基础医学与临床杂志
Basic & Clinical Medicine 기초의학여림상
- 主管单位： 北京市科学技术协会
- 主办单位： 北京生理科学会
- 影响因子： 0.66
- 审稿时间： 1-3个月
- 国际刊号： 82-358
- 国内刊号： 1001-6325
- 论文标题 期刊级别 审稿状态
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羊的膜肺过程对头孢拉定药代动力学的影响
观察膜肺(ECMO)过程对羊头孢拉定药代动力学的影响.通过在ECMO前和ECMO中对8只绵羊静注头孢拉定,观察药物在动物体内的分布和代谢情况.与ECMO前自身对照比较,ECMO中药动力参数表现为清除相半衰期(T1/2b)延长(38.1±6.1min vs 69.8±17.0min,P<0.01),总表观分布容积(Vd)增大(0.30±0.12L/kg vs 0.46±0.18L/kg,P<0.01),而清除率(Cl)等其它参数无明显改变.体外循环中膜氧合器前后头孢拉定血药浓度测定结果无任何差异.(1)ECMO治疗过程并不影响药物清除率,清除相半衰期延长可能来源于体外循环所致的总表观分布容积增大.(2)膜氧合器本身对药物并无清除作用.
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人白细胞抗原DQB15'-调控区序列与I型糖尿病的关联
应用聚合酶链反应(PCR)、PCR/SSCP和克隆测序等方法比较分析汉族1-型糖尿病患者与正常对照者HLA-DQB1 5'-调控区多态性.结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同,且与白种人基因结构一致;y box核苷酸序列存在二种结构,CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型;转录起始位点至y box间-44至-61位存在多态性,-59至-61位AAG等位基因可能与1-型糖尿病易感相关联;在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变.表明HLA- DQB1基因5'-调控区主要作用元件旁侧核苷酸变异可能参与1-型糖尿病的病因学.
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兔膈肌肌浆网Ca2+-ATPase活性及钙离子的转运
本文分别用定磷法测定兔膈肌SR Ca2+-APTase活性、Fura-2荧光法测定SR Ca2+释放、摄取动力学和[3H]-Ryanodine 与RyR结合实验测定SR RyR的量,分析其功能特性.结果显示兔隔肌、心肌和骨骼肌SR Ca2+-APTase 活性分别为70.13±8.25、41.25±6.25和120.17±17.03mmol/L pi/mg 蛋白/ h1.膈肌的SR Ca2+-APTase 活性显著高于心肌(P<0.01),但明显低于骨骼肌(P<0.01);膈肌SR Ca2+释放量和摄取速度显著快于心肌(P<0.01),但明显低于骨骼肌(P<0.01);膈肌SR RyR同[3H]Ryanodine的大结合值(Bmax)是0.78±0.05pmol/mg 蛋白, 其解离常数(KD)是6.93±1.13nmol/L , 分别位于心肌和骨骼肌范围内.本文认为膈肌Ca2+ 释放单位、 SR Ca2+-APTase 和SR Ca 2+释放摄取动力学分别具有心肌和骨骼肌的一些特征,其Ca2+ 释放可能具有变构偶联和CICR偶联两种形式, 心肌型DHPR亚型,RyR3和SERCA2a的存在可能是膈肌ECC依赖于细胞外Ca2+的主要原因.
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自身抗原SSA/Ro-52kD抗原优势表位分析
探讨自身抗原SSA/Ro-52kD的抗原优势表位,为疾病机制的研究提供依据.根据计算机软件进行的蛋白质序列结构分析,采用PCR法克隆自身抗原SSA/Ro-52kD多肽片段的cDNA,定向插入表达载体PGEX-5X-1,并且导入大肠杆菌中表达重组融合蛋白,用GST亲和层析柱进行纯化,经免疫印迹法与病人阳性血清进行反应.结果表明片段Ro523具有较强的抗原性.这提示SSA/Ro-52kD 的抗原优势表位主要存在于170~270位.
