticulum (SR) was measured by the millipore filtration technique.Results: The maximal rate of LV pressure (± dp/dtmax) of the burn group was significantly lower than that of the control group (P ＜ 0.01). In addition, the calciumdependent ATPase activity and the coupling ratio of SR were also markedly depressed.Conclusions: It indicates that the decrease in the SR calcium transport function is one of the important mechanisms for the cardiac contractile dysfunction after severe burn injury.
