N-Linked Glycans and K147 Residue on Hemagglutinin Synergize To Elicit Broadly Reactive H1N1 Influenza Virus Antibodies.

Abstract:
:Vaccination is the most effective way to prevent influenza virus infections. However, the diversity of antigenically distinct isolates is a challenge for vaccine development. In order to overcome the antigenic variability and improve the protective efficacy of influenza vaccines, our research group has pioneered the development of computationally optimized broadly reactive antigens (COBRA) for hemagglutinin (HA). Two candidate COBRA HA vaccines, P1 and X6, elicited antibodies with differential patterns of hemagglutination inhibition (HAI) activity against a panel of H1N1 influenza viruses. In order to better understand how these HA antigens elicit broadly reactive immune responses, epitopes in the Cb, Sa, or Sb antigenic sites of seasonal-like and pandemic-like wild-type or COBRA HA antigens were exchanged with homologous regions in the COBRA HA proteins to determine which regions and residues were responsible for the elicited antibody profile. Mice were vaccinated with virus-like particles (VLPs) expressing one of the 12 modified HA antigens (designated V1 to V12), COBRA HA antigens, or wild-type HA antigens. The elicited antisera was assessed for hemagglutination inhibition activity against a panel of historical seasonal-like and pandemic-like H1N1 influenza viruses. Primarily, the pattern of glycosylation sites and residues in the Sa antigenic region, around the receptor binding site (RBS), served as signatures for the elicitation of broadly reactive antibodies by these HA immunogens. Mice were vaccinated with VLPs expressing HA antigens that lacked a glycosylation site at residue 144 and a deleted lysine at position 147 residue were more effective at protecting against morbidity and mortality following infection with pandemic-like and seasonal-like H1N1 influenza viruses.IMPORTANCE There is a great need to develop broadly reactive or universal vaccines against influenza viruses. Advanced, next-generation hemagglutinin (HA) head-based vaccines that elicit protective antibodies against H1N1 influenza viruses have been developed. This study focused on understanding the specific amino acids around the receptor binding site (RBS) that were important in elicitation of these broadly reactive antibodies. Specific glycan sites and amino acids located at the tip of the HA molecule enhanced the elicitation of these broadly reactive antibodies. A better understanding of the HA structures around the RBS will lead to more effective HA immunogens.
展开更多
JOURNAL OF VIROLOGY
J VIROL
最新影响因子:6.549 | 期刊ISSN:0022-538X | CiteScore:4.24 |
出版周期:Semimonthly | 是否OA:YES | 出版年份:1967 |
期刊官方网址:http://jvi.asm.org/
自引率:12.30% | 研究方向:医学-病毒学 |
出版地区:UNITED STATES |
SCI期刊coverage:Science Citation Index Expanded(科学引文索引扩展)
官方内部论文预审系统 :
1-3天杂志社编辑直接预审
预审通过后100%录用,3个月即可录用

微信扫码咨询
JOURNAL OF VIROLOGY 期刊简介
英文简介:
The Journal of Virology is the best source of broad-based, high-quality, original research concerning viruses. The journal provides fundamental new information using cross-disciplinary approaches of biochemistry, biophysics, cell biology, genetics, immunology, molecular biology, morphology, physiology, and pathogenesis and immunity.
中文简介:(来自Google、百度翻译)
《病毒学杂志》是关于病毒的基础广泛、高质量、原始研究的最佳来源。该杂志利用生物化学、生物物理学、细胞生物学、遗传学、免疫学、分子生物学、形态学、生理学、发病机制和免疫学等跨学科方法提供了基本的新信息。
JOURNAL OF VIROLOGY 期刊中科院评价数据
最新中科院分区
大类(学科) | 小类(学科) | 学科排名 |
医学 |
VIROLOGY(病毒学) 2区 |
8/35 |
最新公布的期刊年发文量
年度总发文量 | 年度论文发表量 | 年度综述发表量 |
850 | 844 | 6 |
影响因子趋势图
JOURNAL OF VIROLOGY 期刊CiteScore评价数据
最新CiteScore值
引文计数(2018)
文献(2015-2017)
14616次引用
3447篇文献
CiteScore排名
序号 | 类别 | 排名 | 百分位 |
1 |
大类(学科):Immunology and Microbiology
小类(学科):Microbiology
|
#20/135
点击查看排名表
|
|
2 |
大类(学科):Immunology and Microbiology
小类(学科):Immunology
|
#39/193
点击查看排名表
|
|
3 |
大类(学科):Agricultural and Biological Sciences
小类(学科):Insect Science
|
#2/136
点击查看排名表
|
|
4 |
大类(学科):Immunology and Microbiology
小类(学科):Virology
|
#14/68
点击查看排名表
|
|
CiteScore趋势图
Scopus涵盖范围
JOURNAL OF VIROLOGY 期刊常见问题
热门常见问题
权益保障
-
刊物信息可查
推荐刊物均可到国家新闻出
版总署网站查询正刊 -
严格保密协协议
可签署保密协议 ,不透露任
何用户信息可跟踪进程,全程
协议 -
售后服务保障
1对1服务,7x24小时在线
-
企业信誉保障
12年经验沉淀,实体公司
运营
最新评论(不代表本站观点)
-
liting7111
研究方向:生命科学 兽医学 兽医免疫学
审稿时间: 1个月内
编辑效率很高,就算拒稿反馈也很快,如果有修改机会一般20天就给消息,我投稿已经接受,感谢编辑!2020-02-16 -
liting7111
研究方向:生命科学 微生物学 病原真菌学
审稿时间: 2个月内
本专业很不错的期刊,今年影响因子终于止住了跌势。2019-09-13 -
liting7111
研究方向:病毒学 免疫学
接受率: 一般容易(75%命中)
一审20天,给了小修,提的问题很难回答,好在文章比较新颖,修回三天就接收。2019-05-26 -
liting7111
研究方向:病毒 结构生物学
接受率: 比较困难(25%命中)
今年投稿两篇,第一篇拒稿,修改后再投就接收了。2019-02-03
相关期刊
-
Medical Law Review...
影响因子:2.032
ISSN:0967-0742
研究方向:MEDICAL ETHICS-
-
JOURNAL OF LAW MEDICINE & ETHICS...
影响因子:1.604
ISSN:1073-1105
研究方向:医学-医学:法
-
MEDICINE SCIENCE AND THE LAW...
影响因子:2.051
ISSN:0025-8024
研究方向:医学-医学:法
-
FOOD AND DRUG LAW JOURNAL...
影响因子:0.442
ISSN:1064-590X
研究方向:医学-食品科技
-
AMERICAN BUSINESS LAW JOURNAL...
影响因子:1.743
ISSN:0002-7766
研究方向:
-
European Business Organization Law Review...
影响因子:1.79
ISSN:1566-7529
研究方向:
-
Journal of World Energy Law & Business...
影响因子:0.403
ISSN:1754-9957
研究方向:
-
International Insolvency Review...
影响因子:0.646
ISSN:1180-0518
研究方向:
-
JOURNAL OF CRIMINAL LAW & CRIMINOLOGY...
影响因子:2.184
ISSN:0091-4169
研究方向:
JOURNAL OF VIROLOGY 投稿经验
(由下方点评分析获得,4人参与,27174人阅读)