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丙型肝炎病毒核心基因疫苗诱导小鼠细胞及体液免疫应答
为了探索丙型肝炎病毒核心区基因疫苗对小白鼠细胞及体液免疫功能的影响,本文将HCV核心区基因定向克隆于质粒pcDNA3巨细胞病毒启动子下游,构建了能在真核细胞内表达的重组基因疫苗pcDNA3-HCV,将此疫苗通过多点肌肉注射免疫BALB/C小鼠,结果发现接种了该疫苗的小鼠出现抗-HCV IgG阳性,其抗体水平明显高于对照组,同时Th1免疫应答相关的细胞因子IL-2及g干扰素水平也明显高于对照组,其结果表明,HCV核心基因疫苗能诱导BACB/L小鼠产生良好的细胞及体液免疫应答.
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大鼠部分后根损伤诱发机械性痛性感觉异常的交感神经依赖性
建立一个类似临床的后根损伤诱发慢性痛的动物模型,探讨交感神经活动在诱发慢性痛中的作用.在腰5部分后根结扎损伤的方法建立一个后根损伤大鼠模型,观察损伤诱发的慢性痛形成的行为和电生理学特征及交感节后神经元在慢性痛形成中的作用.结果表明:(1)腰5部分后根结扎损伤后,损伤侧后肢出现机械性痛性感觉异常,持续约30天.(2)提前12天行腰交感神经干和神经节切除术,部分后根损伤侧后肢不出现机械性痛性感觉异常.(3)腹腔注射肾上腺素能a受体阻断剂酚妥拉明(4mg/kg)使机械性痛性感觉异常显著降低,维持约30min.(4)后根的部分损伤诱发损伤神经21.7%(162/746)的A类纤维来自背根节细胞的异位放电活动;全身或局部应用去甲肾上腺素可使异常自发电活动产生改变,其中64.1%的(25/39)A类纤维兴奋,33.3%(13/39)的A类纤维抑制.提示交感神经参与后根损伤诱发的机械性痛性感觉异常.
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蟾蜍灵诱导人胃癌细胞凋亡的实验研究
用MTT 法、形态学观察法、 DNA 电泳和流式细胞仪观察蟾蜍灵(Bufalin)诱导的胃癌细胞凋亡.结果表明,0.01mmol/L 蟾蜍灵作用 MGc-803细胞48 h,即可显著抑制细胞生长,IC50值约为0.1 mmol/L.琼脂糖凝胶电泳获得梯形图谱,流式细胞仪DNA直方图上出现典型的亚二倍体峰,凋亡主要发生在G1/G0期,细胞凋亡率随药物浓度和作用时间的增加而增高.以上结果提示,bufalin对胃癌细胞具有很强的抑制作用,诱导凋亡是其主要作用机制之一.
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P-450c17mRNA在原发性醛固酮增多症肾上腺皮质腺瘤及增生组织中的表达及意义
为探讨细胞色素P-450c17-羟化酶(P-450c17)mRNA在原发性醛固酮增多症(原醛)肾上腺皮质腺瘤(APA)及增生组织中的表达及其意义,采用 Northern Blot法对7例APA组织,3例结节样增生组织,7例正常肾上腺组织,1例腹壁骨骼肌组织P-450c17 mRNA 的表达水平进行检测.结果显示P-450c17mRNA在原醛APA组织与正常肾上腺组织中的表达有显著性差异(P<0.05).P-450c17mRNA在原醛肾上腺皮质增生组织与正常肾上腺组织中的表达、在原醛APA组织与原醛肾上腺皮质增生组织中的表达相似 .提示P-450c17mRNA在APA组织中的表达降低很可能是原醛APA组织分泌过多醛固酮(ALD)的分子生物学原因.
