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中国医学科学(英文版)

中国医学科学(英文版)杂志

Chinese Medical Sciences Journal 중국의학과학잡지(영문판)

  • 主管单位: 卫生部
  • 主办单位: 中国医学科学院,中国协和医科大学
  • 影响因子: 0.53
  • 审稿时间:
  • 国际刊号: 1001-9294
  • 国内刊号: 11-2752/R
  • 发行周期:
  • 邮发:
  • 曾用名: 中国医学科学院学报(英文版)
  • 创刊时间: 1986
  • 语言: 英文
  • 编辑单位: 中国医学科学院学报编辑委员会编辑
  • 出版地区:
  • 主编: 刘德培
  • 类 别:
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  • 作者:赵阳青;詹美云

    Objective.To study the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1 (1- 42) and the Core (1- 144) antigen of HBV in E.coli.Methods.The genes of HBcAg (1- 144) and preS1 (1- 42) were amplified and fused by PCR.This fused gene was inserted in the prokaryotic expression vector pET 11d and expressed in E.coli.Results.It was showed by SDS PAGE that the protein molecular weight of the coexpression product was about 20 kD,20% of all bacteria protein.The monoclonal antibodies against core and preS1 antibody could react with this fused protein by Western blot technique respectively.The fused gene was verified by sequencing.Under the immune electron microscopy,this fused protein is typical particles of HBcAg but in an aggregated form.Conclusion.The results might aid for studying T cell immunotherapeutic vaccine for chronic hepatitis B.

  • 作者:董菁;成军;王勤环;施双双;王刚;斯崇文

    Objective.To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat.Methods.Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome.Results.A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified.The lengths of the gene and pseudogene are 1508 bp and 442 bp,respectively.The ALR gene of rat includes 3 exons and 2 introns.The 442 bp DNA sequence may represent a pseudogene or a ALR related peptide.Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene.ALR related peptide is 84 amino acid residues in length and relates closely to ALR protein.Conclusion.There might be a multigene family of ALR in rat.

  • 作者:陈文明;吴垠;朱嘉芷;刘敬忠;谭淑珍;夏成青

    Objective.To investigate the frequency of p16 and p15 gene methylation in multiple myeloma (MM),and its relationship with bone marrow cell apoptosis and clinical outcome.Methods.Twenty two patients with MM were studied to detect p16 and p15 gene methylation.Methylation specific polymerase chain reaction (MSP) was used to detect gene methylation,and terminal transferase mediated dUTP nick end labeling (TUNEL) was used to detect cell apoptosis.Results.p16 and /or p15 gene methylatoin was detected in 10 of 22 patients (45.4%).There were 3 patients with p16 gene methylation,9 patients with p15 gene methylation,and 2 patients with both genes methylation.The incidence of methylation of p15 gene was higher than that of p16 gene (P< 0.05).The patients with p16 and/or p15 gene methylation had a delayed cell apoptosis,poor response to chemotherapy,and a short overall survival (OS).Conclusion.The methylation of p16 and/or p15 gene plays a key role in MM apoptosis pathogenesis.The patients with both p16 and p15 gene methylation had a poor prognosis.

  • 作者:付小兵;杨银辉;孙同柱;王亚平;盛志勇

    Objective.To explore the expression characteristic of fibronectin gene in hypertrophic scars and diabetic ulcer tissues.Methods.The biopsies from normal skins,hypertrophic scars and diabetic foot ulcers were taken.The technique of quantitative polymerase chain reaction (PCR) was used to evaluate the gene expression of fibronectin in the above biopsies.Results.Fibronectin gene expression was enhanced in hypertrophic scars and decreased in diabetic foot ulcers compared with that in normal skins.Quantitative comparison showed about 2 fold increase of fibronectin mRNA level in hypertrophic scars and about 3 fold decrease of fibronectin mRNA level in diabetic ulcers as compared with that in normal skins.Conclusions.Fibronectin gene expression is influenced by the tissue environment.Different expression and synthesis of fibronectin may cause different outcomes in wound healing.

  • 作者:鲍朗;邱洪宇;晏菊芳;谢勇恩;陈玮

    Objective.To construct recombinant BCG against leptospirosis.Methods.We amplified the entire open reading frame of the OmpL1 gene from the genome of the leptospire serovar Lai strain 017.Two recombinant plasmids pBQ1 and pBQ2 were constructed by oriented ligation based on the E.coli BCG shuttle plasmids pMV261 and pMV361 respectively.The recombinant plasmids were transformed into BCG by electroporation.The rBCGs bearing pBQ1 and pBQ2 were induced by high temperature of 45℃ .Results.The expressed product,a 35kD protein was detected by SDS PAGE.The result indicates that pBQ1 and pBQ2 can express OmpL1 in rBCG.Conclusion.The technical methods in this study may help detect the immunogenicity and immunoprotection of OmpL1 and develop more safe,highly effective rBCG bearing leptospiral antigen with long lasting protection.

