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Fast Growth of Eucommia Antifungal Protein(EAFP) Crystals Observed by Atomic Force Microscopy
The eucommia antifungal protein (briefly named as EAFP) is an antifungal protein extracted from the barkes of the eucommia ulmoides Oliv[1]. It is involved in the defense mechanism of plants and plays an important role against the pathogenic attacks. EAFP has 41 residues distinctly with five disulfide bridges. It has been crystallized in a monoclinic form( P21 ) with the unit cell parameters a = 19. 085, b = 23. 225, c = 30. 854?, β = 98.64[2]. EAFP crystals grow much faster than most of other macromolecular crystals. It takes only several hours to grow perfect crystals big enough for x - ray diffraction at very high resolution( about 0.8?). Therefore it is very interesting to know the mechanism of the fast growth process of EAFP crystals.Here we report step growth rates measured from images by in situ Atomic Force Microscopy(AFM).