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血管内皮生长因子基因的表达及血管生成作用
构建新的血管内皮生长因子(VEGF)高效真核表达载体pcD 2/VEGF,体外转染VSMC,发现其在VSMC中的表达效率较 pcDNA3 /VEGF明显增高,体外和体内实验证实VEGF基因具有显著促进血管生成的作用.
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人乳头瘤病毒16型转换基因的序列分析
为了解中国地区宫颈癌病人中人乳头瘤病毒16型E6E7基因结构特点, 从中国山东地区宫颈癌活检组织中提取组织DNA, 经HPV多重引物PCR法鉴定标本中感染HPV型别 ,选单纯感染HPV16型两例标本DNA为模板进行PCR扩增,获得HPV16 E6E7基因后, 重组入pALTER-1载体, 进行双向测序、分析.DNA序列分析表明: 两例标本的HPV16 E6E7序列全长均为776bp, 与已发表的德国标准株长度相等, 两例均为变异株,共检出5处核苷酸变异位点,其中4处可导致氨基酸改变.中国山东地区宫颈癌病人中HPV16 E6E7的基因结构与德国标准株的HPV16 E6E7基因之间存在差异.
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不同剂量罗沙坦对高血压大鼠冠状动脉壁肥厚的逆转作用
为研究罗沙坦对高血压冠状动脉壁肥厚的逆转作用,将16周大鼠分为自发性高血压大鼠(SHR)组、SHR口服大剂量罗沙坦组[15mg/(kg/d)]、SHR口服小剂量罗沙坦组[0.75mg/(kg.d)]和正常血压大鼠(WKY)组,饲养10周.结果显示大剂量罗沙坦治疗显著降低SHR的收缩压,降低冠状动脉壁横截面积、横截面积与内径比,提高大冠状动脉流量;小剂量罗沙坦治疗对SHR的收缩压,外径>200mm冠状动脉壁横截面积、横截面积与内径比,及大冠状动脉流量低下无影响,但能显著降低外径<200mm冠状动脉壁横截面积、横截面积与内径比.
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血管紧张素转换酶基因缺失纯合型与糖尿病肾病相关联
应用PCR扩增方法检测203例NIDDM患者和165例正常对照者血管紧张素(ACE)基因的16内含子中287bp片段缺失/插入多态性.结果显示,糖尿病肾病患者组ACE基因缺失纯合型(DD)频率明显高于非肾病组和正常对照组,P<0.001;DD缺失纯合型患者肾病进展速度明显快于缺失/插入(DI)杂合型及插入/插入(II)纯合型糖尿病患者,P<0.01.表明ACE 基因缺失纯合型是糖尿病肾病的独立危险因素之一,并可作为NIDDM肾病发生发展的有效预测指标.
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紫外线照射充氧自血回输对家兔脊髓损伤后血液循环的影响
家兔用改良Allen氏系统造成不完全性脊髓损伤,随机分为对照组、损伤组和治疗组.分别测定血液和脊髓组织TXB2、6-keto-PGF1a和ET-1含量;以及脊髓组织灰质、白质和总血流量,研究紫外线照射和充氧自血回输疗法(UBIO)治疗脊髓损伤的作用机制.实验发现:损伤组血液和脊髓组织TXB2含量升高、6-keto-PGF1a含量下降、ET-1含量升高.脊髓组织灰质和白质血流量以及脊髓组织总血流量均下降.治疗组用UBIO治疗后,血液和脊髓组织TXB2含量下降、6-keto-PGF1a含量升高、ET-1含量下降.脊髓组织灰质和白质血流量以及脊髓组织总血流量均上升.实验说明 :UBIO可以改善全身和脊髓组织的血液循环.