  • 作者:杨佳欣;刘俊涛;边旭明

    Objective.To analyze the clinical characteristics of peripartum cardiomyopathy and to evaluate the different factors that influence the prognosis of the peripartum cardiomyopathy.Method.A retrospective review was undertaken on records of women who were diagnosed with peripartum cardiomyopathy at Peking Union Medical College Hospital between Jan.1983 and May 1999.Results.During the research period,only 16 pregnant women were documented as peripartum cardiomyopathy.Some of the women undertook ultrasonic cardiographic (UCG) examination that showed decreased systolic function.Seven women were complicated with pregnancy induced hypertension.Three died of disseminated intravascular coagulation,embolism and cardiogenic shock respectively.Conclusion.Early diagnosis of the peripartum cardiomyopathy is extremely important.The UCG can provide helpful information on disease progression or regression.

  • 作者:傅晨薇;郎景和

    Objective.To investigate the serum sE cadherin level in patients with endometriosis and the alterations of that level in healthy control during the menstrual cycle.Methods.Thirty two patients with endometriosis and 30 healthy women were tested for serum sE cadherin levels by enzyme linked immunosorbent assay.Results.The serum sE cadherin levels in healthy control did not vary throughout the menstrual cycle,which were lower than those in patients with endometriosis.Conclusions.E cadherin might be involved in endometrial shedding during menstruation in endometriosis patients.The serum sE cadherin assay might be helpful as a serum marker for the diagnosis and management of endometriosis.

    关键词: endometriosis Cadherin
  • 作者:丁育基;李慎茂;段安安;于小千;华杨;柳江;王建生;曹家康;赵瑞林;徐庚;谷春;王仲朴

    Objective.To summarize the experience of minimally invasive treatment in 520 patients with intracranial aneurysms on a retrospective study.Methods.The measures used in the treatment of 520 patients were reviewed in terms of timing of surgery,induced hypotensive anesthesia,brain protection combined with temporal occlusion of the feeding artery,external drainage of CSF,dynamic monitoring of intracranial pressure,blood flow velocity,serum osmolality and CT scanning,anti vasospasm therapy as well as selected interventional endovascular embolization of aneurysms.Results.Of the 520 patients,485 were treated with either direct clipping or endovascular embolization and 35 patients were treated non surgically.In 449 patients undergoing direct clipping and 36 undergoing endovascular embolization,intraoperative rupture of aneurysm occurred in 27 (6.0%) and 0%,respectively.Death occurred in 13 (2.6%),hemiplegia in 8 (1.6%),and vegetative state in 2 (0.4%).The operative mortality of direct clipping was 3.8% in 210 patients before 1990 and 1.8% in 275 patients after 1990 (36 patients undergoing endovascular embolization,the operative mortality was 0%).Conclusion.The outcome of patients with intracranial aneurysms can be markedly improved and the operative mortality can be lowered by minimally invasive treatment.

  • 作者:刘爱军;陈乐真;颜婉嫦;邱玮璇;赵昀;张雅贤

    Objective.To determine the apoptotic and proliferative activities in various ovarian epithelial tumors.Methods.Formalin fixed,paraffin embedded tissues of 86 ovarian epithelial tumors,including 52 adenocarcinomas,23 borderline tumors and 11 cystadenomas,were retrieved.Apoptotic (AI) and proliferative (PI) index were estimated using the monoclonal antibodies: M30,Ki 67 and Ki S1 in these tumors.Quantitative assessment of AI and PI was estimated by calculating the percentage of positive cells among no less than 1000 tumor cells.Results.Statistically significant difference in AI was found between benign and borderline tumors or carcinomas (P=0.028,0.001,respectively).Significant differences in PI,as assessed by both Ki 67 and topo IIα,were demonstrated between carcinomas and benign or borderline tumors (both P< 0.001).Benign tumors had both low PI and AI; borderline tumors had lower PI but higher AI,while adenocarcinomas had both high proliferative and high apoptotic rates.Among borderline tumors,serous tumors had significantly lower AI and higher PI than mucinous ones.Conclusions.The results suggest that apoptotic and proliferative activities play important roles in the pathogenesis and development of ovarian borderline and malignant tumors.The high apoptotic rate in borderline tumor may explain its relatively indolent behavior while the high proliferative rate in carcinomas tends to explain its aggressive behavior.

