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pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价
Objective To provide morphological evidence for therapeutic effects of microgene pSVPoMcat implanted to modify schwann cells(SC) on the experimental spinal cord injury(SCI).Methods Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically SC(group A),SC implanted group(group B) and the control group(group C),each groups having 10 animals.Acetylcholinesterase(AchE) and acid phosphatase(ACP)activity of anterior horn cells in the A,B,C groups were measured respectively with the quantitative enzyme histochemistry ;The number of axon in the spinal white matter was surveyed in the three groups by means of the quantitative immunohitochemistry. Results (1)AchE activity of anterior horn cells in the A,B,C groups were A< B< C at various time,and ACP activity were A >B >C;(2)The number of axon in a certain area of spinal white matter in the A,B,C were A >B >C.Conclusion The therapeutic effects of microgene pSVPoMcat implanted to modify SC on the experimental SCI have the reliable morphological evidence.