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粉防己碱对心肌顿抑损伤的保护作用
用兔冠脉前降支结扎缺血再灌模型,观察粉防己碱(Tet)对心肌顿抑损伤的保护作用并分析其作用机理.结果示:①顿抑心肌收缩舒张功能明显降低,心电图ST段抬高,血清LDH水平升高,左室湿重增大.Tet可减轻缺血及再灌后心肌功能的损害,降低血清LDH水平与心肌湿重;②顿抑组血清NO水平略升高,Tet组血清NO无明显变化;③顿抑组心肌Na+-k+-ATP酶及Ca2+-ATP酶活力均明显下降,Tet组ATP酶活力基本维持正常.本研究提示Tet保护顿抑心肌作用与维护心肌ATP酶的正常活性及血清NO水平的稳定有关.
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一氧化氮在脂多糖抑制血管平滑肌细胞增殖中的作用
本研究采用MTT法,3H-TdR 掺入实验、分光光度计测量和免疫组织化学的方法,观察了脂多糖对离体培养SD大鼠血管平滑肌细胞殖增、DNA合成、一氧化氮产量和一氧化氮合酶表达的影响.结果发现,脂多糖可明显抑制血管平滑肌细胞的增殖和DNA合成,推测该效应可能是通过iNOS-NO-cGMP通路实现的.
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氧化低密度脂蛋白新基因OLRG-1的克隆及组织分布
用改进的差异显示反转录-PCR技术,从血管内皮细胞中获得了一个氧化低密度脂蛋白诱导差异表达的cDNA片段.以此片段为探针,从人主动脉cDNA文库中筛选了一个新的全长cDNA.其核苷酸总长4137bp,编码642个氨基酸的蛋白质.Northern印迹分析证实氧化低密度脂蛋白诱导内皮细胞后,该基因表达减低了3倍,基因定名为OLRG-1.OLRG-1为一多组织表达的基因,与人NS1-BP基因高度同源.氧化低密度脂蛋白可能通过降低OLRG-1的表达,影响mRNA前体的剪接成熟,调控基因的表达.
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消化道癌患者胃肠道粘膜中MUC2和MUC3基因的表达
应用免疫组织化学和原位杂交方法检测人消化道癌患者胃肠道粘膜中的MUC2和MUC3基因蛋白产物和mRMA的表达,以提示MUC2和MUC3基因在人消化道癌患者胃肠道粘膜中的表达规律.结果发现,胃粘膜中无MUC2和MUC3基因产物的表达,MUC2核粘蛋白及mRNA主要存在十二指肠和结肠杯状细胞周及核上,柱状细胞中无表达.MUC3核粘蛋白及其mRNA主要存在十二指肠和结肠的杯状细胞和柱状细胞胞浆内,而杯状细胞的粘液滴内无MUC2、MUC3基因产物的阳性反应信号.提示,MUC2和MUC3基因主要在肠道粘膜中表达,而胃粘膜中不表达,是合成肠道粘液的主要粘蛋白基因.
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胫骨骨超声速率对绝经后妇女肢体骨折危险性的预测
定量骨超声是近几年新发展起来的新技术,它测定的骨超声速率(speed of sound,SOS)不仅与单光子和双能X线测定的骨密度有很好的相关性,而且还能反映与骨折有关的因素如:骨强度、骨弹性、骨脆性及骨的微结构等.因此胫骨SOS的测定对骨质疏松的诊治及对骨折危险性的预测有着重要的意义.
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维生素E对培养的肝细胞和储脂细胞细胞外基质的影响
1989年,Chojkier和Igarashi分别报告脂质过氧化可发生在未受刺激、静止的培养成纤维细胞和活体正常组织.本实验旨在了解体外培养大鼠肝细胞和储脂细胞(Ito细胞)是否存在过氧化脂质和前胶原基因表达的产物,正常培养大鼠Ito细胞有无其它细胞外基质的产生,维生素E(VE)能否抑制基础脂质过氧化和细胞外基质(ECM)产生.
