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  • 3D生物打印兔肺成纤维细胞水凝胶结构体片段

    作者:罗涛;杨亚冬;杨耿;唐靓;李跃中;张文元

    目的 通过三维(3D)生物打印兔肺成纤维细胞-海藻酸钠-明胶共混物,探讨3D生物打印技术构建肺成纤维细胞水凝胶结构体片段的可行性.方法 用轻度Ⅳ型胶原酶消化结合组织块培养法分离纯化新生兔肺成纤维细胞,并对分离纯化的肺成纤维细胞进行原代和传代培养;制备海藻酸钠-明胶水溶胶,与兔肺成纤维细胞共混;用圆柱状微丝逐层交错堆积技术,进行兔肺成纤维细胞-海藻酸钠-明胶共混物的3D生物打印,获得三维结构体.用钙黄绿素-AM和碘化丙啶溶液对该结构体进行活/死细胞双荧光染色,评价打印后及培养后兔肺成纤维细胞的活性,计算细胞存活率.结果 通过按照设计的参数,打印出含有兔肺成纤维细胞的网格状水凝胶结构体.该结构体的大小约为9 mm×9 mm×1.2 mm,微丝间的孔隙相互通连,孔隙大小约600μm×600μm.打印后的兔肺成纤维细胞在三维结构体中仍可增殖,活/死细胞荧光染色结果表明结构体中兔肺成纤维细胞的存活率约为84%±3%,兔肺成纤维细胞在结构体中分布均匀.结论 本实验实现了兔肺成纤维细胞共混物结构体的3D生物打印,为构建类肺组织工程结构体片段提供新思路.

  • 3D生物打印技术在构建兔原代肝细胞水凝胶结构体片段上的研究

    作者:罗涛;唐靓;杨亚冬;张文元

    目的 通过三维(3D)生物打印兔原代肝细胞-海藻酸钠-明胶共混物,探讨3D生物打印技术构建类肝水凝胶结构体片段的可行性.方法 采用Ⅳ型胶原酶消化法提取分离新生兔原代肝细胞;制备海藻酸钠-明胶水溶胶,与兔原代肝细胞共混;采用圆柱状微丝逐层交错堆积技术,进行兔肝细胞-海藻酸钠-明胶共混物的3D生物打印,获得三维结构体.用钙黄绿素-AM和碘化丙啶溶液对该结构体进行活/死细胞双荧光染色,评价打印后及培养后兔肝细胞的活性,计算细胞存活率.结果 通过按照设计的参数,打印出含有兔原代肝细胞的网格状水凝胶结构体.该结构体的规格为10mm×10mm×1.2 mm,微丝间的孔隙相互通连,孔隙大小约450 μm×450 μm.打印后的兔肝细胞在三维结构体中仍可增殖,活/死细胞荧光染色结果表明结构体中兔肝细胞的存活率为82%±3%,兔原代肝细胞在结构体中分布均匀.结论 本研究实现了兔肝细胞共混物结构体的3D生物打印,为构建类肝组织工程结构体片段提供新思路.

  • 三维打印技术在皮肤组织修复上的应用

    作者:林越威

    三维打印是一种低成本、高效益的生产方法,有助于改善目前皮肤修复材料产业成本高昂、利润放缓的趋势,并开发出性能更优秀的产品.常用于皮肤修复材料制备中的三维打印技术有熔融沉积成型技术和三维生物打印技术:其中熔融沉积成型技术具有设备简单轻巧的优点,但材料选择不足;三维生物打印技术可供选择的材料较多,但设备笨重且昂贵.近年对两种技术的研究均集中于材料的开发和应用上.本文详细介绍了熔融沉积成型技术和三维生物打印技术的原理、在伤口敷料和组织工程皮肤生产上的研究进展,以及三维打印在皮肤组织修复上的未来发展,包括容貌修复和仿生组织工程皮肤的研究,并对三维打印技术应用在皮肤组织修复领域的发展进行了展望.

  • 三维生物打印在皮肤组织工程中的应用研究进展

    作者:陈浩娇;吴攀;王新刚;韩春茂

    Deep skin wounds require skin grafts for coverage.Current treatments such as tissue engineered skin or skin substitutes can not meet the needs of clinical application due to the technical problems involving preservation,transportation,and a lengthy preparation process.In comparison with traditional methods such as freeze-drying,three-dimensional bioprinting can precisely dispense living cells,nucleic acid,growth factor,and phase-changing hydrogels according to the wound form,while maintaining high cell viability.Besides,it has excellent performance in high resolution,flexibility,reproducibility,and high throughput,showing great potential in the fabrication of tissue engineered skin.This review mainly introduces the common techniques of three-dimensional bioprinting,and their application in skin tissue engineering,focusing on the latest research progress,and summarizes the current challenges and future development of three-dimensional skin printing.

  • 三维生物打印在烧伤整形领域中的应用进展

    作者:李如兵;李敏雄;郭光华;张红艳

    Three-dimensional bioprinting is one of the latest and fastest growing technologies in the medical field.It has been implemented to print part of the transplantable tissues and organs,such as skin,ear,and bone.This paper introduces the application status,challenges,and application prospect of three-dimensional bioprinting in burn and plastic surgery field.

  • 水凝胶在三维生物打印中的应用研究进展

    作者:杨景;赵洋;李海航;朱世辉

  • 胶原蛋白在三维生物打印中的应用研究进展

    作者:李海航;罗鹏飞;盛嘉隽;刘功成;朱世辉

    As a new industrial technology with characteristics of high precision and accuracy,the application of three-dimensional bioprinting technology is increasingly wide in the field of medical research.Collagen is one of the most common ingredients in tissue,and it has good biological material properties.There are many reports of using collagen as main composition of " ink" of three-dimensional bioprinting technology.However,the applied collagen is mainly from heterogeneous sources,which may cause some problems in application.Recombinant human source collagen can be obtained from microorganism fermentation by transgenic technology,but more research should be done to confirm its property.This article reviews the advances in the research of collagen and its biological application in three-dimensional bioprinting.

  • 三维生物打印微环境诱导汗腺再生的研究

    作者:姚斌;谢江帆;黄沙;付小兵

    Sweat glands are abundant in the body surface and essential for thermoregulation.Sweat glands fail to conduct self-repair in patients with large area of burn and trauma,and the body temperature of patients increases in hot climate,which may cause shock or even death.Now,co-culture system,reprogramming,and tissue engineering have made progresses in inducing sweat gland regeneration,but the inductive efficiency and duration need to be improved.Cellular microenvironment can regulate cell biological behavior,including cell migration and cell differentiation.This article reviews the studies of establishment of microenvironment in vitro by three-dimensional bioprinting technology to induce sweat gland regeneration.

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