  • 作者:崔璨;李强;张一娜;张巾超

    Objective.To identify the A3243G mutation of mitochondrial(mt) DNA in patients with latent autoimmune diabetes mellitus in adults (LADA) of Han nationality in the northeast area of China. Methods.Seventy nine diabetics of Han nationality,whose families have resided in the northeast area of China for more than 3 generations,were divided into 3 groups: Group 1 (22 cases of type 2 diabetes with maternal inheritance history),Group 2 (34 cases of LADA),Group 3 (23 cases of type 1 diabetes in adolescents).The A3243G of mt DNA was detected in these 79 subjects with the method of PCR RFLP. Results.None of the 79 diabetics studied was positively identified for the A3243G mutation of mt DNA. Conclusion.The A3243G mutation of mt DNA might not be related to the onset of LADA in diabetic population of Han nationality in northeast area of China and there might not be close relationship between A3243G mutation of mt DNA and autoimmunity.

  • 作者:叶平;胡晓晖;赵亚力

    Objective.To investigate the effect of peroxisome proliferator activated receptors (PPARs) activators on plasminogen activator inhibitor type 1 (PAI 1) expression in human umbilical vein endothelial cells and the possible mechanism.Methods.Human umbilical vein endothelial cells (HUVECs) were obtained from normal fetus,and cultured conventionally.Then the HUVECs were exposed to test agents (linolenic acid,linoleic acid,oleic acid,stearic acid and prostaglandin J2 respectively) in varying concentrations with fresh media.RT- PCR and ELISA were applied to determine the expression of PPARs and PAI 1 in HUVECs.Results.PPAR α,PPAR δ and PPAR γ mRNA were detected by using RT PCR in HUVECs.Treatment of HUVECs with PPARα and PPAR γ activators- - linolenic acid,linoleic acid,oleic acid and prostaglandin J2 respectively,but not with stearic acid could augment PAI I mRNA expression and protein secretion in a concentration dependent manner.However,the mRNA expressions of 3 subclasses of PPAR with their activators in HUVECs were not changed compared with controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI 1 expression in ECs,but the underlying mechanism remains unclear.Although PPARs expression was not enhanced after stimulated by their activators in ECs,the role of functionally active PPARs in regulating PAI 1 expression in ECs needs to be further investigated by using transient gene transfection assay.

  • 作者:黄宇光;杨克勤;任洪智;罗爱伦

    Objective.To assess patients' knowledge,attitudes,and concerns regarding anesthetic management.Method.A survey of 55 items was developed and administered preoperatively to 500 patients including 190 men and 310 women in our hospital.Patients were interviewed on their knowledge of the role of anesthesiologists,their preferences regarding anesthetic management,and also their concern about potential anesthetic complications.Results.Patients' perceptions of anesthesiologists' training and role have reached a certain level.Most significant preoperative concerns regarding the anesthesiologists focused on experience,qualifications,and presence or absence during the anesthesia.Patients' concerns also included the possibility of not being waken up following anesthesia,experiencing postoperative pain,and becoming paralyzed.The variations of concerns depended partially on patients' sex,type of anesthesia,and proposed surgical procedure,partially on their education and living environments.Conclusion.It is suggested that anesthesiologists address significant patient concerns during the preoperative visit to enhance their effectiveness in patient care.Efforts to educate the public on the anesthesiologists' role in preoperative care would improve patients' confidence.

  • 作者:周维新;Rainer Hoffmann;Andreas Franke;杨浣宜;Harald Kuhl;Peter Hanrath

    Objective.It was suggested that coronary stent design and coating may affect stent performance and hence induce varying degrees of thrombogenesis and neointimal hyperplasia.The purpose of this study is to compare the 6 month follow up results between old and new Multilink stents with the method of intravascular ultrasound (IVUS) imaging.Methods.We have performed old (n=40) and new (n=35) Multilink stent implantations on 75 patients with coronary artery disease.Coronary angiography was performed before,immediately after,and 6 months after the in stent procedure respectively.Six month follow up IVUS imaging was performed and analyzed off line.Results.Minimal lumen cross sectional area (CSA) of new Multilink stents was significantly larger than that of old Multilink stents (P=0.0053).Mean stent lumen area of new Multilink stents was significantly larger than that of old Multilink stents (P=0.040).Similarly,minimal lumen diameter (MLD) of new Multilink stents was larger than that of old Multilink stents (P= 0.011).Old Multilink stents had a higher percentage of plaque area than new Multilink stents.Conclusion.The new Multilink stent is obviously superior to old Multilink stents,in particular,in the stent MLD and lumen CSA- - major determinants of the restenosis.

  • 作者:蔡昌豪;王孟薇

    To get a better understanding of the location,pathophysiology,etiology and prognosis of multiple malignant tumors (MPMT),we evaluated the medical records of 22 patients with MPMT.Our results suggested that radiotherapy and chemotherapy might play an important role in the pathogenesis of MPMT and follow up is important in detecting a secondary primary malignant tumor (PMT) at an early stage.Surgical removal of tumors is the first choice therapy for MPMT.

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