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强啡肽A1-13对大鼠尾壳核注射胶原酶后脑组织水、Na+及K+含量的影响
对脑出血后脑水肿发病机理及预防的研究,一直是人们十分重视的课题.强啡肽A(DynA)属于前强啡肽原系统,在中枢神经系统分布广泛,具有多种生理作用.本室以前的研究表明,胶原酶诱导脑出血后部分脑区及垂体发生规律性改变,预先给予DynA可以保护脑出血后脑水肿,但在脑出血形成以后给予DynA,对脑出血后脑水肿有无作用尚未见报道.本研究旨在探讨DynA1-13对脑出血后脑水肿有无治疗作用.
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脂多糖对大鼠肺微血管内皮细胞形态及酶学的影响
肺微血管内皮主要由肺微血管内皮细胞和基膜组成,与ARDS、脓毒症、免疫反应、肿瘤转移和多器官功能障碍的发生密切相关.本实验主要观察脂多糖(LPS)对大鼠肺微血管内皮细胞(RPMVEC)形态及血管紧张素转换酶(ACE)、乳酸脱氢酶(LDH)活性的影响.
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蛋白酪氨酸激酶可能参与胃粘膜损伤后的胃酸分泌变化
胃酸分泌抑制是胃粘膜损伤后的一个重要现象,可能是促进胃粘膜对损伤后快速修复的一个重要因素,研究这一现象的机理有助于加深对胃粘膜损伤后修复病理生理的认识,对提高胃粘膜屏障具有重要的实践意义.
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压力超负荷大鼠左心室一氧化氮和环磷酸鸟苷含量的变化
心肌肥厚是心血管疾病发病率和死亡率的独立危险因子,压力超负荷是触发心肌肥厚反应重要的因素.一氧化氮(NO)作为一种新型的信使分子,有着广泛的心血管效应.在自发性高血压大鼠,内皮依赖性血管舒张有缺陷,L-精氨酸--NO通路异常.环磷酸鸟苷(cGMP)是血管活性因子NO和钠尿肽类等胞内信号的主要介导者.本实验用大鼠腹主动脉缩窄压力超负荷模型,观察在心肌肥厚的发生和发展过程中左心室NO和cGMP含量的变化.
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用含底物SDS-PAGE检测明胶酶活性
将明胶加入SDS-聚丙烯酰胺凝胶中,电泳后样品中所含明胶酶按分子量大小不同停留于凝胶中的特定位置.用TritonX-100除去SDS使酶复性后,明胶酶便可分解凝胶中的底物.底物被分解处考马斯亮蓝染色时不着色,因此在蓝色背景下可见酶所在位置呈白色条带,根据白色条带的深浅和宽窄可判断酶活性的高低.用此方法检查了大鼠不同组织中的明胶酶活性及传代培养对血管平滑肌细胞分泌明胶酶的影响.结果显示,大鼠不同组织及不同龄大鼠同种组织明胶酶活性存在差异.另外,传代培养的平滑肌细胞与组织间分泌明胶酶的种类亦不同.
关键词： SDS-聚丙烯酰胺凝胶电泳 明胶酶 -
采用流式细胞术检测血小板微粒及其临床应用的初探
本研究应用流式细胞仪(FCM),以3mm和0.8mm的标准微球作内参对照,建立了血小板微粒(PMPs)的定量检测方法,并对其临床应用进行了初步研究.结果表明 :30例健康人静止血小板释放的PMPs为1.2×105±5.7×104 /mL,活化血小板释放的PMPs为1.6×106±9.1×105/mL;18例冠心病(CAD)、12例急性脑梗塞(ACI)和23例慢性肾功衰竭(CRF)患者血浆中PMPs较对照组明显增高(P<0.001),分别为6.1×105±2.5×105 /mL、6.8×105±3.4×105/mL和5.9×105±3.1×105/mL,而25例急性白血病(AL)化疗后伴严重血小板减少者的PMPs 增高不明显,为1.3×105±6.1×104/mL.提示 :PMPs是监测血小板活化、预测和诊断血栓性疾病的一个重要指标,采用FCM内参定位法可以准确而快速地定量分析PMPs,有利于实验结果的室间比较和检测方法的标准化.
