发表一篇学和医学成像类SCI论文
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Abstract:
BACKGROUND:Hepatitis B virus (HBV) polymerase is the only virus-encoded enzyme essential for producing the HBV genome and is regarded as an attractive drug target. However, the difficulty of synthesizing and purifying recombinant HBV polymerase protein has hampered the development of new drugs targeting this enzyme, especially compounds unrelated to the nucleoside structure. We recently have developed a technique for the synthesis and purification of recombinant HBV polymerase containing the reverse transcriptase (RT) domain that carried DNA elongation activity in vitro. METHODS:We used the overproduced protein to establish an in vitro high-throughput screening system to identify compounds that inhibit the elongation activity of HBV polymerase. RESULTS:We screened 1120 compounds and identified a stilbene derivative, piceatannol, as a potential anti-HBV agent. Derivative analysis identified another stilbene derivative, PDM2, that was able to inhibit HBV replication with an IC50 of 14.4 ± 7.7 μM. An infection experiment suggested that the compounds inhibit the replication of HBV rather than the entry process, as expected. Surface plasmon resonance analysis demonstrated a specific interaction between PDM2 and the RT domain. Importantly, PDM2 showed similar inhibitory activity against the replication of both wild-type HBV and a lamivudine/entecavir-resistant HBV variant. Furthermore, PDM2 showed an additive effect in combination with clinically used nucleos(t)ide analogs. CONCLUSIONS:We report the development of a screening system that is useful for identifying non-nucleos(t)ide RT inhibitors.
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最新影响因子:6.772 | 期刊ISSN:0944-1174 | CiteScore:4.51 |
出版周期:Monthly | 是否OA:YES | 出版年份:0 |
期刊官方网址:http://www.springer.com/medicine/internal/journal/535
自引率:5.10% | 研究方向:医学-胃肠肝病学 |
出版地区:JAPAN |
SCI期刊coverage:Science Citation Index Expanded(科学引文索引扩展)
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The Journal of Gastroenterology which is the official publication of the Japanese Society of Gastroenterology publishes original papers case reports reports of multi-center trials review articles short and rapid communications and other articles on all aspects of the field of gastroenterology. Significant contributions relating to basic research theory and practice are welcomed. This publication is designed to disseminate knowledge in this field to a worldwide audience and accordingly its editorial board has an international membership.
《胃肠病学杂志》是日本胃肠病学学会的官方刊物,发表多中心临床试验的原创性论文、病例报告、综述报告、简短快速的交流文章等,涉及胃肠病学领域的各个方面。欢迎在基础研究、理论和实践方面作出重大贡献。本刊物旨在向全世界读者传播这方面的知识,因此其编辑委员会具有国际成员资格。
大类(学科) | 小类(学科) | 学科排名 |
医学 |
GASTROENTEROLOGY & HEPATOLOGY (胃肠肝病学) 2区 |
12/80 |
年度总发文量 | 年度论文发表量 | 年度综述发表量 |
120 | 99 | 21 |
引文计数(2018)
文献(2015-2017)
1903次引用
422篇文献
序号 | 类别 | 排名 | 百分位 |
1 |
大类(学科):Medicine
小类(学科):Gastroenterology
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#7/128
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研究方向:消化病 胃肠道
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研究方向:肠道
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研究方向:消化病 胃肠道
影响因子:2.566
ISSN:0739-7240
研究方向:农林科学-奶制品与动物科学
影响因子:6.497
ISSN:1172-7047
研究方向:医学-精神病学
影响因子:1.297
ISSN:0028-2804
研究方向:医学-精神病学
影响因子:4.604
ISSN:1092-8529
研究方向:医学-精神病学
影响因子:5.345
ISSN:1398-5647
研究方向:医学-精神病学
影响因子:3.492
ISSN:1540-2002
研究方向:CLINICAL NEUROLOGY-PSYCHIATRY
影响因子:4.562
ISSN:0269-8811
研究方向:医学-精神病学
影响因子:6.533
ISSN:0165-0327
研究方向:医学-精神病学
影响因子:5.415
ISSN:0924-977X
研究方向:医学-精神病学
发表一篇学和医学成像类SCI论文
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