发表一篇学和医学成像类SCI论文
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Abstract:
:Cultures of Sertoli cells isolated from 20-day-old mice are widely used in research as substitutes for adult Sertoli cell cultures. This practice is based on the fact that Sertoli cells cease to proliferate and become mature in vivo by 16 to 20 days after birth. However, it is important to verify whether cultured Sertoli cells derived from 20-day-old mice do not proliferate ex vivo and whether they have the same properties as cultured adult Sertoli cells. Herein we described an isolation/culture method of Sertoli cells from 10-week-old adult mice with > 90% purity. Properties of these cultured adult Sertoli cells were then compared with those of cultured Sertoli cells derived from 20-day-old mice (also > 90% purity). By cell counting, bromo-2-deoxyuridine incorporation, and metaphase plate detection, we demonstrated that only adult Sertoli cells did not proliferate throughout 12 culture days. In contrast, Sertoli cells derived from 20-day-old mice still proliferated until Day 10 in culture. The morphology and profiles of intracellular lipidomics and spent medium proteomics of the 2 cultures were also different. Cultured adult Sertoli cells were larger in size and contained higher levels of triacylglycerols, cholesteryl esters, and seminolipid, and the proteins in their spent medium were mainly engaged in cellular metabolism. In contrast, proteins involved in cell division, including anti-Mullerian hormone, cell division cycle protein 42 (CDC42), and collagen isoforms, were at higher levels in Sertoli cell cultures derived from 20-day-old mice. Therefore, cultured Sertoli cells derived from 10-week-old mice, rather than those from 20-day-old animals, should be used for studies on properties of adult Sertoli cells.
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最新影响因子:5.051 | 期刊ISSN:0013-7227 | CiteScore:3.66 |
出版周期:Monthly | 是否OA:YES | 出版年份:0 |
自引率:5.70% | 研究方向:医学-内分泌学与代谢 |
出版地区:UNITED STATES |
SCI期刊coverage:Science Citation Index Expanded(科学引文索引扩展)
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Endocrinology, the Society's oldest journal, has defined the science of endocrinology for most of the twentieth century. One of the most authoritative biomedical research journals in the world, it publishes 6,000 pages annually of the highest quality original work ranging from subcellular mechanisms to whole animal physiology. Topics include bone and mineral; growth factors; reproductive/steroids; neuroendocrinology/signal transduction; thyroid; and physiology. The low manuscript acceptance rate of 30% reflects the degree to which it is committed to the highest scientific standard. It is ranked #13 out of 89 endocrinology and metabolism journals by ISI.
内分泌学,这个协会最古老的期刊,在20世纪的大部分时间里定义了内分泌学的科学。它是世界上最权威的生物医学研究期刊之一,每年出版6000页高质量的原创作品,从亚细胞机制到整个动物生理学。主题包括骨骼和矿物质;生长因子;生殖/类固醇;神经内分泌学/信号转导;甲状腺;和生理学。较低的30%的稿件通过率反映了它致力于最高科学标准的程度。它在ISI发布的89份内分泌和代谢杂志中排名第13位。
大类(学科) | 小类(学科) | 学科排名 |
医学 |
ENDOCRINOLOGY & METABOLISM (内分泌学与代谢) 3区 |
38/142 |
年度总发文量 | 年度论文发表量 | 年度综述发表量 |
327 | 306 | 21 |
引文计数(2018)
文献(2015-2017)
5127次引用
1401篇文献
序号 | 类别 | 排名 | 百分位 |
1 |
大类(学科):Biochemistry, Genetics and Molecular Biology
小类(学科):Endocrinology
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#24/119
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研究方向:基础研究
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研究方向:基础
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研究方向:医药科学 内分泌系统 代谢和营养支持 内分泌系统炎症与感染
审稿时间: 1个月内
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影响因子:2.293
ISSN:0962-7286
研究方向:农林科学-动物学
影响因子:1.083
ISSN:0932-0814
研究方向:农林科学-动物学
影响因子:0.65
ISSN:0008-1078
研究方向:农林科学-动物学
影响因子:0.8
ISSN:0046-9939
研究方向:FISHERIES-ZOOLOGY
影响因子:0.913
ISSN:0137-1592
研究方向:FISHERIES-ZOOLOGY
影响因子:3.605
ISSN:0145-305X
研究方向:医学-动物学
影响因子:1.565
ISSN:1532-0820
研究方向:医学-动物学
影响因子:0.362
ISSN:0324-0770
研究方向:ZOOLOGY-
影响因子:0.653
ISSN:1525-2647
研究方向:医学-动物学
发表一篇学和医学成像类SCI论文
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