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人参皂甙Rg1和Rb1药理作用的比较
Ginseng has long been used as a tonic and agent for prolonging life span in chinese traditional medicine． Using morden technology，ginsenoside Rgl and Rbl were proved to be main active principles of ginseng．Both conpounds showed the same effect in improving learning and memory， increasing Bmax of M-cholinergic receptors and accelerating cerebral protein and acetylcholine biosynthesis．However，Rgl but not Rbl had immunoregulatory action in aged rats and anti-osteoporosis effect in ovariectomized rats as well as enhanced basic synaptic transmission and magnitude of LTP induced by HFS． On the other hand，Rbl had anti-stress effects in antagonizing acute,chronic and repeated stress induced reduction of sexual behaviour and decrease of plasma andogen or estrogen．Rgl showed no such effect even aggravate stress induced damage．Rhl possessed anti-oxidant activity and prolong survival time of mice in cold(－10℃) condition．There was no any anti-cold effect with Rgl .These diference of biological activities between Rgl and Rbl may be arributed to their structures containing different number of glucoses．
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六味地黄汤现代药理学及化学的初步研究
Liuwei Dihuang decoction（LW），a classical traditional Chinese medicinal prescription with the effect of “nourishing Kidney-Yin”，consists of six herbs including Rehmannia glutinosa (Shudi),Cornus officinalis(Shauzhuyu)，Dioscorea opposita(Shanyao)，Alisma orientale(Zexie)，Porie cocos（Fuling）and Paeonia suffruticosa（Mudanpi）．It has long been used clinically in the treatments of many kinds of diseases with the sign of “Kidney-Yin deficiency”，such as cancer，diabetes，autoimmue diseases，menopausal syndrome in traditional Chinese medical treatments．The effectiveness of it has been well documented during the long-term clinical practices．In our study，the effects and the possible active mechanisms of LW on learning and memory ，neuroendocrine and immune functions were studies from the angle of neuroendocrine immunomodulation（NIM）network ．In chemical fractionation and purification of the immunoactive fractions or components contained in LW，the method of a close collaboration between pharmacological and chemical studies was employed．The chemical fractionation was begun from the decoction and guided by pharmacological activity evaluation．The results showed that oral administration of LW significantly improved learning and memory abilities in several kinds of model animals such as senescence accelerated mice (SAM)，chronic hanging stress-loaded mice and corticosterone-treated mice and the mechanism studies suggested that the effects were achieved through several ways．LW significantly decreased the plasma level of corticosterone in SAM-prone/8 (SAMP8) ，a substrain of SAM．SAMP8 showed a marked elevation of plasma level of corticosterone with advancing age in comparison with SAMR1 ，a substrain of senescence resistant of SAM．LW also significantly improved the secretion of testosterone by primary cultured testis cells from SAMP8．The immunopharmacological studies showed that oral administration of LW not only improved the immune functions in several immunodeficient model animals such as cyclophospharmide-treated mice and SAMP8，but also modulated or corrected the imbalance of immune functions manifested in autoimmune model animals includig adjuvant arthritis rats and campylobacter jejuni primed mice．Under the guidance of activity evaluation，several immunoactive fractions and pure compounds were obtained from the decoction．The effects of some fractions and compounds were studied and the results showed that they possessed potential immunopharmacological effects．The preliminary study suggested that LW possessed wide pharmacological effects and the basic effect of LW，we postulate，is modulation the balance of NIM network disturbed in many pathological conditions．It is also suggested that our method employed in the study of LW is an effective and practicable way in modern studies of tradltional Chinese medicinal prescriptions．
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丹参水溶性有效成分--丹酚酸研究进展
Danshen-Radix Salviae Miltiorrhizae，is one of the common traditional Chinese medicines，which has been used clinically in the treatment of cardiovascular diseases more than 20 centuries.The active ingredients of Danshen has been researched 5 decades by the modern methods.Many researchers investigated the pharmacological effects of water soluble ingredients of Danshen-salvianolic acids in vivo and in vitro.The results demonstrated that salvianolic acids have different pharmacological effects such as potent antioxidative effects，scavenging free radicals，protect neural cells against injuries caused by anoxia， etc.In present paper，the pharmacological effects of salvianolic acids and the mechanisms of their actions are reviewed based on the research results obtained in our laboratory and other authors.
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世纪之交:现代中药药理学的回顾与展望
In the 20th Century，modern pharmacology of traditional Chinese drugs（herbal pharmacology）has been developed by Chinese pharmacologists，clinicians and related scientists using modern technology. 200～300 single active ingredients have been isolated and a number of them being used in clinics． Modern pharmacological actions of nearly 400 recipes have been investigated and a number of jounals carrying papers on herbal pharmacology have been published．The development of herbal pharmacology could be roughly divided into two periods in the 20th Century．In the first half of the Century，actions of a number of herbs have been investigated and single compounds have been isolated.The dramatic event was the isolation and action study of ephedrine done by KK Chen．In the second half of the Century，the study of herbal drugs has been carried out not only using modern technology but also under the guidance of the theory of TCM （traditional Chinese medicine）．The emphasis has been put on the sutdy of a group（or category）of herbs and recipes. Based on these studies，theories of mechanism of action of herbs is emerging．It is hoped in the next Century that the new and systematic modern herbal pharmacology will grow mature. And it is anticipated that there will be a great leap forward in the future decades．
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灵芝的抗肿瘤作用机制
Ganoderma lucidum(Leyss．Ex fr．)Karst．(Lingzhi) is a medicinal fungus with a long history in China as a valuable tonic remedy．Modern Pharmacological and clinical investigation demonstrated that Lingzhi had anti-tumor activities．Recently the antitumor effects of extract of Ganoderma lucidum(GLE) and Ganoderma polysaccharides B(GL-B) and its mechanism were investigated．The results demonstrated that GLE and GL-B significantly inhibited growth of implanted sarcoma 180 in vivo．GL-B also promoted anti-tumor activity induced by cyclophosphamide in mice in vivo．GLE and GL-B directly adding to tumor cells-cultured medium neither suppressed sarcoma 180 and HL-60 proliferation nor induced apoptosis of both tumor cells in vitro．However the serum from GLE and GL-B treated mice can suppress S-180 and HL-60 cells proliferation and induced its apoptosis in vitro．Furthermore splenocytes conditioned medium with GL-B (GL-B-S-CM) and peritoneal macrophages conditional medium with GL-B (GL-B-PM-CM) also significantly inhibited HL-60 proliferation and induced its apoptosis in vitro．Further study indicated that GLE and GL-B could promote TNFa production from murine peritoneal macrophages and IFNg production from murine spleen cells in vitro．Finally GLE and GL-B could promote TNFa mRNA expression in murine peritoneal macrophages and IFNg mRNA expression in murine spleen cells．The results suggest that the anti-tumor effect of Ganoderma lucidum relates to activating macrophage and spleen cell，then promoting TNFa mRNA expression and IFNg mRNA expression，finally resulting TNFa and IFNg release．
| 年 | 期数 |
| 2019 | 01 02 03 |
| 2018 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2017 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2016 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2015 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2014 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2013 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2012 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2011 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2010 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2009 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2008 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2007 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2006 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2005 | 01 02 03 04 05 06 07 08 09 10 11 12 |
| 2004 | 01 02 03 04 05 06 |
| 2003 | 01 02 03 04 05 06 z1 |
| 2002 | 01 02 03 04 05 06 |
| 2001 | 01 02 03 04 05 06 z1 |
| 2000 | 01 02 03 04 05 06 |
| 1999 | 03 04 05 06 Z1 |
